The function of small RNAs in the nitrogen response

小RNA在氮反应中的功能

基本信息

批准号：
7746444
负责人：
Gloria CORUZZI
金额：
$ 2.54万
依托单位：
NEW YORK UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-01-01 至 2010-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7746444
关键词：
Address Affect Agriculture Amino Acids Animals Arabidopsis Area Assimilations Bioinformatics Biology Carbon Cells Chile Collaborations Computers DNA Sequence Data Set Development Dose Ectopic Expression Experimental Designs Frequencies Funding Future Gene Expression Gene Expression Regulation Gene Targeting Genes Genetic Screening Genome Genomics Goals Grant Growth and Development function Health Human Imagery Individual Letters Light Logic Mediating Mentors Metabolic MicroRNAs Microarray Analysis Modeling Molecular Nitrogen Organism Parents Pathway interactions Plants Play Positioning Attribute Post-Transcriptional Regulation Principal Investigator Process Regulation Research Research Personnel Role Sampling Seeds Signal Transduction Small RNA Source South America Students System Technology Testing Time Training Transcript Travel United States National Institutes of Health Universities Work Writing aminoacid biosynthesis base combinatorial design improved in vivo insight metabolomics mutant network models novel nutrition positional cloning professor programs research study response tool

项目摘要

DESCRIPTION (provided by applicant): Our long-term goal is to understand how nitrogen (N) signaling controls plant N-assimilation, growth and development. Identifying key components involved in regulating N-assimilation offers opportunities for improving N-use efficiency in plants or modifying the amino acid content of seeds, which are important issues for health, agriculture and human nutrition. The experiments in this proposal should provide insight into how plants sense and respond to N at the molecular level, specifically through the post transcriptional control of gene expression by microRNAs (miRNAs) and other small RNAs (sRNAs). Identifying the sRNAs involved in regulation of gene networks in response to N-treatment could identify mechanisms which may be altered to optimize N-assimilation via the ectopic expression or misexpression of specific sRNAs. It is now clear that miRNAs are key components of regulatory networks in plants and animals, but their role in mediating the N- response of target genes has not been addressed. We propose to (1) Identify the miRNAs and other sRNAs whose expression is regulated by N-treatments; (2) Identify the gene networks that are controlled by these N- regulated sRNAs; and (3) Understand the functional role of sRNAs in mediating the N-response of target genes in the network. Our experimental approach will be to isolate sRNAs from plants treated transiently with inorganic N sources, sequence the sRNAs using 454 technology, and compare the frequency of transcripts in these samples with the frequency in samples from mock-treated plants. We will incorporate known and novel sRNA:RNA interactions into our existing gene network models to identify subnetworks that are controlled by N- responsive sRNAs. We will use reverse genetic and genomic approaches to evaluate the role of specific sRNAs in the plant response to N-treatments. The combination of bioinformatic and genomic approaches for functional studies in this project performed in collaboration with Dr. Coruzzi's group, will greatly strengthen the research capability of Dr. Gutiirrez research program in Chile and South America. Expertise in these areas is essential for competitive research programs in the post-genomic era, and it is an underdeveloped area of research in Chile and South America. Most of the research will be carried out in Chile at P. Universidad Catslica de Chile in collaboration with Dr. Rodrigo Gutiirrez, as an extension of NIH grant #2R01GM032877- 21 to Dr. Gloria M. Coruzzi. This proposal should provide insight into how plants sense and respond to nitrogen at the molecular level. Identifying key components involved in regulating nitrogen assimilation offers opportunities for improving nitrogen-use efficiency in plants or modifying the amino acid content of seeds, which are important issues for health, agriculture and human nutrition.

描述（由申请人提供）：我们的长期目标是了解氮（N）信号如何控制植物N-鉴别，生长和发育。确定调节N-综合涉及的关键组成部分为提高植物的N使用效率或修改种子的氨基酸含量提供了机会，这是健康，农业和人类营养的重要问题。该提案中的实验应提供有关植物在分子水平上的感知和响应的见解，特别是通过microRNA（miRNA）和其他小RNA（SRNA）对基因表达的转录后控制。识别响应N处理中涉及的基因网络调节的SRNA可以识别可以通过异位表达或特定SRNA的Misexpression进行优化的机制，以优化N-识别。现在很明显，miRNA是动植物中调节网络的关键组成部分，但是它们在介导靶基因的N响应中的作用尚未得到解决。我们建议（1）确定由N-处理调节的miRNA和其他SRNA；（2）确定由这些N-调控SRNA控制的基因网络；（3）了解SRNA在介导网络中靶基因的N反应中的功能作用。我们的实验方法是将SRNA与用无机N来源瞬时处理的植物分离，使用454技术对SRNA进行序列，并比较这些样品中的转录本的频率与模拟植物的样品中的频率。我们将将已知和新颖的SRNA：RNA相互作用纳入我们现有的基因网络模型中，以识别由n响应式SRNA控制的子网。我们将使用反向遗传和基因组方法来评估特定SRNA在植物对N治疗反应中的作用。该项目与Coruzzi博士小组合作进行的该项目中的生物信息学方法和基因组方法的结合将极大地增强智利和南美洲Gutiirrez研究计划的研究能力。这些领域的专业知识对于后代后时代的竞争性研究计划至关重要，它是智利和南美的研究领域欠发达的领域。大多数研究将与智利大学智利智利智利进行，与Rodrigo Gutiirrez博士合作，作为NIH Grant＃2R01GM032877-21的扩展，向Gloria M. Coruzzi博士致敬。该提案应洞悉植物在分子水平上如何对氮的感觉和反应。确定调节氮同化的关键组成部分为提高植物中氮的效率或修饰种子的氨基酸含量提供了机会，这对于健康，农业和人类营养而言是重要的问题。