CRYSTALLOGRAPHIC DESCRIPTION OF THE PHOTOINTERMEDIATES OF BACTERIORHODOPSIN

细菌视紫红质光中间体的晶体学描述

• 批准号: 7370358
• 负责人: JANOS K LANYI
• 金额: $ 0.49万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370358
• 关键词: CRYSTALLOGRAPHIC; DESCRIPTION; PHOTOINTERMEDIATES; BACTERIORHODOPSIN; CRYSTALLOGRAPHIC; DESCRIPTION; PHOTOINTERMEDIATES; BACTERIORHODOPSIN

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacteriorhodopsin crystals we have grown in cubic lipid phase diffract to nearly 1.4 ¿¿ and promise to yield structural information on the reaction cycle intermediates, K, L, M, N and O, of this light-driven proton pump. By illumination of wild-type and mutant crystals under various conditions, a few M sub-states could be produced with 100% occupancy, but most intermediates accumulate to 40-60% only. Refinement of the second conformation in the models is difficult, and confidence in the conclusions depends on high-resolution data from numerous duplicate experiments. Data sets we collected to 1.43 ¿¿resolution have been encouraging, and produced preliminary information about the twisted retinal chromophore in the K state and its changes in an early M state. We propose to continue these experiments with the best synchrotron beam available, and to describe, for the first time for any ion pump, the transport mechanism in detailed molecular terms.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是针对该中心的，这不是调查人员的机构。细菌近红紫红色的晶体我们已经在立方脂质相衍射中生长至几乎1.4€»，并承诺对这种轻驱动质子泵的反应循环中间体，K，L，M，N和O产生结构信息。通过在各种条件下对野生型和突变体晶体的照明，可以以100％的占用率产生一些M子状态，但大多数中间体仅积累至40-60％。模型中第二个考虑的改进很困难，对结论的信心取决于来自众多重复实验的高分辨率数据。我们收集到1.43的数据集令人鼓舞，并产生了有关K状态中扭曲的视网膜发色团及其在早期M状态的变化的初步信息。我们建议使用可用的最佳同步束继续进行这些实验，并首次以详细的分子项来描述任何离子泵的传输机理。