Laser Scanning Confocal Microscope for Genetic Research

用于基因研究的激光扫描共焦显微镜

• 批准号: 7212037
• 负责人: BARRY S GANETZKY
• 金额: $ 34.17万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7212037
• 关键词: 3-Dimensional; Address; Arts; Biological; Biological Process; Biology; Blindness; Cell division; Congenital Abnormality; Development; Disease; Embryo; Faculty; Funding; Funding Applicant; Gene Expression Regulation; Genetic; Genetic Research; Health; Human; Image; Investigation; Laboratories; Laboratory Organism; Lasers; Leadership; Maintenance; Malignant Neoplasms; Memory; Mental Retardation; Microscope; Molecular; Mus; Nerve Degeneration; Optics; Organ; Proteins; Range; Research; Research Training; Resolution; Scanning; Scientist; Signal Transduction; Specimen; Thick; Training; Training Programs; United States National Institutes of Health; Universities; Wisconsin; Yeasts; college; deafness; in vivo; insight; instrument; programs; reconstruction; relating to nervous system; tool

DESCRIPTION (provided by applicant): Funds are requested to purchase a Zeiss LSM 510 laser-scanning confocal microscope for integration into the Genetics Training Program (GTP) Imaging Facility. The University of Wisconsin GTP encompasses more than 75 faculty trainers in 10 departments and 3 colleges. It is recognized as one of the premier programs worldwide for research and training in Genetics. To maintain and advance our leadership in this field and to continue to train the most promising scientists, it is essential to have access to the state-of-the-art instruments and facilities on which our research depends. A confocal microscope has become one of the most essential research tools in modern biology, regardless of the particular experimental organism or particular problem under investigation. However, we currently lack consistent, affordable access to a confocal microscope. To fill this need, we are applying here for funds to purchase a confocal microscope. Eight primary users who will manage and subsidize maintenance of the microscope are submitting this application on behalf of the entire GTP. With reliable access to a confocal microscope, GTP scientists will be able to (i) simultaneously observe the cellular and sub-cellular localization of three different molecules (ii) follow the in vivo expression and localization dynamics of multiple proteins; (iii) visualize thin optical sections of thick biological specimens; and (iv) obtain high-resolution 3-D reconstructions of their specimens. The addition of a confocal microscope to our imaging facility will have a major impact on the research programs of each of the primary users. These programs range from studies of yeast to mice, and address fundamental questions in biology from the regulation of gene expression to the molecular mechanisms of cell division, from embryonic and organ development to neural signaling and memory formation. Relevance: The ongoing studies of the primary users are yielding important insights into the biological processes underlying human disorders including cancer, birth defects, mental retardation, deafness, blindness, and neurodegeneration, among others. We also will attract new users from within the GTP, so an accessible confocal will broaden and strengthen the research programs of laboratories beyond those of the initial primary users. All of the GTP faculty are engaged in research of significant relevance to human health; most have current funding through NIH.

描述（由申请人提供）：需要资金购买 Zeiss LSM 510 激光扫描共焦显微镜，以便集成到遗传学培训计划 (GTP) 成像设施中。威斯康星大学 GTP 拥有 10 个系和 3 个学院的超过 75 名教员培训师。它被公认为全球遗传学研究和培训的首要项目之一。为了保持和提升我们在这一领域的领导地位并继续培训最有前途的科学家，必须获得我们研究所依赖的最先进的仪器和设施。无论所研究的特定实验生物体或特定问题如何，共聚焦显微镜已成为现代生物学中最重要的研究工具之一。然而，我们目前缺乏一致的、负担得起的共聚焦显微镜。为了满足这一需求，我们在这里申请资金购买共焦显微镜。将管理和补贴显微镜维护的八名主要用户代表整个 GTP 提交此申请。通过可靠地使用共焦显微镜，GTP 科学家将能够 (i) 同时观察三种不同分子的细胞和亚细胞定位 (ii) 跟踪多种蛋白质的体内表达和定位动态； (iii) 使厚生物样本的薄光学切片可视化； (iv) 获得其标本的高分辨率 3D 重建。在我们的成像设施中添加共焦显微镜将对每个主要用户的研究项目产生重大影响。这些项目涵盖从酵母到小鼠的研究，解决生物学中的基本问题，从基因表达的调节到细胞分裂的分子机制，从胚胎和器官发育到神经信号传导和记忆形成。相关性：对主要用户正在进行的研究正在对人类疾病的生物学过程产生重要的见解，包括癌症、出生缺陷、智力迟钝、耳聋、失明和神经退行性疾病等。我们还将吸引 GTP 内部的新用户，因此易于使用的共聚焦将扩大和加强实验室的研究计划，超越最初的主要用户。 GTP 的所有教员都从事与人类健康密切相关的研究；大多数目前都通过 NIH 获得资助。