Novel microfluidics for rapid high-performance biosensing detection

用于快速高性能生物传感检测的新型微流体

• 批准号: 2889885
• 金额: --
• 依托单位: University of Bath
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2889885
• 关键词: Novel; microfluidics; rapid; performance; biosensing

Non-communicable diseases are the largest cause of death worldwide, with a key one being cardiovascular diseases. Millions of people live with chronic cardiac conditions requiring regular monitoring of disease progression, also millions of people across the globe report acute cardiovascular incidents, such as pulmonary embolism or myocardial infarction. In both chronic and acute cardiac situations, it is highly desirable to have access to decentralized high-performance bioanalytical equipment offering a short time from analysis to results. Decentralized, high-performance quantitative equipment is not yet available, requiring transformative developments in biosensing, sample preparation, and miniaturization. This PhD project aims to develop an automated microfluidic-based immuno-biosensing platform for the detection of multiple biomarkers of cardiovascular diseases at a single point in time. Biomarkers like D-dimer, brain natriuretic peptide, and C-reactive protein have been shown to be good predictors of early detection of cardiovascular diseases. The ability to measure these biomarkers simultaneously at the point-of-need without access to expensive equipment would be transformative for the management and prevention of cardiovascular diseases.In particular, we aim to develop a sensing platform that is flexible and cost-effective. From a production perspective, we aim to use scalable and easily accessible materials such as polymer-based sensing platforms. Furthermore, the project aims to explore the possibility of using a high-tech and rapid printing technique for the mass production of sensors and their fabrication on various surfaces. This would not only help us lower production costs but also make these sensing platforms more widely available and accessible to patients and healthcare professionals worldwide. Additionally, by utilizing a high-tech printing technique, we can ensure the sensors are consistently accurate and reliable. In advance, we will focus on the utilization of nanoparticles to enhance the selectivity and sensitivity of the sensor. Additionally, the platform will be designed to be user-friendly, allowing individuals to easily perform the test themselves without the need for specialized training or expertise. We will deliver an advanced microfluidic detection platform built around the 'microfluidic siphon' concept pioneered by the PhD supervisor, enabling automation of high-performance immunoassays with multiplexed samples at high-throughput and low sample consumption. We will present a new advanced biosensing approach that can measure a variety of cardiac biomarkers quantitatively from a small sample of blood or human serum. We will validate the sensor using electrochemical measurements such as Electrical Impedance Spectroscopy (EIS), voltammetry, and optical immune-biosensing. Development of the microfluidic designs will be assisted with Multiphysics and computational fluid dynamics simulation software like COMSOL and Ansys Fluent.dictors of early detection of cardiovascular diseases. The ability to measure simultaneously these biomarkers in the point-of-need without access to expensive equipment would be transformative for the management and prevention of cardiovascular diseases.

非传染性疾病是全球死亡的最大原因，关键是心血管疾病。数以百万计的人患有慢性心脏病，需要定期监测疾病进展，全球数百万的人报告了急性心血管事件，例如肺栓塞或心肌梗塞。在慢性和急性心脏状况中，非常需要获得分散的高性能生物分析设备，从分析到结果，提供短时间的时间。分散的，高性能的定量设备尚不可用，需要在生物传感，样品制备和小型化中进行变革性发展。该博士学位项目旨在开发一个基于微流体的免疫生物传感平台，用于在一个时间点检测多种心血管疾病的生物标志物。诸如D-二聚体，脑发脂肽和C反应蛋白等生物标志物已被证明是早期发现心血管疾病的良好预测指标。在不使用昂贵设备的情况下同时测量这些生物标志物的能力将对管理和预防心血管疾病进行变革。尤其是，我们旨在建立一个灵活且具有成本效益的传感平台。从生产的角度来看，我们的目标是使用可扩展且易于访问的材料，例如基于聚合物的传感平台。此外，该项目旨在探索使用高科技和快速打印技术来大规模生产传感器及其在各种表面上的制造的可能性。这不仅可以帮助我们降低生产成本，而且还可以使这些传感平台在全球的患者和医疗保健专业人员中更广泛地获得和访问。此外，通过使用高科技打印技术，我们可以确保传感器始终准确且可靠。事先，我们将专注于利用纳米颗粒来增强传感器的选择性和灵敏度。此外，该平台将设计为用户友好，使个人可以轻松地自己执行测试，而无需进行专业培训或专业知识。我们将提供一个先进的微流体检测平台，围绕由PHD主管启用的“微流体Siphon”概念建立，从而使高性能免疫测定自动化具有高性异性样品，并在高通量和样本消耗下自动化。我们将提出一种新的高级生物传感方法，该方法可以从一小部分血液或人血清中定量测量各种心脏生物标志物。我们将使用电化学测量值（例如电阻抗光谱（EIS），伏安法和光学免疫传感）来验证传感器。微流体设计的开发将通过多物理和计算流体动力学仿真软件（如Comsol和Ansys Fluent）的帮助。同时在不使用昂贵设备的情况下同时测量这些生物标志物的能力将对管理和预防心血管疾病进行变革。