F. nucleatum in periodontal disease and preterm birth

具核梭杆菌在牙周病和早产中的作用

• 批准号: 7474044
• 负责人: Yiping Han
• 金额: $ 11.83万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7474044
• 关键词: Abscess; Address; Adherence; Affinity; Amniotic Fluid; Animal Model; Antibodies; Applications Grants; Award; Bacteria; Bacterial Adhesins; Basic Science; Binding; Biological Assay; Blood; Cell Communication; Cell-Matrix Junction; Characteristics; Chromatography; Clinical Research; DNA Fingerprinting; Dental; Dental Plaque; Dentistry; Doctor of Philosophy; Education; Educational process of instructing; Endothelial Cells; Epithelial; Faculty; Fetal Membranes; Funding; Fusobacteria; Fusobacterium; Fusobacterium nucleatum; Future; Goals; Gram-Negative Anaerobic Bacteria; Health; Hematogenous; Heterogeneity; Human; Immune response; In Vitro; Independent Scientist Award; Infection; Inflammatory; Injection of therapeutic agent; Institutes; Kinetics; Laboratories; Left; Location; Low Birth Weight Infant; Maternal Health; Mediating; Modeling; Mus; NIH Program Announcements; Oral; Oral cavity; Oral health; Organism; Other Body Part; Patient currently pregnant; Periodontal Diseases; Pilot Projects; Placenta; Polymerase Chain Reaction; Precipitation; Pregnant Women; Premature Birth; Premature Labor; Proliferating; Reproductive Biology; Research; Research Support; Risk Factors; Role; Sample Size; Sampling; Source; Structure; Testing; Tissues; Toll-like receptors; Translational Research; Universities; Uterus; Vagina; Virulence; Woman; career; craniofacial; crosslink; cytokine; frontier; in vivo; interest; mutant; novel; oral tissue; programs; receptor; stillbirth; Abscess; Address; Adherence; Affinity; Amniotic Fluid; Animal Model; Antibodies; Applications Grants; Award; Bacteria; Bacterial Adhesins; Basic Science; Binding; Biological Assay; Blood; Cell Communication; Cell-Matrix Junction; Characteristics; Chromatography; Clinical Research; DNA Fingerprinting; Dental; Dental Plaque; Dentistry; Doctor of Philosophy; Education; Educational process of instructing; Endothelial Cells; Epithelial; Faculty; Fetal Membranes; Funding; Fusobacteria; Fusobacterium; Fusobacterium nucleatum; Future; Goals; Gram-Negative Anaerobic Bacteria; Health; Hematogenous; Heterogeneity; Human; Immune response; In Vitro; Independent Scientist Award; Infection; Inflammatory; Injection of therapeutic agent; Institutes; Kinetics; Laboratories; Left; Location; Low Birth Weight Infant; Maternal Health; Mediating; Modeling; Mus; NIH Program Announcements; Oral; Oral cavity; Oral health; Organism; Other Body Part; Patient currently pregnant; Periodontal Diseases; Pilot Projects; Placenta; Polymerase Chain Reaction; Precipitation; Pregnant Women; Premature Birth; Premature Labor; Proliferating; Reproductive Biology; Research; Research Support; Risk Factors; Role; Sample Size; Sampling; Source; Structure; Testing; Tissues; Toll-like receptors; Translational Research; Universities; Uterus; Vagina; Virulence; Woman; career; craniofacial; crosslink; cytokine; frontier; in vivo; interest; mutant; novel; oral tissue; programs; receptor; stillbirth

DESCRIPTION (provided by applicant): Dr Yiping Han is a PhD microbiologist. Dr Han's research evolves around understanding the virulence mechanism of F. nucleatum. Since joining the faculty at Case Western Reserve University, her research interest has expanded from basic science to include more clinically oriented studies. Dr Han and her colleagues recently developed a pregnant murine model and showed a causal relationship between F. nucleatum and preterm birth. Dr Han is using F. nucleatum as a model organism to investigate the correlation between oral health and preterm birth. She has research support from NIDCR, with an R03 award (DE 14447) to investigate the source of F. nucleatum infection in amniotic fluids in women in preterm labor, and an R01 award (DE 14924) to further characterize the FadA adhesin from this organism. Support from a K02 award will allow Dr Han to forego further grant applications for the near future and to avoid projected increase in teaching and administrative responsibilities. This will permit Dr Han to devote over 80% of her effort on her research program and her plans for re-education in dentistry and reproductive biology. Long term career goals are to become a well-rounded oral microbiologist competent in both basic science and translational research. The specific aims of this application are: (i) to characterize the FadA adhesin from F. nucleatum for its role in tissue cell attachment and invasion; (ii) to identify the host receptor for FadA and to characterize its role in F. nudeatum-hosl cell interactions; and (iii) to determine if women with amniotic fluid infections of F. nucleatum harbor the same species in the vaginal track, blood, or the oral cavity.

描述（由申请人提供）：Yiping Han博士是博士学位微生物学家。 Han博士的研究围绕理解F. nucleatum的毒力机理的发展而发展。自从加入Case Western Reserve University的教职员工以来，她的研究兴趣已从基础科学扩展到包括更多临床导向的研究。 Han博士和她的同事们最近开发了一种怀孕的鼠模型，并显示了F. nutleatum和早产之间的因果关系。 Han博士将F. nutleatum用作模型生物体来研究口腔健康与早产之间的相关性。她获得了NIDCR的研究支持，并获得了R03奖（DE 14447），以调查早产妇女羊水中F. nucleatum感染的来源，并获得了R01奖（DE 14924），以进一步描述了该生物体中FADA粘合剂的特征。 K02奖的支持将使Han博士放弃在不久的将来进一步授予申请，并避免预计教学和行政责任的增加。这将使Han博士能够将超过80％的努力用于研究计划，并在牙科和生殖生物学中重新教育计划。长期职业目标是成为一名全面的口头微生物学家，在基础科学和翻译研究中都有能力。该应用的具体目的是：（i）表征FADA粘附素在组织细胞附着和侵袭中的作用； （ii）识别FADA的宿主受体并表征其在F. nudeatum-Hosl细胞相互作用中的作用； （iii）确定f.核的羊水感染的妇女是在阴道，血液还是口腔中含有相同物种的妇女。