A New Nano Based Real-Time Mycotoxin Detector

新型纳米实时霉菌毒素检测仪

基本信息

批准号：
7214528
负责人：
NICHOLAS J SMILANICH
金额：
$ 22.66万
依托单位：
SENSOR DEVELOPMENT CORPORATION
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-24 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7214528
关键词：
Acute Affect Aflatoxin B1 Aflatoxins Agriculture Anabolism Animals Aspergillus Aspergillus flavus Attention Carcinogens Cereals Cessation of life Characteristics Chemistry Condition Country Crows Data Dependence Detection Development Devices Disease Disease Outbreaks Domestic Animals Ear Economics Environment Evaluation Exhibits Farming environment Feeds Food Food Chain Food Safety Food Supply Fumonisins Fusarium Gases Goals Government Growth Hand Health Health Hazards High Pressure Liquid Chromatography Housing Human Immune Immunity Ingestion International Agency for Research on Cancer Laboratories Laboratory Animals Lead Left Life Life Cycle Stages Livestock Maize Marketing Measures Meat Medical Device Mercury Metabolic Metabolic Pathway Methodology Microbiology Milk Molds Molecular Weight Monitor Mycotoxins Numbers Nuts Osmium Peanuts - dietary Penicillium Persons Phase Plants Poison Poisoning Practice Management Preparation Process Production Purpose Quick Test for Liver Function Range Research Sampling Semiconductors Standards of Weights and Measures Techniques Technology Temperature Testing Time Toxin USA Georgia United States Food and Drug Administration Universities Western Asia Georgia Wheat Work Zea mays base catalyst combinatorial chemistry cost deoxynivalenol detector disease characteristic feeding follow-up fungus humulene innovation metal oxide microbial nano nutrition planetary Atmosphere plant fungi programs prototype response sensor size stannic oxide toe corn vapor volatile organic compound

项目摘要

DESCRIPTION (provided by applicant): We believe that unique volatile signatures are associated with fungal outbreaks associated with production of aflatoxin. The long-term goal of the project is to demonstrate the feasibility of our innovative nano-crystalline tin oxide sensor detecting these unique Microbial Volatile Organic Compounds (MVOCs) at very low levels and differentiating between low levels of these unique organic vapors and other Volatile Organic Compounds and MVOC's. The resulting detector would be set inside a grain storage container to protect food safety. The first aim in this research is to identify a catalyst and operating condition, which allow these unique volatiles to be selectively detected in crop enclosures containing both the unique volatiles and potential identified interferants. Combinatorial chemistry methodology will be used to establish the conditions for unique volatile selectivity. Secondly, our aim is to provide baseline information, under biologically relevant conditions to substantiate the use of particular volatiles as markers of aflatoxin contamination. This will be accomplished by carrying out research on host plant-fungus interactions involved in the aflatoxin contamination process and doing volatile analysis on different fungal strains. The conductance response of the sensor is proportional to the concentration of these gases; that is, the higher the concentration of the gas, the greater the response. At the conclusion of this project, we expect to have demonstrated proof of principle for a sensor capable of selectively determining concentrations of unique mold related volatiles as low as 1 ppb in the presence of other typical crop related volatiles. Aflatoxins are toxic metabolites produced by certain molds in/on foods and feeds. The substance is so toxic that it is one of 19 contaminants-along with mercury and DDT-for which the U.S. Food and Drug Administration imposes strict tolerance levels in food, with only trace amounts allowed. Aflatoxins have been associated with various diseases, such as aflatoxicosis, in livestock, domestic animals and humans throughout the world. Aflatoxins have received greater attention than any other mycotoxins because of their demonstrated potent carcinogenic effect in susceptible laboratory animals and their acute toxicological effects in humans. The greatest threat of health hazards to humans come from long term exposures of tainted food products, either from spoilage or from consuming milk or meat from animals that have been fed contaminated feed. The USDA noted that countries that lack the means to regulate and monitor the presence of aflatoxin leave their inhabitants open to unknown poisonings that can continue over long periods of time. Throughout the world, approximately 4.5 billion persons are chronically exposed to largely uncontrolled amounts of the toxin where the toxic affect of aflatoxin on immunity and nutrition combine to negatively affect health factors. Mycotoxins are carcinogenic compounds that are products of the mold Aspergillus. They affect up to 30% of the worlds food supply and the resulting conclusion is that approximately 4.5 billion persons worldwide are chronically exposed to largely uncontrolled amounts of the toxin. No animal species including humans are immune to aflatoxin poisoning with the greatest threat of health hazards to humans coming from long-term exposure of tainted food products, either from spoilage or from consuming milk or meat from animals that have been fed contaminated feed.

描述（由申请人提供）：我们认为，独特的挥发性特征与黄曲霉毒素产生相关的真菌爆发有关。该项目的长期目标是展示我们创新的纳米晶体氧化锡传感器检测极低含量的这些独特的微生物挥发性有机化合物 (MVOC) 并区分低含量的这些独特的有机蒸气和其他挥发性有机化合物的可行性化合物和 MVOC。由此产生的探测器将安装在粮食储存容器内，以保护食品安全。这项研究的首要目标是确定催化剂和操作条件，从而可以在含有独特挥发物和潜在已识别干扰物的农作物围栏中选择性地检测到这些独特的挥发物。将使用组合化学方法来建立独特的挥发性选择性的条件。其次，我们的目标是在生物学相关条件下提供基线信息，以证实使用特定挥发物作为黄曲霉毒素污染的标记物。这将通过对黄曲霉毒素污染过程中涉及的宿主植物-真菌相互作用进行研究并对不同真菌菌株进行挥发性分析来实现。传感器的电导响应与这些气体的浓度成正比；也就是说，气体浓度越高，响应越大。在该项目结束时，我们期望能够证明传感器的原理证明，该传感器能够在存在其他典型作物相关挥发物的情况下选择性地确定低至 1 ppb 的独特霉菌相关挥发物的浓度。黄曲霉毒素是食品和饲料中/表面的某些霉菌产生的有毒代谢物。这种物质的毒性如此之大，以至于它是美国食品和药物管理局在食品中规定严格耐受水平的 19 种污染物之一（与汞和滴滴涕一起），仅允许微量存在。黄曲霉毒素与全世界牲畜、家畜和人类的多种疾病有关，例如黄曲霉毒素中毒。黄曲霉毒素比任何其他霉菌毒素受到更多关注，因为它们对易感实验动物具有强效致癌作用，对人类具有急性毒理学作用。对人类健康危害的最大威胁来自于长期接触受污染的食品，无论是腐败变质还是食用了被污染饲料喂养的动物的奶或肉。美国农业部指出，缺乏监管和监测黄曲霉毒素存在手段的国家，其居民容易遭受未知的中毒，这种中毒可能会持续很长时间。在全世界，大约有 45 亿人长期接触大量不受控制的毒素，其中黄曲霉毒素对免疫和营养的毒性作用结合起来对健康因素产生负面影响。霉菌毒素是致癌化合物，是霉菌曲霉菌的产物。它们影响了世界上高达 30% 的粮食供应，由此得出的结论是，全世界大约有 45 亿人长期接触数量基本上不受控制的毒素。包括人类在内的任何动物物种都不能免受黄曲霉毒素中毒的影响，对人类健康危害的最大威胁来自于长期接触受污染的食品，无论是腐败变质还是食用来自受污染饲料的动物的奶或肉。