Molecular Mechanisms of miRNA Function

miRNA功能的分子机制

• 批准号: 7615409
• 负责人: CATHERINE A REINKE
• 金额: $ 5.34万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7615409
• 关键词: Alleles; Biochemical; Biological Process; Complex; Defect; Development; Disease; Drosophila genus; Drosophila melanogaster; Evaluation; Gene Expression; Gene Expression Regulation; Generations; Genes; Genetic Screening; Human; Investigation; Libraries; Malignant Neoplasms; Mediating; MicroRNAs; Molecular; Pathway interactions; Phenotype; Proteins; Public Health; RNA; RNA Interference; Reporter; Role; Therapeutic; Transgenic Organisms; base; combat; gene function; human DICER1 protein; mutant; novel; positional cloning; sensor; tumor

DESCRIPTION (provided by applicant): These investigations seek to identify the molecular mechanisms of microRNA-mediated gene regulation. The isolation and characterization of Drosophila melanogaster mutants will be carried out to identify genes that function in the miRNA pathway and to elucidate the mechanism by which miRNA effector complexes act on target mRNAs. miRNA function impacts public health, given that many human tumors show altered miRNA profiles. In addition, the responsible use of RNA-based therapeutics requires an understanding of miRNA function, given the observation of crosstalk between RNA-mediated silencing pathways. 1. To isolate Drosophila melanogaster mutants with disrupted miRNA-mediated gene regulation 1.1 Generate EMS-mutagenized Drosophila lines with altered expression of a sensor of miRNA function 1.2 Conduct a pilot reverse genetic screen to identify transgeneic lines in the Drosophila RNAi library with altered expression of a sensor of miRNA function 2. To prioritize candidate mutant lines for further study by characterizing additional phenotypic defects 2.1 Analyze candidate lines using alternate sensors of miRNA function to determine whether mutant lines demonstrate general or reporter-specific effects 2.2 Classify candidate lines by comparing the phenotypes of newly isolated mutants to the characterized phenotypes (including developmental and RNAi defects) of known miRNA pathway mutants 3. To identify genes in the miRNA pathway and assess their role in generating miRNA effector complexes 3.1 Characterize alleles of known miRNA pathway genes Dicer-1 and Pasha 3.2 Determine the identities of 2-3 novel genes in the miRNA pathway 3.3 Perform a biochemical evaluation of miRNA complexes to characterize miRNA pathway genes Precise gene regulation is essential to normal biological function. An understanding the newly discovered roles of RNA in gene regulation is essential to our potential ability to combat many gene regulation diseases, most notably cancer.

描述（由申请人提供）：这些研究旨在确定microRNA介导的基因调节的分子机制。将进行果蝇的分离和表征，以识别在miRNA途径中起作用的基因，并阐明miRNA效应复合物对靶mRNA作用的机制。鉴于许多人类肿瘤表现出改变的miRNA特征，miRNA功能会影响公共卫生。此外，考虑到RNA介导的沉默途径之间的串扰，负责使用基于RNA的治疗剂需要了解miRNA功能。 1。要分离果蝇黑色素持突变体与中断的miRNA介导的基因调控1.1产生EMS-核酸果仁果果蝇线，miRNA函数传感器的表达改变了miRNA功能的表达1.2进行飞行员反向遗传筛查，以鉴定果蝇库中的经跨性别线的传播线，并鉴定出mirna rnai库的传感器表达。 2。通过表征其他表型缺陷来优先考虑候选突变线，使用miRNA功能的替代传感器分析候选线2.1分析候选线，以确定突变线是否证明了一般或报告特异性效应2.2分类候选候选线，通过比较新分离的突变体的表型与新的表征性现象（包括发育式和RNAI Defects of Newai searie Defects of Newally Syly Simant）。 3。要鉴定miRNA途径中的基因并评估其在产生miRNA效应复合物复合物中的作用3.1表征已知miRNA途径基因dicer-1和pasha 3.2的等位基因3.2确定miRNA途径中2-3个新型基因的身份3.3在3.3中执行mirna pathway基因的生物化学评估 精确的基因调节对于正常的生物学功能至关重要。理解RNA在基因调节中的新作用对于我们对抗许多基因调节疾病的潜在能力至关重要，最著名的是癌症。