Detection of in vivo protease activities using PARACEST MRI contrast agents

使用 PARACEST MRI 造影剂检测体内蛋白酶活性

• 批准号: 7451742
• 负责人: Mark David Pagel
• 金额: $ 20.39万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7451742
• 关键词: Accounting; Achievement; Address; Animal Model; Animals; Apoptosis; Biochemical; Biological Assay; Biological Markers; Biological Monitoring; Biological Process; Breast Carcinoma; Catalysis; Cathepsin L; Cathepsins B; Cell Death; Cells; Chemicals; Cleaved cell; Clinical; Clinical Pathology; Condition; Contrast Media; Coupled; Cultured Cells; Cytosol; Detection; Development; Diagnostic; Drug Kinetics; Endopeptidases; Enzymes; Evolution; Foundations; Goals; Human; Human Mammary Carcinoma; Hydrogen; In Situ Nick-End Labeling; In Vitro; Incubated; Infusion procedures; Invasive; Kinetics; Lesion; MCF7 cell; Magnetic Resonance Imaging; Malignant Neoplasms; Mammary Neoplasms; Methods; Monitor; Neoplasm Metastasis; Normal tissue morphology; Pathology; Peptide Hydrolases; Peptides; Protein Overexpression; Proteins; Rate; Relative (related person); Relaxation; Research; Saline; Sampling; Scanning; Signal Transduction; Solutions; Specificity; Staining method; Stains; TNFSF10 gene; Technology; Therapeutic; Therapeutic Effect; Tissues; Toxic effect; Urokinase; Water; base; caspase-3; design; extracellular; fluorescence imaging; functional group; improved; in vivo; magnetic field; malignant breast neoplasm; molecular imaging; mouse model; neoplastic cell; preclinical study; response; size; subcutaneous; success; tool; tumor

DESCRIPTION (provided by applicant): Proteases are important biomarkers for many pathologies and biological processes and are often targets of chemotherapeutics. Magnetic Resonance Imaging (MRI) is an outstanding diagnostic tool for identifying suspicious lesions, but suffers from many false-positive results due to a lack of specificity for pathological tissues versus normal tissues. The development of MRI contrast agents that can detect protease biomarkers of metastatic tumors or the response to anti-tumor therapies would greatly improve the specificity of MRI for detecting and evaluating cancer. We have developed a fundamentally new type of protease-responsive MRI contrast agent that is detected through PARAmagnetic Chemical Exchange Saturation Transfer (PARACEST). This mechanism allows each PARACEST contrast agent to be selectively detected, so that multiple PARACEST contrast agents may be simultaneously detected during a single MRI scan session, such as a protease-unresponsive PARACEST contrast agent that can serve as a `control' to account for the pharmacokinetics of the agents. Furthermore, we are the first research team to demonstrate the detection of PARACEST contrast agents within in vivo animal models. We will combine our accomplishments with PARACEST contrast agents to detect multiple enzyme-responsive PARACEST contrast agents within in vivo animal models. More specifically, we will design and apply PARACEST contrast agents to detect caspase-3 during TRAIL-induced tumor cell apoptosis in an in vivo mouse model of MCF-7c3 human mammary carcinoma. We will also design and apply PARACEST contrast agents to simultaneously detect cathepsin B and urokinase Plasminogen Activator in an in vivo mouse model of MCF-7 and MBA-MB-231 mammary carcinoma. The successful achievement of our specific aims will result in new platform technology that can be immediately used during many pre-clinical studies, and that can be used as a foundation for further studies to gain clinical approval for this new type of diagnostic molecular imaging agent to detect protease biomarkers in cancer and other pathological tissues. Narrative: A fundamentally new type of protease-responsive MRI contrast agent has been developed that is detected through PARAmagnetic Chemical Exchange Saturation Transfer (PARACEST). Furthermore, PARACEST contrast agents can be detected within in vivo animal models. This research application will combine these accomplishments with PARACEST contrast agents to detect multiple enzyme-responsive PARACEST contrast agents within in vivo animal models.

描述（由申请人提供）：蛋白酶是许多病理和生物过程的重要生物标志物，通常是化学治疗剂的靶标。磁共振成像（MRI）是用于识别可疑病变的出色诊断工具，但由于缺乏对病理组织而不是正常组织的特异性，遭受了许多假阳性结果。可以检测转移性肿瘤的蛋白酶生物标志物或对抗肿瘤疗法的反应的MRI对比剂的开发将大大提高MRI检测和评估癌症的特异性。我们已经开发了一种从根本上开发的蛋白酶响应性MRI对比剂，该对比剂是通过顺磁化学交换饱和转移（Paracest）检测到的。这种机制允许选择性检测到每种最有用的对比剂，因此可以在单个MRI扫描过程中同时检测到多种最有支位的对比剂，例如蛋白酶无反应的有针对性的对比剂，可以用作“控制”以说明特工的药代动力学。此外，我们是第一个证明体内动物模型中最有着对比对比剂的检测的研究团队。我们将将我们的成就与最有用的对比剂结合在一起，以检测体内动物模型中多种酶响应的最高对比剂。更具体地说，我们将在MCF-7C3人类乳腺癌的体内小鼠模型中设计和应用最有用的对比剂来检测caspase-3。我们还将在MCF-7和MBA-MB-231乳腺癌的体内小鼠模型中设计和应用最有用的对比剂，同时检测组织蛋白酶B和尿激酶纤溶酶原激活剂。我们的特定目标的成功实现将导致新的平台技术，可以在许多临床前研究中立即使用，并可以用作进一步研究的基础，以获得这种新型诊断分子成像剂的临床认可，以检测癌症和其他病理组织中的蛋白酶生物标志物。叙述：已经开发了一种新型的蛋白酶响应MRI对比剂，该蛋白酶通过顺磁化学交换饱和转移（Paracest）检测到。此外，可以在体内动物模型中检测到最有用的对比剂。该研究应用程序将将这些成就与最有用的对比剂结合起来，以检测体内动物模型中的多种酶反应性的最有可能的对比剂。