Detection of in vivo protease activities using PARACEST MRI contrast agents

使用 PARACEST MRI 造影剂检测体内蛋白酶活性

• 批准号: 7451742
• 负责人: Mark David Pagel
• 金额: $ 20.39万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7451742
• 关键词: Accounting; Achievement; Address; Animal Model; Animals; Apoptosis; Biochemical; Biological Assay; Biological Markers; Biological Monitoring; Biological Process; Breast Carcinoma; Catalysis; Cathepsin L; Cathepsins B; Cell Death; Cells; Chemicals; Cleaved cell; Clinical; Clinical Pathology; Condition; Contrast Media; Coupled; Cultured Cells; Cytosol; Detection; Development; Diagnostic; Drug Kinetics; Endopeptidases; Enzymes; Evolution; Foundations; Goals; Human; Human Mammary Carcinoma; Hydrogen; In Situ Nick-End Labeling; In Vitro; Incubated; Infusion procedures; Invasive; Kinetics; Lesion; MCF7 cell; Magnetic Resonance Imaging; Malignant Neoplasms; Mammary Neoplasms; Methods; Monitor; Neoplasm Metastasis; Normal tissue morphology; Pathology; Peptide Hydrolases; Peptides; Protein Overexpression; Proteins; Rate; Relative (related person); Relaxation; Research; Saline; Sampling; Scanning; Signal Transduction; Solutions; Specificity; Staining method; Stains; TNFSF10 gene; Technology; Therapeutic; Therapeutic Effect; Tissues; Toxic effect; Urokinase; Water; base; caspase-3; design; extracellular; fluorescence imaging; functional group; improved; in vivo; magnetic field; malignant breast neoplasm; molecular imaging; mouse model; neoplastic cell; preclinical study; response; size; subcutaneous; success; tool; tumor

DESCRIPTION (provided by applicant): Proteases are important biomarkers for many pathologies and biological processes and are often targets of chemotherapeutics. Magnetic Resonance Imaging (MRI) is an outstanding diagnostic tool for identifying suspicious lesions, but suffers from many false-positive results due to a lack of specificity for pathological tissues versus normal tissues. The development of MRI contrast agents that can detect protease biomarkers of metastatic tumors or the response to anti-tumor therapies would greatly improve the specificity of MRI for detecting and evaluating cancer. We have developed a fundamentally new type of protease-responsive MRI contrast agent that is detected through PARAmagnetic Chemical Exchange Saturation Transfer (PARACEST). This mechanism allows each PARACEST contrast agent to be selectively detected, so that multiple PARACEST contrast agents may be simultaneously detected during a single MRI scan session, such as a protease-unresponsive PARACEST contrast agent that can serve as a `control' to account for the pharmacokinetics of the agents. Furthermore, we are the first research team to demonstrate the detection of PARACEST contrast agents within in vivo animal models. We will combine our accomplishments with PARACEST contrast agents to detect multiple enzyme-responsive PARACEST contrast agents within in vivo animal models. More specifically, we will design and apply PARACEST contrast agents to detect caspase-3 during TRAIL-induced tumor cell apoptosis in an in vivo mouse model of MCF-7c3 human mammary carcinoma. We will also design and apply PARACEST contrast agents to simultaneously detect cathepsin B and urokinase Plasminogen Activator in an in vivo mouse model of MCF-7 and MBA-MB-231 mammary carcinoma. The successful achievement of our specific aims will result in new platform technology that can be immediately used during many pre-clinical studies, and that can be used as a foundation for further studies to gain clinical approval for this new type of diagnostic molecular imaging agent to detect protease biomarkers in cancer and other pathological tissues. Narrative: A fundamentally new type of protease-responsive MRI contrast agent has been developed that is detected through PARAmagnetic Chemical Exchange Saturation Transfer (PARACEST). Furthermore, PARACEST contrast agents can be detected within in vivo animal models. This research application will combine these accomplishments with PARACEST contrast agents to detect multiple enzyme-responsive PARACEST contrast agents within in vivo animal models.

描述（由申请人提供）：蛋白酶是许多病理学和生物过程的重要生物标志物，并且通常是化疗的靶标。磁共振成像 (MRI) 是识别可疑病变的出色诊断工具，但由于病理组织与正常组织缺乏特异性，因此存在许多假阳性结果。开发能够检测转移性肿瘤的蛋白酶生物标志物或抗肿瘤治疗反应的 MRI 造影剂将大大提高 MRI 检测和评估癌症的特异性。我们开发了一种全新类型的蛋白酶响应 MRI 造影剂，可通过顺磁化学交换饱和转移 (PARACEST) 进行检测。这种机制允许选择性地检测每个 PARACEST 造影剂，以便在单个 MRI 扫描会话期间可以同时检测到多个 PARACEST 造影剂，例如可以充当“对照”以解释蛋白酶无响应的 PARACEST 造影剂。药剂的药代动力学。此外，我们是第一个在体内动物模型中展示 PARACEST 造影剂检测的研究团队。我们将把我们的成就与 PARACEST 造影剂结合起来，在体内动物模型中检测多种酶响应性 PARACEST 造影剂。更具体地说，我们将设计并应用 PARACEST 造影剂来检测 MCF-7c3 人乳腺癌体内小鼠模型中 TRAIL 诱导的肿瘤细胞凋亡过程中的 caspase-3。我们还将设计并应用 PARACEST 造影剂，在 MCF-7 和 MBA-MB-231 乳腺癌体内小鼠模型中同时检测组织蛋白酶 B 和尿激酶纤溶酶原激活剂。我们特定目标的成功实现将产生新的平台技术，该技术可以立即在许多临床前研究中使用，并可以作为进一步研究的基础，以获得这种新型诊断分子成像剂的临床批准检测癌症和其他病理组织中的蛋白酶生物标志物。叙述：已经开发出一种全新类型的蛋白酶响应 MRI 造影剂，可通过顺磁化学交换饱和转移 (PARACEST) 进行检测。此外，可以在体内动物模型中检测到 PARACEST 造影剂。这项研究应用将把这些成就与 PARACEST 造影剂结合起来，在体内动物模型中检测多种酶响应性 PARACEST 造影剂。