HIGH-THROUGHPUT SOLUTION SCATTERING DATA COLLECTION SYSTEM

高通量解决方案散射数据采集系统

• 批准号: 7954287
• 负责人: HIROTSUGU TSURUTA
• 金额: $ 1.86万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954287
• 关键词: Adopted; Air; Aliquot; Aluminum; Blood capillaries; Cells; Computer Retrieval of Information on Scientific Projects Database; Data Collection; Development; Ethanol; Flushing; Funding; Grant; Institution; Liquid substance; Manuals; Methods; Needles; Quartz; Radiation; Recovery; Research; Research Personnel; Resources; Risk; Sampling; Scheme; Series; Solutions; Source; Staging; Syringes; System; Teflon; Time; Translations; Tube; United States National Institutes of Health; Upper arm; Water; capillary; data acquisition; instrumentation; purge; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The development of instrumentation for an automated high throughput data collection system for solution x-ray scattering continues, combining a quartz capillary cell and a motorized sample changer, which combines a refrigerated aluminum block accepting any of the standard micro-plates or several PCR tube strips (up to total 8x12 tubes), with three motorized miniature linear translation stages whose vertical arm holds a syringe needle for sample aliquot selection and delivery. A Hamilton 560 fluid dispenser replaced Hamilton 540B to accommodate a larger number of fluid inlets and outlets required to accomplish sample cell cleaning, a newly automated feature. All above instrumentation are under Blu-ICE control. This year we adopted a completely new sample delivery scheme in which a syringe needle delivers a sample aliquot directly into a capillary cell instead of sending it via Teflon tubing. The new method substantially lowers the risk of generating air bubbles in the sample aliquot. A typical data collection sequence involves 1) the syringe needle picking up a sample aliquot on PCR tube strips, 2) the needle transferring the aliquot to the capillary cell, 3) repetitive translation of sample aliquot within the quartz capillary at around 1 microliter per second to minimize radiation damage, immediately followed by the initiation of a series of scattering data acquisition, 4) optional sample recovery to a PCR tube, 5) cleaning of the capillary and syringe needle by flushing them with 50% bleach and 50% ethanol solutions, then finally with distilled water; 6) drying of the capillary and syringe needle by compressed air purging. The automated sequence eliminates manual sample change, and leads to more efficient use of beam time.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 The development of instrumentation for an automated high throughput data collection system for solution x-ray scattering continues, combining a quartz capillary cell and a motorized sample changer, which combines a refrigerated aluminum block accepting any of the standard micro-plates or several PCR tube strips (up to total 8x12 tubes), with three motorized miniature linear translation stages whose vertical arm holds a syringe needle for sample aliquot选择和交付。汉密尔顿560流体分配器取代了汉密尔顿540B，以适应完成样品细胞清洁所需的大量流体入口和出口，这是新自动化的功能。上面的所有仪器均受蓝冰控制。今年，我们采用了一种全新的样品输送方案，其中注射器针直接将样品等分试样传递到毛细管细胞中，而不是通过Teflon管发送。新方法大大降低了在样品等分试样产生气泡的风险。典型的数据收集序列涉及1）注射器针上的PCR管条上的样品等分试样，2）将等分试样转移到毛细血管单元的针头，3）石英毛细管中样品等分试样重复转换为每秒1个微量尺寸的QUARTARE毛细管，每秒左右，以最大程度地减少辐射损失，紧随其后的启动position to serveriation to serveriation to serperiation to serpertiation positiation servistion persiation positiation，4）散布的启动，4）用50％的漂白剂和50％的乙醇溶液清洁毛细管和注射器针，最后用蒸馏水。 6）通过压缩空气清除毛细管和注射器针。自动序列消除了手动样本的变化，并导致更有效地利用光束时间。