Cellular and Molecular Mechanisms of Cytokinesis Mediated by the TIO Fused Kinase

TIO 融合激酶介导的细胞分裂的细胞和分子机制

• 批准号: BB/E001017/1
• 负责人: David Twell
• 金额: $ 41.64万
• 依托单位: University of Leicester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE001017%2F1
• 关键词: Cellular; Molecular; Mechanisms; Cytokinesis; Mediated

The division of cells in plants and animals is a vitally important process as it provides the building blocks for complex growth and development. During cell division the genetic material of the mother cell is divided into twin daughter nuclei that are split into separate daughter cells by a process known as cytokinesis. Plant and animal cells use different modes of cytokinesis. In animals this involves a pinching in process ('outside-in' mode), whereas in plants it involves building a new internal partition ('inside-out' mode). Because of the evolution of specific ways of 'splitting' plant cells and the importance of this process for plant growth, crop and food production, we are trying to understand the details of how this process is regulated in plants. To this end we have used genetic screens to identify genes that are required for cell division during pollen development in the important model plant species Arabidopsis thaliana (thale cress). This led to the isolation of two-in-one mutants that produce pollen grains with two nuclei in a common cytoplasm as a result of failure to build internal dividing walls. We have cloned the TWO-IN-ONE gene that is mutated in the two-in-one mutants and discovered that it is closely related to the FUSED gene that is present in humans and fruit flies. However unlike its animal counterparts that are not involved in cytokinesis, we have shown that TWO-IN-ONE is required for cytokinesis throughout the plant life cycle in vegetative (green) and reproductive parts of the plant and so is essential for plant growth, development and survival. In this work our objective is to understand how the TWO-IN-ONE protein is important in the cell division process using molecular, genetic and cell biology approaches. We will study the effect of making mutant forms of the TWO-IN-ONE protein to see how efficiently they function compared with the normal TWO-IN-ONE protein. We will use fluorescent proteins to monitor in living cells how the structures involved in building the new dividing wall are organised and how mutated TWO-IN-ONE proteins influences this process. We will also study other proteins that can bind with the TWO-IN-ONE protein that may help the TWO-IN-ONE protein to carry out its tasks. The outcome of this investigation will help us to explain at the cellular and molecular levels how TIO carries out its role in the plant cell division process. Moreover this work may also help us to explain how a conserved protein of ancient origin has evolved unique roles in the cell biology and life cycles of plants and animals.

动植物中细胞的分裂是一个至关重要的过程，因为它为复杂生长和发育提供了基础。在细胞分裂期间，母细胞的遗传物质被分为双子核，该核通过称为细胞因子的过程分为单独的子细胞。动植物细胞使用不同模式的细胞因子。在动物中，这涉及在过程中捏合（“外部”模式），而在植物中，它涉及建立新的内部分区（“内部”模式）。由于“分裂”植物细胞的特定方式的演变以及该过程对植物生长，作物和粮食生产的重要性，因此我们试图了解该过程如何调节植物中的细节。为此，我们使用了遗传筛选来鉴定重要模型植物植物拟南芥（Thale Cress）中花粉发育过程中细胞分裂所需的基因。这导致分离出两合一突变体，这些突变体由于未能建造内部分裂壁而在公共细胞质中产生花粉颗粒。我们已经克隆了在两合一突变体中突变的两合一基因，并发现它与人类和果蝇中存在的熔融基因密切相关。但是，与其无关胞质分裂的动物对应物不同，我们已经表明，在整个植物生命周期中，植物生命（绿色）和植物生殖部位中的细胞因子需要二合一，因此对于植物的生长，发育和生存至关重要。在这项工作中，我们的目标是了解使用分子，遗传和细胞生物学方法在细胞分裂过程中二合一蛋白如何重要。我们将研究制作两合一蛋白的突变形式的效果，以查看它们与正常二合一蛋白相比它们的有效效果。我们将使用荧光蛋白来监测活细胞中的荧光蛋白如何组织新的分隔壁的结构以及突变的两合一合一蛋白如何影响这一过程。我们还将研究其他可以与两合一蛋白结合的蛋白质，这些蛋白质可能有助于两合一的蛋白质执行其任务。这项研究的结果将有助于我们在细胞和分子水平上解释TIO如何在植物细胞分裂过程中发挥作用。此外，这项工作还可以帮助我们解释古代来源的保守蛋白质如何在植物和动物的细胞生物学和生命周期中演变出独特的作用。

项目成果

The tobacco MAP215/Dis1-family protein TMBP200 is required for the functional organization of microtubule arrays during male germline establishment.

• DOI: 10.1093/jxb/erp367
• 发表时间: 2010-02
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Oh SA;Pal MD;Park SK;Johnson JA;Twell D
• 通讯作者: Twell D

Overexpression of TWO-IN-ONE Domains Inhibits Cytokinesis in Arabidopsis

二合一结构域的过度表达抑制拟南芥中的细胞分裂

• DOI: 10.1007/s12374-022-09353-6
• 发表时间: 2022
• 期刊: Journal of Plant Biology
• 影响因子: 2.9
• 作者: Oh S