Are anthocyanins necessary for oral cancer chemoprevention by berries?

浆果化学预防口腔癌是否需要花青素?

基本信息

  • 批准号:
    7590645
  • 负责人:
  • 金额:
    $ 7.5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-09-12 至 2010-08-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Numerous epidemiological studies have shown a strong correlation between consumption of fresh fruits and vegetables and a decreased risk of oral cancer. Several preclinical studies demonstrate the remarkable chemopreventive activity of two berry fruits, black raspberries and strawberries, that inhibit human oral cancer cell proliferation in vitro and development of chemically-induced aerodigestive tract tumors in animals. A pressing, yet unanswered, question remains "What types of polyphenols in fruit are primarily responsible for these activities?" Prior studies have employed lyophilized powders or extracts of ripe fruits containing a complex mixture of berry polyphenols of which a major group is the anthocyanins. Epidemiological evidence suggests that diets rich in anthocyanins (colored fruits and vegetables) reduce the overall risk of cancer and laboratory evidence illustrates the inhibition of proliferation of tumor cells in culture and prevention of tumors in animal models. However, there are numerous other fruit phenolics in berries that have demonstrated antioxidant, antiproliferative, and chemopreventive activity in both cell cultures and animal models of cancer. Among these are the phenolic acids, (gallic, procatechuic, p-hydroxybenzoic, caffeic, p- coumaric, ferulic, chlorogenic, and ellagic acids), the flavonols (quercetin, kaempferol, mycetrin, and isorhamntin), and complex polyphenols (lignans, ellagitannin, and gallotannin). To examine the chemopreventive capacity of these latter berry phenolics, separate from their combined activity with anthocyanins, we propose to evaluate green berry fruits (with minimal anthocyanin content) and ripe berry fruits (with high levels of anthocyanins) for inhibition of oral tumorigenesis in an animal model and to determine their effect on modulation of cancer-associated genes. Hypothesis: We hypothesize that bioactive phenolics found in green strawberries and black raspberries, independent of the anthocyanins, will inhibit tumorigenesis just as effectively as ripe berries in an animal model of oral cancer and will modulate the expression of critical genes associated with oral cancer. Methods: Hamster pellets will be prepared using 5% and 10% green and ripe strawberries and black raspberries. Beginning 7 days after commencement of feeding with berry pellets, cheek pouches will be painted 3x/week for six weeks with the chemical carcinogen 7,12-dimethylbenz(a)anthracene. Berry pellets will be given during, and for six weeks after, carcinogen treatment. At 12-13 weeks, cheek pouches will be evaluated for total lesions and tumors and for modulation of caner- associated gene expression. Significance: The novel studies outlined herein will provide a practical assessment of the chemopreventive potential of berries that are essentially devoid of anthocyanins, as well as an opportunity to determine their ability to modulate specific molecular targets in oral cancers. The outcome will provide a suitable foundation for in-depth studies designed to elucidate the chemoprevention potential for oral cancer of various bioactive berry components.
描述(由申请人提供): 许多流行病学研究表明,新鲜水果和蔬菜的消费与口腔癌的风险降低之间存在很强的相关性。几项临床前研究表明,两种浆果水果,黑色覆盆子和草莓具有显着的化学预防活性,可抑制人类口腔癌细胞的体外口腔癌细胞增殖,并发展动物中化学诱导的气化剂肿瘤。一个紧迫但没有解决的问题仍然存在:“水果中哪些类型的多酚主要负责这些活动?”先前的研究采用了冻干粉末或成熟水果提取物,这些果实含有浆果多酚的复杂混合物,其中主要基团是花青素。流行病学证据表明,富含花青素的饮食(有色水果和蔬菜)降低了癌症和实验室证据的总体风险,这表明抑制了动物模型中肿瘤细胞在培养和预防肿瘤中的增殖。但是,浆果中还有许多其他水果酚类在细胞培养物和癌症的动物模型中都表现出抗氧化,抗增生性和化学预防活性。 Among these are the phenolic acids, (gallic, procatechuic, p-hydroxybenzoic, caffeic, p- coumaric, ferulic, chlorogenic, and ellagic acids), the flavonols (quercetin, kaempferol, mycetrin, and isorhamntin), and complex polyphenols (lignans, ellagitannin, and Gallotannin)。为了检查这些后一种浆果酚类的化学预防能力,与它们与花青素的合并活性分开,我们建议评估绿色浆果水果(具有最小的花青素含量)和成熟的浆果水果(具有高水平的花青素),以抑制其在动物模型中的口腔肿瘤,以抑制其在动物模型中的癌症效应。假设:我们假设在绿色草莓和黑色覆盆子中发现的生物活性酚类(独立于花青素)将与口腔癌模型中的成熟浆果一样有效地抑制肿瘤发生,并将调节与口腔癌相关的关键基因的表达。方法:将使用5%和10%绿色和成熟的草莓以及黑色覆盆子制备仓鼠颗粒。从浆果颗粒开始喂食后7天,将用化学致癌物7,12-二甲基苯子(A)蒽(a)将脸颊袋涂成3倍/周六周。浆果颗粒将在致癌物治疗期间和六个星期内给予。在12-13周时,将评估脸颊袋的总病变和肿瘤,并调节罐头相关的基因表达。意义:本文概述的新研究将对基本上没有花色苷的浆果的化学预防潜力进行实际评估,以及确定其调节口服癌症特定分子靶标的能力的机会。该结果将为深入研究提供合适的基础,旨在阐明各种生物活性浆果成分口腔癌的化学预防潜力。

