HYBRID HYDROGELS SELF-ASSEMBLED FROM GRAFT COPOLYMERS

由接枝共聚物自组装的杂化水凝胶

• 批准号: 7173402
• 负责人: JINDRICH H. KOPECEK
• 金额: $ 28.71万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7173402
• 关键词: Abbreviations; Accounting; Acetic Acid; Acetic Acids; Affinity; Alanine; Arginine; Asparagine; Aspartic Acid; Atomic Force Microscopy; Biocompatible Materials; Biological; Biosensor; Characteristics; Charge; Circular Dichroism; Class; Coiled-Coil Domain; Compatible; Condition; Cyclohexanecarboxylic Acids; Cysteine; Dextrans; Differential Scanning Calorimetry; Drosophila genus; Drug Delivery Systems; Encapsulated; Environment; Equilibrium; Ethylene Glycols; Feedback; Flavin Mononucleotide; Fluorescence Resonance Energy Transfer; Glutamic Acid; Glutamine; Glycine; Goals; Guanidinium Chloride; Hand; Helix (Snails); Histidine; Hybrids; Hydrogels; Hydrophobic Interactions; Immunoglobulins; In Situ; Ionic Strengths; Ions; Isoleucine; Kinesin; Label; Length; Leucine; Liquid substance; Lysine; MALDI-TOF Mass Spectrometry; Mediating; Modeling; Molecular Sieve Chromatography; Molecular Structure; Molecular Weight; Muscle; N-(2-hydroxypropyl)methacrylamide; N-(4-carboxycyclohexylmethyl)maleimide N-hydroxysuccinimide ester; Nature; Numbers; Ornithine; Peptides; Permeability; Pharmaceutical Preparations; Phase Transition; Phenylalanine; Polyacrylamide Gel Electrophoresis; Polymerase Chain Reaction; Polymers; Process; Propane; Property; Proteins; Range; Rate; Recording of previous events; Research; Research Personnel; Sampling; Scanning Electron Microscopy; Serine; Side; Sodium Dodecyl Sulfate; Solutions; Solvents; Structure; Surface; Swelling; System; Temperature; Tertiary Protein Structure; Theoretical model; Tryptophan; Tyrosine; Valine; Vertebral column; aqueous; azobis(isobutyronitrile); base; copolymer; crosslink; cyclohexanecarboxylic acid; density; design; dextran; dimer; ear helix; epidoxorubicin glucuronide; ethylene glycol; hydroxypropyl methacrylate; macromolecule; methacrylamide; nanometer; programs; research study; self assembly; stimulus sensitivity; theories; tool

DESCRIPTION (provided by applicant): The goal of the project is to design new macromolecular structures, whose assembly into hybrid hydrogels will be modulated by association of coiled-coil peptide domains. N-(2-hydroxypropyl)rnethacrylamide (HPMA) copolymers containing coiled-coil domains as side-chains (grafts) will be synthesized by copolymerization of HPMA with macromonomers containing coiled-coils. The design of coiled-coil grafts imposes an antiparallel heterodimer formation in the self-assembly process. We hypothesize that the antiparallel orientation of heterodimers will contribute to the homogeneity of the self-assembled hybrid hydrogels due to the unique interchain dimer formation and decreased steric hindrance of the polymer backbone on the "in-register" alignment of heterodimers. Materials with tailor-made structures at the nanometer range will result from the proposed research. A theoretical model will be developed to assist in a rational design of the hybrid hydrogels. Experimental results will be analyzed using the model to provide feedback for the optimization of graft copolymer structure and of the self-assembly into hybrid hydrogels. The self-assembly of HPMA graft copolymers in the presence of encapsulated molecules will be evaluated in an attempt to develop new in-situ forming hybrid hydrogels for the delivery of proteins. The delivery system is suitable for a wide variety of biological molecules since the self-assembly occurs in aqueous media. The new hydrogels may find wide-ranging biomedical applications in drug delivery, biosensors, affinity separations, and nanoreactors. In addition, the possibility to join non-compatible materials, such as hydrophobic muscle mimics from liquid crystalline polymers and hydrogels, may open a new field of research.

描述（由申请人提供）： 该项目的目的是设计新的大分子结构，其组装成混合水凝胶将通过盘绕螺旋肽域的关联来调节。 N-（2-羟丙基）rnethacrylamide（HPMA）共聚物含有卷曲螺旋域（作为侧链）（移植物），将通过HPMA与含有串联圈的大型经济体共聚的HPMA合成。盘绕螺旋移植的设计在自组装过程中施加了反平行的异二聚体形成。我们假设异二聚体的反平行方向将有助于自组装混合水凝胶的同质性，这是由于独特的互链二聚体形成并减少了聚合物主链在“杂二聚体内注册”对齐时的空间障碍。在纳米范围内具有裁缝结构的材料将源于拟议的研究。 将开发一种理论模型来协助混合水凝胶的合理设计。将使用模型分析实验结果，以提供优化移植共聚物结构以及自组装到杂化水凝胶中的反馈。 将在封装分子存在下进行HPMA移植共聚物的自组装，以尝试开发新的原位形成杂种水凝胶以递送蛋白质。由于自组装发生在水性介质中，因此递送系统适用于多种生物分子。新的水凝胶可能在药物输送，生物传感器，亲和力分离和纳米反应器中发现广泛的生物医学应用。此外，加入非兼容材料的可能性，例如液晶晶体聚合物和水凝胶的疏水性肌肉模仿，可能会开设一个新的研究领域。