Resonance Aided Insertion Lancet for serial blood sampling in age-related studies

共振辅助插入柳叶刀用于年龄相关研究中的连续血液采样

• 批准号: 7999435
• 负责人: Ryan S Clement
• 金额: $ 15.67万
• 依托单位: ACTUATED MEDICAL, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2012-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7999435
• 关键词: Age; Aging; Aging-Related Process; Alzheimer' s Disease; Animal Testing; Animals; Antibodies; Area; Automobile Driving; Beginning of Life; Behavior; Behavioral; Biochemical; Biological; Biological Assay; Biological Markers; Blood Chemical Analysis; Blood specimen; Cancer Immunology Science; Cells; Corticosterone; Cross-Sectional Studies; Development; Devices; Disease; Ensure; Exhibits; Frequencies; Generations; Goals; Hand; Heating; Histology; Hormones; In Situ; Individual; Kinetics; Lead; Link; Longevity; Longitudinal Studies; Manuals; Medical; Medical Device; Metabolism; Needles; Neurobiology; Pain; Parkinson Disease; Performance; Phase; Physiological; Pilot Projects; Play; Procedures; Production; Proteins; Psyche structure; Puncture procedure; Rattus; Research; Research Design; Research Personnel; Resort; Risk Management; Rodent; Rodent Model; Role; Safety; Sampling; Sampling Studies; Small Business Innovation Research Grant; Solutions; Stress; Tail; Techniques; Technology; Temperature; Testing; Tissues; Tube; United States National Institutes of Health; Veins; Venipunctures; Work; age difference; age related; design; experience; improved; innovation; insight; nutrition; operation; programs; prototype; public health relevance; research and development; tool; vibration

DESCRIPTION (provided by applicant): Rodent studies have provided significant insights into the general aging process and age-related topics such as nutrition, behavior, immunology, cancer, and neurobiology. In such studies, concentrations of hormones, proteins, antibodies, and other metabolites and their associated kinetics are popular biomarkers because they vary with age and disease state. Typically biochemical assays are performed on blood samples collected following the temporary puncture of a large target vein. Standard blood sampling procedures can increase stress hormone release which may confound blood chemistry analysis, as well as cause pain and discomfort. This hormone release is especially problematic when serial samples are to be obtained over the lifespan. Minimally- perturbing blood sampling techniques are needed that enable studies of hormone fluctuations over the lifespan of individual animals. In this NIH SBIR program, Piezo Resonance Innovations Inc. (PRII) will demonstrate the feasibility of the Resonance Aided Insertion Lancet, an active device specifically designed to reduce the insertion forces required to perform tail venipuncture. In a pilot study conducted by PRII, a prototype was used to obtain blood samples from the tail vein of rats. The study suggested that blood samples were obtained with less stress according to behavioral indicators and corticosterone levels, compared to that of conventional manual lancet insertion. The goals of the Phase I proposal is to determine the parameters for the optimal prototype design, and conduct a more comprehensive set of bench and animal tests to ensure safety and efficacy. The proposed work will lead to the development of a new tool that minimizes animal stress during serial blood samples reducing the number of animals needed per investigational study - animal can survive entire study. This results in fewer animals needed per study, and improved humane treatment of the animals. PUBLIC HEALTH RELEVANCE: Understanding the physiological and biological mechanisms of aging and the role hormones play in healthy and abnormal aging continues to be the focus of much research. Studies of hormone levels in rodent models have been critical to this effort, but the effect of the stress and pain associated with traditional blood sampling techniques is problematic. New minimally-perturbing blood sampling strategies, such as the device to be developed here, are highly desired to enable serial blood sampling over the lifespan to better explore links between changing hormone levels and the aging process.

描述（由申请人提供）：啮齿动物研究为一般衰老过程和与年龄相关的主题（如营养、行为、免疫学、癌症和神经生物学）提供了重要的见解。在此类研究中，激素、蛋白质、抗体和其他代谢物的浓度及其相关动力学是流行的生物标志物，因为它们随着年龄和疾病状态而变化。通常，对大目标静脉临时穿刺后收集的血液样本进行生化测定。标准血液采样程序会增加应激激素的释放，这可能会干扰血液化学分析，并引起疼痛和不适。当要在整个生命周期中获取连续样本时，这种激素释放尤其成问题。需要最小扰动的血液采样技术来研究个体动物一生中的激素波动。在这个 NIH SBIR 项目中，压电共振创新公司 (PRII) 将展示共振辅助插入刺血针的可行性，这是一种专门设计用于减少尾部静脉穿刺所需的插入力的主动装置。在 PRII 进行的一项试点研究中，使用原型从大鼠尾静脉获取血液样本。研究表明，与传统的手动刺血针插入相比，根据行为指标和皮质酮水平，获得血液样本的压力更小。第一阶段提案的目标是确定最佳原型设计的参数，并进行更全面的台架和动物测试，以确保安全性和有效性。拟议的工作将导致开发一种新工具，最大限度地减少连续血液样本期间动物的压力，从而减少每个研究所需的动物数量——动物可以在整个研究中存活下来。这导致每项研究所需的动物更少，并改善了对动物的人道待遇。 公共健康相关性：了解衰老的生理和生物学机制以及激素在健康和异常衰老中的作用仍然是许多研究的重点。对啮齿动物模型中激素水平的研究对于这项工作至关重要，但与传统血液采样技术相关的压力和疼痛的影响是有问题的。非常需要新的最小干扰血液采样策略，例如这里开发的设备，能够在整个生命周期内进行连续血液采样，以更好地探索激素水平变化与衰老过程之间的联系。

项目成果

Effects of Axial Vibration on Needle Insertion into the Tail Veins of Rats and Subsequent Serial Blood Corticosterone Levels.

轴向振动对大鼠尾静脉针插入及随后连续血皮质酮水平的影响。

• 发表时间: 2016
• 期刊: Journal of the American Association for Laboratory Animal Science : JAALAS
• 作者: Clement,RyanS;Unger,EricaL;Ocón-Grove,OlgaM;Cronin,ThomasL;Mulvihill,MaureenL
• 通讯作者: Mulvihill,MaureenL