项目成果

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CHRISTOPHER M WEGHORST其他文献

CHRISTOPHER M WEGHORST的其他文献

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{{ truncateString('CHRISTOPHER M WEGHORST', 18)}}的其他基金

Inhibition of Rat Oral Carcinogenesis by Dietary Black Raspberries
食用黑树莓对大鼠口腔癌的抑制作用
  • 批准号:
    8492247
  • 财政年份:
    2013
  • 资助金额:
    $ 7.5万
  • 项目类别:
Inhibition of Rat Oral Carcinogenesis by Dietary Black Raspberries
食用黑树莓对大鼠口腔癌的抑制作用
  • 批准号:
    8633444
  • 财政年份:
    2013
  • 资助金额:
    $ 7.5万
  • 项目类别:
Are anthocyanins necessary for oral cancer chemoprevention by berries?
浆果化学预防口腔癌是否需要花青素?
  • 批准号:
    7686731
  • 财政年份:
    2008
  • 资助金额:
    $ 7.5万
  • 项目类别:
Food-Based Modulation of Biomarkers in Human Tissues at High-Risk for Oral Cancer
基于食物的口腔癌高风险人体组织中生物标志物的调节
  • 批准号:
    7370901
  • 财政年份:
    2007
  • 资助金额:
    $ 7.5万
  • 项目类别:
Food-Based Modulation of Biomarkers in Human Tissues at High-Risk for Oral Cancer
基于食物的口腔癌高风险人体组织中生物标志物的调节
  • 批准号:
    7538414
  • 财政年份:
    2007
  • 资助金额:
    $ 7.5万
  • 项目类别:
Food-Based Modulation of Biomarkers in Human Tissues at High-Risk for Oral Cancer
基于食物的口腔癌高风险人体组织中生物标志物的调节
  • 批准号:
    8197486
  • 财政年份:
    2007
  • 资助金额:
    $ 7.5万
  • 项目类别:
Prevention of Oral Cancer by Strawberries with Selenium Assimilated in the Fruit
草莓中吸收的硒可预防口腔癌
  • 批准号:
    7392146
  • 财政年份:
    2007
  • 资助金额:
    $ 7.5万
  • 项目类别:
Prevention of Oral Cancer by Strawberries with Selenium Assimilated in the Fruit
草莓中吸收的硒可预防口腔癌
  • 批准号:
    7264122
  • 财政年份:
    2007
  • 资助金额:
    $ 7.5万
  • 项目类别:
Food-Based Modulation of Biomarkers in Human Tissues at High-Risk for Oral Cancer
基于食物的口腔癌高风险人体组织中生物标志物的调节
  • 批准号:
    8007435
  • 财政年份:
    2007
  • 资助金额:
    $ 7.5万
  • 项目类别:
Food-Based Modulation of Biomarkers in Human Tissues at High-Risk for Oral Cancer
基于食物的口腔癌高风险人体组织中生物标志物的调节
  • 批准号:
    7743785
  • 财政年份:
    2007
  • 资助金额:
    $ 7.5万
  • 项目类别:

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