TOMOGRAPHY OF ZIGZAG, RETIFORM AND HONEYCOMB CRISTAE IN HEART

心脏锯齿形、网状结构和蜂窝状嵴断层扫描

• 批准号: 7357293
• 负责人: YURU DENG
• 金额: $ 2.25万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357293
• 关键词: TOMOGRAPHY; ZIGZAG; RETIFORM; HONEYCOMB; CRISTAE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cubic membranes are symmetric, periodic structures that occur in a wide variety of living systems. There are several types of crystal structure proposed for the observed membrane arrays in a variety biological systems. In previous work (Deng, et al. Protozoology), a template matching method was developed to indirectly identify the crystal type using electron microscopy. The system studied was mitochondria of starved amoeba. Subsequently, we used the same material to determine the crystal type from electron tomographic reconstruction, in comparison to a computer-generated model volume of a perfect crystal of the appropriate type. This validated the template-matching method. WhileDr. Deng was a postdoc at the RVBC, we made reconstructions of mitochondria representing examples of two different crystal types, the double-diamond and the gyroid. Extensive use of computer visualization and modeling methods enabled us to not only determine the crystal type (double-diamond any gyroid) but also to understand how the cubic membranes are integrated into the architecture of the mitochondrion, giving insights into their function. We found that the spaces in the cubic area were intracristal space, that was connected to the space between the inner and outer mitochondrial membranes by tubular connecting regions as seen in the other types of mitochondria we have studied. The matrix material was narrowly confined between the membranes in the cubic phase, thus reversing the usual situation in which most of the volume inside the mitochondria is matrix material. This work is continuing, with heart mitochondria of dog and canary.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。立方膜是在多种生活系统中发生的对称，周期性结构。在各种生物系统中，建议观察到的膜阵列提出了几种类型的晶体结构。在先前的工作（Deng等人的原生动学）中，开发了一种模板匹配方法，以间接使用电子显微镜识别晶体类型。研究的系统是饥饿的变形虫的线粒体。 随后，与适当类型的完美晶体的计算机生成的模型体积相比，我们使用了相同的材料来确定电子断层造影重建的晶体类型。这验证了模板匹配方法。 Whiledr。 Deng是RVBC的博士后，我们对线粒体进行了重建，代表了两种不同的晶体类型的例子，即双钻石和辅助物。广泛使用计算机可视化和建模方法使我们不仅可以确定晶体类型（双钻甲状腺），还可以了解如何将立方膜整合到线粒体的体系结构中，从而深入了解其功能。我们发现，立方区域的空间是室内空间，它通过管状连接区域连接到内部和外部线粒体膜之间的空间，如我们所研究的其他类型的线粒体所示。基质材料在立方相的膜之间狭窄地限制在膜之间，从而逆转了通常的情况，即线粒体内部大部分体积是基质材料。 这项工作仍在继续，狗和金丝雀的心脏线粒体。