BIOPHILE-80C Storage Unit with Individual Vial Retriever

BIOPHILE-80C 存储单元，带独立样品瓶回收器

• 批准号: 6732444
• 负责人: JOSHUA LABAER
• 金额: $ 40.21万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6732444
• 关键词: biomedical automation; biomedical equipment purchase; cell bank /registry; clone cells; robotics

DESCRIPTION (provided by applicant): Biological research is shifting paradigms from molecular reductionist approaches to include larger-scale investigations of the entire proteome or genome. High-throughput (HT) biology began in earnest with the Human Genome Project; increasingly HT tools are being exploited for protein research. Given the importance of proteins in disease etiology and treatment, a major challenge facing biology is the elucidation of the physiological role of every protein. Using HT approaches, functional proteomics will address this challenge by expressing proteins at a scale of thousands at a time, and by subsequently assaying their functional properties, such as subcellular location, interacting partners, biochemical activity and regulated modification. A prerequisite for all these studies is a comprehensive collection of full-length expression-ready (FLEX) clones. Such a collection is being built using recombinational cloning, which allows rapid transfer into any vector, in frame and without mutation. These FLEXGene clones provide researchers with a powerful resource that expresses proteins in all experimental formats and at any chosen scale. As a complement to the FLEX repository, a collection is being assembled of clones encoding small inhibitory RNAs (siRNAs), which specifically inhibit the expression of their cognate gene. Together with FLEXGene, these collections allow users to up- or down-regulate specific proteins to study their function in biology and disease. Furthermore, assaying sets of hundreds of clones enables investigators to conduct large-scale screens to identify key proteins or pathways. The overall objective of this project is to build a robust repository that makes these clones readily available to researchers. However, the number of collected clones (already >15,000) exceeds the ability to distribute, track and retrieve them manually. User-requested sets of 200 clones can be dispersed over 100 different plates. Without automation, the fulfillment of dozens of clone requests is untenable on a just-in-time basis. This application requests funds to obtain an automated storage unit. The BIOPHILE storage unit with individual vial retriever (IVR) uniquely combines the reliability of robotically controlled retrieval with the required clone stability of -80 x C. Bar-coded samples are robotically retrieved without opening a freezer door, thus maintaining a stable temperature. While manual distribution accommodates fewer than 100 clones per day, the BIOPHILE has a capacity of 1,200 clones per day.

描述（由申请人提供）：生物学研究正在将范式从分子还原论方法转变为包括对整个蛋白质组或基因组的更大规模研究。高通量（HT）生物学真正始于人类基因组计划；越来越多的 HT 工具被用于蛋白质研究。鉴于蛋白质在疾病病因学和治疗中的重要性，生物学面临的一个主要挑战是阐明每种蛋白质的生理作用。使用 HT 方法，功能蛋白质组学将通过一次以数千个规模表达蛋白质，并随后分析其功能特性（例如亚细胞位置、相互作用伙伴、生化活性和调控修饰）来应对这一挑战。所有这些研究的先决条件是全面收集全长表达就绪 (FLEX) 克隆。这样的集合是通过重组克隆建立的，它可以快速转移到任何载体中，框内且无突变。这些 FLEXGene 克隆为研究人员提供了强大的资源，可以以所有实验形式和任何选定的规模表达蛋白质。 作为 FLEX 存储库的补充，正在组装编码小抑制性 RNA (siRNA) 的克隆集合，这些克隆特异性抑制其同源基因的表达。与 FLEXGene 一起，这些集合允许用户上调或下调特定蛋白质，以研究其在生物学和疾病中的功能。此外，分析数百个克隆组使研究人员能够进行大规模筛选以识别关键蛋白质或途径。该项目的总体目标是建立一个强大的存储库，使研究人员可以轻松使用这些克隆。 然而，收集到的克隆数量（已超过 15,000 个）超出了手动分发、跟踪和检索它们的能力。用户请求的 200 个克隆组可以分散在 100 个不同的平板上。如果没有自动化，就无法及时满足数十个克隆请求。该应用程序需要资金来获得自动存储单元。带有独立小瓶回收器 (IVR) 的 BIOPHILE 存储单元独特地将机器人控制回收的可靠性与所需的 -80 x C 克隆稳定性结合起来。无需打开冷冻室门即可自动回收条形码样品，从而保持稳定的温度。虽然手动分配每天可容纳的克隆数量少于 100 个，但 BIOPHILE 每天可容纳 1,200 个克隆。