Nanotechnology: Detection & Analysis of Plaque Formation

纳米技术：检测

基本信息

批准号：
7234692
负责人：
Gang Bao
金额：
$ 223.59万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-05-15 至 2010-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7234692
关键词：
Address American Animal Model Antiatherogenic Antibodies Area Arterial Fatty Streak Arteries Atherosclerosis BMP4 Binding Biological Biology Biomechanics Biomedical Engineering Blood Vessels Budgets Cardiology Cardiovascular Diseases Cardiovascular system Cause of Death Cells Chymase Clinical Clinical Management Collaborations Complex Computer Simulation Condition Contrast Media Coronary artery Data Detection Development Development, Other Diagnosis Diagnostic Disease Disease Marker Doctor of Philosophy Dyes Early Diagnosis Endothelial Cells Endothelium Engineering Ensure Environment Enzymes Evaluation Event Faculty Family suidae Fluorescence Resonance Energy Transfer Fostering Foundations Functional disorder Funding Future Gadolinium Gene Expression Genes Goals Grant Growth and Development function Heart Housing Human Image Image Analysis Inflammatory Injury Intercellular adhesion molecule 1 Joints Knowledge Lesion Life Ligands Link Lipids Location Low-Density Lipoproteins Magnetic Resonance Imaging Magnetism Medical Medicine Messenger RNA Methodology Methods Modeling Molecular Molecular Analysis Molecular Probes Monitor Morbidity - disease rate Mus NAD(P)H oxidase Nanotechnology Nature Numbers Outcomes Research Oxidative Stress Paper Pathogenesis Peer Review Grants Peptides Personal Satisfaction Persons Pilot Projects Play Poly(A) Tail Postdoctoral Fellow Principal Investigator Program Development Property Protein Overexpression Proteins Publishing Quantum Dots Rate Recruitment Activity Reporter Reporting Research Research Personnel Research Project Grants Research Training Risk Role Rupture Schedule Seeds Semiconductors Sensitivity and Specificity Signal Transduction Smooth Muscle Myocytes Solutions Specificity Staging Standardization Stress Students Surface Sus scrofa System Technology Testing Tissues Training Translational Research USA Georgia United States United States National Institutes of Health Universities Update Validation Vascular Cell Adhesion Molecule-1 Vascular Diseases Western Asia Georgia Work animal tissue antibody conjugate atherogenesis base caveolin 1 college design driving force fluorophore forging gang hemodynamics human tissue improved in vivo intracellular protein transport mRNA Expression medical schools member mortality multidisciplinary nanoparticle nanoprobe nanostructured novel novel strategies oxidation programs protein localization location response shear stress size skills skills training success symposium tool

项目摘要

DESCRIPTION (provided by applicant): This PEN application aims to establish a highly collaborative and multidisciplinary Program of Excellence in Nanotechnology by integrating the bioengineering expertise of Georgia Tech (Atlanta, GA) and the cardiology strengths of Emory University School of Medicine (Atlanta, GA). The broad and long-term goal of this PEN is to develop nanotechnology and biomolecular engineering tools and methodologies for the detection and molecular analysis of atherosclerotic plaque formation, which has a compelling medical need. Cardiovascular disease remains the leading cause of death in the United States: One out of every four Americans has cardiovascular disease, and every 30 seconds one person dies from this disease. Although significant advances have been made in the management and treatment of this disease, there is still a lack of understanding of the fundamental biology and pathophysiology of atherosclerosis, especially the molecular mechanisms responsible for plaque formation. As a result, early detection of atherosclerotic plaque is difficult, leading to a high rate of morbidity and mortality. We propose to develop advanced nanotechnologies for molecular analysis of plaque formation and sensitive detection of plaques. Specifically: (1) we will develop the molecule beacon based technology for mRNA expression and localization in living cells in response to shear stress; (2) we will develop quantum dot (Qd) based probes for studying protein interactions in cells; (3) the QD probes will be further developed for in vivo detection and analysis of plaques; (4) we will develop magnetic nanoparticle probes for in vivo plaque detection; (5) we will carry out a pilot project to develop LDL and chymase based contrast agent for in vivo plaque detection; (6) as a second pilot project we will develop activatable probes for detecting oxidative stress in living cells. Underlying this PEN is a strong track record of the senior investigators who have worked together successfully in attracting joint research grants. In addition, the Department of Biomedical Engineering, which was jointly established in 1997 by Georgia Tech and Emory University School of Medicine, has presented unique opportunity for research collaboration and skill training by bring bioengineering, nanotechnologies and discoveries into medicine and vice versa. If funded, this Program of Excellence in Nanotechnology will be housed on the Emory Campus and with a truly outstanding environment for collaborative and translational cardiovascular research and training. In additional to basic knowledge on cardiology, this PEN is expected to yield methodologies that can direct impact on clinical practice of early detection, diagnosis and treatment of atherosclerosis.

描述（由申请人提供）：该笔申请旨在通过整合佐治亚理工学院的生物工程专业知识（佐治亚州亚特兰大）和埃默里大学医学院（佐治亚州亚特兰大）的心脏病学优势，以建立高度合作和多学科的纳米技术卓越课程。这支笔的广泛而长期的目标是开发纳米技术和生物分子工程工具和方法，用于对动脉粥样硬化斑块形成的检测和分子分析，这具有迫切的医疗需求。心血管疾病仍然是美国的主要死亡原因：每四个美国人中有一个患有心血管疾病，每30秒就死于这种疾病。尽管在该疾病的管理和治疗方面已经取得了重大进展，但仍缺乏对动脉粥样硬化的基本生物学和病理生理学的了解，尤其是导致斑块形成的分子机制。结果，难以早期检测动脉粥样硬化斑块，导致高发病率和死亡率高。我们建议开发晚期纳米技术，以分子分析斑块形成和斑块敏感检测。具体而言：（1）我们将开发基于灯塔的分子信标技术，以响应剪切应力而在活细胞中定位；（2）我们将开发基于量子点（QD）的探针，以研究细胞中的蛋白质相互作用；（3）QD探针将进一步开发用于体内检测和斑块分析；（4）我们将开发用于体内斑块检测的磁性纳米颗粒探针；（5）我们将执行一个试点项目，以开发基于LDL和基于Chymase的对比剂，以用于体内斑块检测；（6）作为第二个试点项目，我们将开发可激活的探针，以检测活细胞中的氧化应激。这支笔的基础是高级调查员成功地吸引联合研究补助金的高级调查员的良好记录。此外，佐治亚理工学院和埃默里大学医学院于1997年共同建立的生物医学工程系，通过将生物工程，纳米技术和发现带入医学以及反之亦然，为研究合作和技能培训提供了独特的机会。如果获得资助，该纳米技术卓越课程将安置在埃默里校园中，并为协作和转化的心血管研究和培训提供真正杰出的环境。除了心脏病学基础知识的其他知识外，预计该笔将产生对早期检测，诊断和治疗动脉粥样硬化的临床实践影响的方法。