TOMOGRAPHY OF ZIGZAG, RETIFORM AND HONEYCOMB CRISTAE IN HEART

心脏锯齿形、网状结构和蜂窝状嵴断层扫描

• 批准号: 7357293
• 负责人: YURU DENG
• 金额: $ 2.25万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357293
• 关键词: TOMOGRAPHY; ZIGZAG; RETIFORM; HONEYCOMB; CRISTAE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cubic membranes are symmetric, periodic structures that occur in a wide variety of living systems. There are several types of crystal structure proposed for the observed membrane arrays in a variety biological systems. In previous work (Deng, et al. Protozoology), a template matching method was developed to indirectly identify the crystal type using electron microscopy. The system studied was mitochondria of starved amoeba. Subsequently, we used the same material to determine the crystal type from electron tomographic reconstruction, in comparison to a computer-generated model volume of a perfect crystal of the appropriate type. This validated the template-matching method. WhileDr. Deng was a postdoc at the RVBC, we made reconstructions of mitochondria representing examples of two different crystal types, the double-diamond and the gyroid. Extensive use of computer visualization and modeling methods enabled us to not only determine the crystal type (double-diamond any gyroid) but also to understand how the cubic membranes are integrated into the architecture of the mitochondrion, giving insights into their function. We found that the spaces in the cubic area were intracristal space, that was connected to the space between the inner and outer mitochondrial membranes by tubular connecting regions as seen in the other types of mitochondria we have studied. The matrix material was narrowly confined between the membranes in the cubic phase, thus reversing the usual situation in which most of the volume inside the mitochondria is matrix material. This work is continuing, with heart mitochondria of dog and canary.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。立方膜是对称的周期性结构，存在于各种生命系统中。对于各种生物系统中观察到的膜阵列，提出了几种类型的晶体结构。在之前的工作（Deng 等人，Protozoology）中，开发了一种模板匹配方法，利用电子显微镜间接识别晶体类型。研究的系统是饥饿的阿米巴原虫的线粒体。 随后，我们使用相同的材​​料通过电子断层扫描重建来确定晶体类型，并与计算机生成的适当类型的完美晶体的模型体积进行比较。这验证了模板匹配方法。 而博士。邓是 RVBC 的博士后，我们对线粒体进行了重建，代表了两种不同晶体类型的例子：双金刚石和螺旋体。计算机可视化和建模方法的广泛使用使我们不仅能够确定晶体类型（双金刚石任意陀螺仪），而且能够了解立方体膜如何整合到线粒体的结构中，从而深入了解它们的功能。我们发现立方体区域中的空间是晶体内空间，它通过管状连接区域与线粒体内膜和外膜之间的空间连接，正如我们研究的其他类型的线粒体中所见。基质材料被狭窄地限制在立方相的膜之间，从而扭转了线粒体内大部分体积是基质材料的通常情况。 这项工作仍在继续，涉及狗和金丝雀的心脏线粒体。