The role of eIF4G in translation initiation and cell cycle progression

eIF4G 在翻译起始和细胞周期进展中的作用

• 批准号: BB/D007593/1
• 负责人: Simon Morley
• 金额: $ 59.45万
• 依托单位: University of Sussex
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD007593%2F1
• 关键词: role; eIF4G; translation; initiation; cell

Critical information stored in the gene sequences of the genetic material (DNA) has to be decoded by the cell to produce a wide variety of essential proteins, as and when they are required. The sequence of each gene specifies the sequence of a given protein, with many proteins themselves in turn responsible for the synthesis of other types of structures in the cell. The general transfer of information from DNA to protein is carried out by the messenger RNA (mRNA), which is a copy of the DNA sequence and has to be decoded by a complex, highly regulated machine termed a ribosome, in a process known as translation. To work efficiently, accurately, and to allow the ribosome to function in the best interests of the cell, this machinery requires other proteins (translation initiation factors) that interact with each other, the mRNA and the ribosome in a highly regulated manner. One of these, eIF4G, acts as a scaffold protein, onto which the ribosome and several other initiation factors assemble. In mammalian cells, two slightly different genes contain the sequence for eIF4G, and the two genes also express different variants of the final protein. We wish to determine how these distinct proteins cause differences in how the ribosome and other initiation factors assemble onto and regulate the translation of different mRNAs during cell growth and division. This study will improve our understanding of the different stages of gene expression, and may also lead to the specific control of certain mRNAs that are deregulated during diseases, including cancer.

在需要时，必须将存储在遗传材料（DNA）基因序列（DNA）的基因序列（DNA）的基因序列中，以产生各种必需蛋白质。每个基因的序列指定给定蛋白的序列，许多蛋白质本身又负责细胞中其他类型的结构的合成。信息从DNA到蛋白质的一般传递是由Messenger RNA（mRNA）进行的，它是DNA序列的副本，必须由一个称为核糖体的复杂的，高度调节的机器解码，在称为翻译的过程中。为了有效，准确，允许核糖体在细胞的最大利益中发挥作用，该机械需要其他蛋白质（翻译起始因子）相互相互作用的蛋白质，以高度调节的方式，mRNA和核糖体。其中之一是EIF4G充当支架蛋白，核糖体和其他几个起始因子都组装出来。在哺乳动物细胞中，两个略有不同的基因包含EIF4G的序列，两个基因也表达了最终蛋白质的不同变体。我们希望确定这些不同的蛋白质如何导致核糖体和其他起始因子如何在细胞生长和分裂过程中调节不同mRNA的翻译。这项研究将提高我们对基因表达不同阶段的理解，也可能导致对某些在疾病（包括癌症）中受管制的mRNA的特定控制。