PURINERGIC RECEPTOR RESEARCH

嘌呤能受体研究

• 批准号: 7358044
• 负责人: PAUL A INSEL
• 金额: $ 1.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358044
• 关键词: PURINERGIC; RECEPTOR; RESEARCH

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Epithelial cells along the collecting duct regulate free water reabsorption and acid secretion through various hormones and neurotransmitters, including atrial natriuretic peptide, bradykinin, catecholamines, miner-alocorticoids, prostanoids, and vasopressin. Under certain circumstances, P2-purinoceptor agonists (ATP, UTP, ADP, and UDP) can also regulate ion transport in these cells by activating various signaling cascades. Two types of P2-purinoceptors have been described to date: P2X-purinoceptors that correspond to ligand-gated cation channels and P2Y-purinoceptors that are coupled to heterotrimeric G proteins. Both the P2X- and P2Y-purinoceptors are known to occur in several isoforms, most of which exhibit wide tissue distribution. These proteins are also diffusely distributed along various nephron segments. Previous work suggests striking differences in expression of G protein coupled receptors on polarized MDCK cells but information regarding endogenously expressed purinergic P2Y receptor subtypes is not available. We are using the Bio-rad Radiance confocal microscope and Imaris software in order to examine the localization of various purinergic receptors in MDCK D-1 cell in order to detect green fluorescent protein (GFP) labeled P2Y purinergic receptors. Specifically, we are attempting to image sagital and transverse images of confluent cell layers.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。沿着收集管道的上皮细胞通过各种激素和神经递质调节游离水的吸收和酸分泌，包括心房脂蛋白尿素肽，胸骨蛋白，儿茶酚胺，矿工 - 阿洛甲基类固醇，前类素，前类动物和加压素。在某些情况下，P2-Purinoceptor激动剂（ATP，UTP，ADP和UDP）也可以通过激活各种信号级联反应来调节这些细胞中的离子传输。迄今为止，已经描述了两种类型的P2-Purinoceptor：对应于配体门控阳离子通道和P2Y-Purinoceptor的P2X-Purinoceptor，它们与异核三聚体G蛋白偶联。已知P2X-和P2Y-Purinoceptor都发生在几种同工型中，其中大多数表现出较大的组织分布。这些蛋白质也沿各个肾单位段扩散分布。先前的工作表明，在极化MDCK细胞上G蛋白偶联受体的表达有惊人的差异，但是有关内源表达的嘌呤能P2Y受体亚型的信息。我们使用的是Bio-Rad辐射共聚焦显微镜和IMARIS软件，以检查MDCK D-1细胞中各种嘌呤能受体的定位，以检测标记为P2Y Pureinergic受体的绿色荧光蛋白（GFP）。具体而言，我们正在尝试对融合细胞层的刻板图像进行图像图像。