REDUCING THE EFFECTS OF A PHARMACOLOGICAL STRESSOR IN MONKEYS

减少药物应激源对猴子的影响

• 批准号: 7349561
• 负责人: JAMES K ROWLETT
• 金额: $ 2.8万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349561
• 关键词: REDUCING; EFFECTS; PHARMACOLOGICAL; STRESSOR; MONKEYS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The understanding of mechanisms underlying the action of anxiolytic compounds may be facilitated by the development of improved behavioral models with nonhuman primates. The alpha-2-adrenoceptor antagonist yohimbine has been shown to increase behavioral and physiological markers of mild stress in squirrel monkeys as well as subjective ratings of anxiety in humans. We explored the extent to which yohimbine-induced markers of stress in monkeys can be attenuated by a known anxiolytic (the benzodiazepine alprazolam). Adult male squirrel monkeys were administered yohimbine alone or combined with alprazolam prior to a videotaped session in an observation chamber. Following the session, salivary cortisol was determined in awake, unrestrained monkeys as described previously (Am J Primatol 60: 69-75, 2003). Videotapes were scored for the occurrence of self-directed behaviors (grooming and scratching). Yohimbine alone induced a dose-dependent increase in scratching, a behavioral marker of mild stress, but had no significant effect on grooming. Yohimbine also induced a dose-dependent increase in salivary cortisol. Pretreatment with alprazolam attenuated increases in scratching and salivary cortisol levels induced by a maximally effective dose of yohimbine.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。通过发展非人类灵长类动物的改善行为模型，可以促进对抗焦虑化合物作用的机制的理解。 α-2-肾上腺素拮抗剂Yohimbine已被证明会增加松鼠猴中轻度应激的行为和生理标记，以及人类焦虑的主观评分。我们探讨了Yohimbine诱导的猴子压力标记的程度，可以通过已知的抗焦虑（苯二氮卓类alprazolam）减弱。成年雄性松鼠猴仅在观察室进行录像带之前单独给Yohimbine或与阿普唑仑结合。会议结束后，如前所述（AM J Primatol 60：69-75，2003）确定唾液皮质醇。为出现自我指导的行为（修饰和刮擦）的录像带评分。单独的Yohimbine诱导刮擦剂量依赖性增加，这是一种轻度应激的行为标记，但对修饰没有显着影响。 Yohimbine还诱导唾液皮质醇的剂量依赖性增加。用阿普唑仑预处理会减弱由最大有效剂量Yohimimbine诱导的刮擦和唾液皮质醇水平的增加。