Mechanism of Sparse Precise Bursting in the Nucleus HVC

HVC 核稀疏精确爆发机制

基本信息

批准号：
7211384
负责人：
HENRY S GREENSIDE
金额：
$ 23.14万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-06-01 至 2009-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7211384
关键词：
Acoustics Action Potentials Address Adolescent Adult Affect Anatomy Anterior Area Auditory Axon Bathing Behavior Birds Brain Cell Nucleus Cells Chiroptera Chromosome Pairing Class Collaborations Complement Condition Coupled Coupling Crystallization Data Data Analyses Dependence Depth Dyes Electrodes Employee Strikes Excitatory Synapse Exposure to Feedback Figs - dietary Fire - disasters Frequencies Funding Agency Future GABA Receptor Gap Junctions Generations Glutamates Goals Grant Head Human Implant In Vitro Inhibitory Synapse Injection of therapeutic agent Interneurons Ion Channel Joints K-Series Research Career Programs Knowledge Label Language Learning Left Lesion Letters Maintenance Mammals Measurement Measures Membrane Memory Mentors Modeling Motor Muscle Muscle Tension N-Methylaspartate Neurobiology Neurons Neurosciences Noise Nonlinear Dynamics Numbers Optics Other Finding Output Paper Partner in relationship Pathway interactions Phase Physics Physiologic pulse Physiological Physiology Plastics Play Population Postdoctoral Fellow Principal Investigator Probability Production Property Prosencephalon Pulse taking Range Rate Research Research Personnel Resolution Respiration Role Sensory Series Signal Transduction Slice Songbirds Speech Speech Pathology Staging Students Study models Stuttering Synapses Synaptic Transmission Techniques Testing Theoretical model Thinking Time Tissues Training Ultrasonography United States National Institutes of Health Variant Work auditory feedback auditory nuclei awake bird song concept day excitatory neuron experience extracellular feeding improved in vivo inhibitory neuron interest male mathematical model member millisecond postsynaptic pressure professor receptor research study respiratory response simulation sound statistics theories transmission process vocal learning voltage voltage clamp zebra finch

项目摘要

DESCRIPTION (provided by applicant): The goal for this revised two-year K25 Mentored Quantitative Research Career Development Award is for the PI to gain enough knowledge and research experience in neurobiology to make a transition from research in theoretical and computational physics to research in theoretical I neurobiology, especially as related to auditory-guided vocal learning of songbirds. Songbird research is important for the long-term goals of NIDCD because songbirds provide one of the few experimental paradigms to understand at the cellular and circuit levels how humans learn to speak and how speech pathologies may arise and be treated. Experiments have shown that the songbird telencephalic nucleus HVC is critical for song learning and song production. An intriguing property of HVC projection neurons is that they produce temporally sparse bursts of action potentials during singing and during song playback, and these bursts are thought to be important for auditory and motor encoding of the song. Under the guidance of mentor Professor Richard Mooney and over an 18-month period, the PI will learn experimental techniques and carry out three projects in the Mooney lab to clarify the mechanism and significance of the sparse bursts: single-electrode intracellular recordings in HVC slices to characterize the intrinsic dynamical properties of pharmacologically isolated HVC neurons; dual intracellular recordings of neurons that have been verified to be synaptically connected, to study how the sparse bursts of HVC-to-RA projection neurons influence the tonic firing of HVC interneurons; and dual-intracellular recordings combined with antidromic stimulation of HVC-to-RA projection neurons, to quantify the degree of convergence of projection neurons onto interneurons. The PI will use the experimental data obtained from these projects, together with a deeper understanding of theoretical neurobiology obtained during the K25, to develop and to improve mathematical models of how sparse preciselyaligned bursts arise in HVC, and especially to test the hypothesis that the bursts during singing are produced by an intrinsic causal mechanism such as a synfire chain.

描述（由申请人提供）：修订后的两年期 K25 指导定量研究职业发展奖的目标是让 PI 获得足够的神经生物学知识和研究经验，以从理论和计算物理研究过渡到理论和计算物理研究我是神经生物学，特别是与鸣禽的听觉引导发声学习有关的神经生物学。鸣禽研究对于 NIDCD 的长期目标非常重要，因为鸣禽提供了为数不多的实验范式之一，可以在细胞和电路水平上了解人类如何学习说话以及言语病理如何出现和治疗。实验表明，鸣禽端脑核 HVC 对于鸣叫学习和鸣叫产生至关重要。 HVC 投射神经元的一个有趣特性是，它们在唱歌和歌曲播放期间产生暂时稀疏的动作电位突发，并且这些突发被认为对于歌曲的听觉和运动编码很重要。在导师 Richard Mooney 教授的指导下，在 18 个月的时间里，PI 将学习实验技术并在 Mooney 实验室开展三个项目，以阐明稀疏爆发的机制和意义：HVC 切片中的单电极细胞内记录表征药理学分离的 HVC 神经元的内在动力学特性；已证实具有突触连接的神经元的双重细胞内记录，以研究 HVC 到 RA 投射神经元的稀疏爆发如何影响 HVC 中间神经元的强直放电；以及双细胞内记录与 HVC-to-RA 投射神经元的逆向刺激相结合，以量化投射神经元到中间神经元的收敛程度。 PI 将利用从这些项目中获得的实验数据，以及 K25 期间获得的对理论神经生物学的更深入理解，开发和改进 HVC 中稀疏精确对齐突发如何出现的数学模型，特别是测试突发的假设歌唱期间的声音是由内在的因果机制产生的，例如合成火链。