Let your metabolites shine: new NMR experiments and hardware for enhanced studies of metabolites

让您的代谢物大放异彩：新的 NMR 实验和硬件可增强代谢物的研究

• 批准号: 2898065
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2898065
• 关键词: Let; your; metabolites; shine; new

"Nuclear magnetic resonance spectroscopy (NMR) is increasingly applied in studies of metabolites because it is arguably one of the most powerful methods for obtaining structural and dynamic information in complex mixtures. Comprehensively monitoring the patterns of metabolites can be useful to both the analysis of underlying metabolite networks (e.g. the responses to the changing environment or the presence of stress, pest or disease) and as metabolite fingerprinting approaches (e.g., to confirm a food product's authenticity or to demonstrate lack of potentially toxic adulteration [1]). One exciting possibility to further improve the value of NMR-based metabolomics - which is still lacking sensitivity compared to conventional mass spectrometry approaches - is to take advantage of photo-chemically induced dynamic nuclear polarisation (photo-CIDNP [2]), where observed NMR signal intensities of aromatic compounds in the presence of a photosensitiser are modulated and often significantly increased upon illumination. We recently proposed and patented the new way of illuminating NMR samples in situ using LEDs (NMRtorch approach [3,4]), leading to photo-CIDNP enhancements exceeding those obtained using lasers, while allowing for potentially much higher sample throughput, and even automation. The aim of the proposed project is to explore the use of photo-CIDNP for studies of aromatic metabolites, initially for the purpose of fingerprinting and authentication of plant-based liquid food products, such as grape juice or wine, and then extending it to other metabolomics systems such as biological fluids, for example for identifying and monitoring metabolite biomarkers associated with health and disease. "

“核磁共振光谱（NMR）越来越多地应用于代谢产物的研究中，因为它可以说是在复杂混合物中获得结构和动态信息的最有力的方法之一。全面监测代谢物的模式可用于对触发反应的反应和pest的疾病（例如，pester）的分析（例如，均与pesters的疾病分析）（例如方法（例如，确认食品的真实性或证明缺乏潜在的有毒掺假[1]）。在存在光感剂存在下的化合物受到调节，并在照明时通常会显着增加。我们最近提出并申请了使用LED（NMRTORCH方法[3,4]）在原位照明NMR样品的新方法，从而导致超过使用激光器获得的光周期增强功能，同时允许可能更高的样品吞吐量，甚至自动化。拟议项目的目的是探索使用Photo-CIDNP进行芳族代谢产物的研究，最初是为了指纹识别和认证植物性液体食品，例如葡萄汁或葡萄酒，然后将其延伸到其他代谢组学系统（例如生物学体液），例如识别和监测与代理生物疾病的生物学生物和疾病。 “