CT IMAGING OF BLOOD VESSEL IN TRANSGENIC MOUSE MODELS FOR HUMAN TUMORS

人类肿瘤转基因小鼠模型中血管的 CT 成像

• 批准号: 7723096
• 负责人: CHRISTOPHER R. JOHNSON
• 金额: $ 4.62万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723096
• 关键词: Animals; Blood; Blood Vessels; Blood Volume; Caliber; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Contrast Media; Environment; Funding; Grant; Guidelines; Human; Image; Imagery; Immune system; In Situ; Institution; Life; Magnetic Resonance Imaging; Malignant Neoplasms; Methods; Mus; Research; Research Personnel; Resources; Scanning; Source; Therapy Clinical Trials; Time; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Visual; X-Ray Computed Tomography; Xenograft Model; angiogenesis; density; human cancer mouse model; instrument; mouse model; pre-clinical; tool; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transgenic mouse models of human cancer have the potential to be more reflective of human cancers than xenograft models because transgenic mice form tumor in situ, i.e. in an environment more similar to the human tumor and in the setting of a normal immune system. Small animal X-ray computed tomography (microCT) is an economical and highly quantitative three-dimensional method for visualizing blood vessels and angiogenesis preclinically, even in comparison to small animal magnetic resonance imaging. In this collaboration, we are developing practical guidelines for rapid, accurate visualization of intermediate to large caliber (greater than 93 micron) blood vessels for serial assessment of vascularity during preclinical therapeutic trials in living mice. Because of the long scan times for most small animal computed tomography instruments, we are using a long-acting blood pool contrast agent. In addition to guidelines, we are also further developing tools to assess vessels through qualitative visual renderings. The same optimized acquisition settings will be necessary for segmentation analysis and will allow quantitative analysis of tumor blood volume, vessel density, vessel caliber, degree of branching, and tortuosity.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 人类癌症的转基因小鼠模型有可能比异种移植物更反映人类癌症 模型是因为转基因小鼠原位形成肿瘤，即在与人类肿瘤更相似的环境中 正常免疫系统的设置。小动物X射线计算机断层扫描（Microct）是一种经济的且高度 定量的三维方法，用于促进血管和血管生成，即使与 小动物磁共振成像。在这项合作中，我们正在制定实用准则，以快速，准确 可视化中间至大口径（大于93微米）的血管，用于血管串行评估 在活小鼠的临床前治疗试验中。由于大多数计算的小动物的扫描时间很长 断层扫描仪器，我们正在使用长效的血池对比剂。除了准则，我们也是 进一步开发通过定性视觉渲染评估船舶的工具。 相同的优化收购设置 分割分析是必要的，并且可以定量分析肿瘤血容量，血管密度， 容器的口径，分支程度和曲折。