DECTECTION OF EARLY CANCER USING FUNCTIONAL OPTICAL COHERENCE TOMOGRAPHY

使用功能光学相干断层扫描检测早期癌症

• 批准号: 7954827
• 负责人: ZHONGPING CHEN
• 金额: $ 0.3万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954827
• 关键词: 3-Dimensional; Address; Animal Model; Animals; Antibodies; Benign; Biotechnology; Caliber; Cancer Detection; Cancer Diagnostics; Cancer Etiology; Categories; Cessation of life; Chest; Clinical; Clinical Research; Clinical Trials; Computer Retrieval of Information on Scientific Projects Database; Contrast Media; Development; Diagnosis; Diagnostic; Diagnostic Imaging; Discrimination; Early Diagnosis; Endoscopy; Funding; Future; Gold; Grant; Granulation Tissue; Granuloma; Histologic; Histology; Human; Image; Imagery; Institution; Lasers; Liposomes; Malignant - descriptor; Malignant Neoplasms; Malignant neoplasm of lung; Metaplasia; Methods; Neoplasms; Optical Coherence Tomography; Optics; Pathologic; Patients; Penetration; Phase; Pleural; Pleural Neoplasms; Research; Research Personnel; Resolution; Resources; Sensitivity and Specificity; Source; Specimen; Speed; Staging; Surface; Techniques; Technology; Testing; Time; Tissues; Translating; United States; United States National Institutes of Health; Woman; analytical method; base; design; flexibility; imaging modality; improved; in vivo; malignant mouth neoplasm; men; multi-photon; multimodality; nanoparticle; optical imaging; pre-clinical

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Lung cancer is by far the leading cause of cancer death in both men and women in the United States (1-3). Successful treatment of lung cancer requires early diagnosis. However, the vast majority of lung cancer is diagnosed at late stage when survival is poor. Improved methods for imaging and diagnosing lung cancers are needed. With improvements in resolution, contrast and probes, optical coherence tomography (OCT) offers the potential to help achieve this objective. We propose that in the future multimodality optical imaging, combining autofluorescence, ultrahigh resolution OCT, and multi-photon/confocal endoscopy may ultimately provide a fully effective approach to this problem. This proposal addresses the initial phases of this concept: development and optimization of OCT for diagnosis of early airway cancer. We propose that high resolution, in-vivo thoracic endoscopic three dimensional (3-D) OCT can help diagnose malignancy-related surface and subsurface morphological and structural changes in order to advance bronchoscopic airway cancer diagnostic capabilities. The objectives of the proposed research are to: 1) develop real-time high speed flexible fiberoptic and rigid endoscopic techniques for 3-dimensional near histologic-resolution optical visualization of airway and pleural malignant pathophysiologic changes, 2) enhance optical contrast to improve diagnostic imaging discrimination, 3) translate and optimize this technology in animal models, and 4) apply endoscopic OCT technology in-vivo in clinical studies to demonstrate feasibility for improving sensitivity and specificity of diagnosis and treatment in patients with bronchogenic airway cancers. The specific aims of this proposal are to: 1. Develop, integrate, and utilize i) small diameter flexible fiberoptic 3-D bronchoscopic MEMS based, and ii) rigid endoscopic 3-D OCT probes. 2. Develop and test antibody directed gold nano-particle liposomal OCT tissue contrast enhancement methods. Image normal and malignant excised animal model tissue with OCT, enhanced tissue contrast agent OCT, and compare structural images to histology. 3. Evaluate F-OCT endoscopy designs and contrast enhancement methods using oral cancer and pleural tumor animal models in initial pre-clinical animal studies to advance technical delivery, contrast, and analytical methods for subsequent application to clinical airway malignancy, 4. Image human airway and pleural specimens with the following categories of presentations: 1- normals, 2  metaplasia, 3  malignant neoplasia, and 4- benign "negative control" airway abnormalities including granulomas, strictures, and granulation tissue to determine capabilities of 3-D F-OCT diagnostic parameters of resolution, depth of penetration, and ability to differentiate pathologic abnormalities. 5. Perform clinical investigations to demonstrate high resolution endoscopic F-OCT feasibility for early endobronchial cancer detection and the ability to diagnose endobronchial cancer with improved specificity and sensitivity compared to histology.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 到目前为止，肺癌是美国男性和女性癌症死亡的主要原因（1-3）。成功治疗肺癌需要早期诊断。 但是，绝大多数肺癌在生存差的后期被诊断出。 需要改进成像和诊断肺癌的方法。 随着分辨率，对比度和探针的改善，光学相干断层扫描（OCT）提供了帮助实现这一目标的潜力。 我们建议，在将来的多模式光学成像中，结合自动荧光，超高分辨率OCT和多光子/共聚焦内窥镜检查最终可能为此问题提供完全有效的方法。 该提议探讨了此概念的初始阶段：诊断早期气道癌症的OCT的开发和优化。 我们建议高分辨率，体内胸腔内镜三维（3-D）OCT可以帮助诊断与恶性肿瘤相关的表面和地下形态和结构变化，以提高支气管镜呼吸道癌症诊断能力。 拟议的研究的目标是：1）开发实时高速柔性光纤和刚性内窥镜技术，用于三维近组织学分辨率的近组织学 - 气道和胸膜恶性病理生理学变化的光学可视化，2）歧视，3）在动物模型中翻译和优化这项技术，4）在临床研究中采用内窥镜OCT技术在临床研究中，以证明可行性可以提高支气管气道癌症患者的诊断和治疗的敏感性和特异性。 该提案的具体目的是： 1。开发，整合和利用i）基于基于支气管镜的小直径柔性3-D支气管镜MEMS，ii）刚性内镜3-D OCT探针。 2。开发和测试抗体定向金纳米颗粒脂质体OCT组织对比度增强方法。 图像正常和恶性切除的动物模型组织具有OCT，增强的组织对比剂OCT，并将结构图像与组织学比较。 3。在最初的临床前动物研究中，使用口腔癌和胸膜肿瘤动物模型评估F-OCT内窥镜设计和对比度增强方法，以推动技术传递，对比度和分析方法，以随后应用于临床气道恶性肿瘤， 4。图像人类气道和胸膜标本，具有以下演示类别的类别：1-正常，2个Metaplasia，3种恶性肿瘤和4-良性“阴性对照”气道异常，包括颗粒，严格和颗粒状组织，以确定3-D F-OCT分辨率，穿透深度和区分病理异常的能力的3-D F-OCT诊断参数。 5。进行临床研究，以证明高分辨率内窥镜F-OCT可行性，可与组织学相比，对早期内离癌症检测的可行性以及具有提高的特异性和敏感性的诊断具有提高特异性和敏感性的能力。