A New Nano Based Real-Time Mycotoxin Detector

新型纳米实时霉菌毒素检测仪

基本信息

批准号：
7214528
负责人：
NICHOLAS J SMILANICH
金额：
$ 22.66万
依托单位：
SENSOR DEVELOPMENT CORPORATION
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-24 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7214528
关键词：
Acute Affect Aflatoxin B1 Aflatoxins Agriculture Anabolism Animals Aspergillus Aspergillus flavus Attention Carcinogens Cereals Cessation of life Characteristics Chemistry Condition Country Crows Data Dependence Detection Development Devices Disease Disease Outbreaks Domestic Animals Ear Economics Environment Evaluation Exhibits Farming environment Feeds Food Food Chain Food Safety Food Supply Fumonisins Fusarium Gases Goals Government Growth Hand Health Health Hazards High Pressure Liquid Chromatography Housing Human Immune Immunity Ingestion International Agency for Research on Cancer Laboratories Laboratory Animals Lead Left Life Life Cycle Stages Livestock Maize Marketing Measures Meat Medical Device Mercury Metabolic Metabolic Pathway Methodology Microbiology Milk Molds Molecular Weight Monitor Mycotoxins Numbers Nuts Osmium Peanuts - dietary Penicillium Persons Phase Plants Poison Poisoning Practice Management Preparation Process Production Purpose Quick Test for Liver Function Range Research Sampling Semiconductors Standards of Weights and Measures Techniques Technology Temperature Testing Time Toxin USA Georgia United States Food and Drug Administration Universities Western Asia Georgia Wheat Work Zea mays base catalyst combinatorial chemistry cost deoxynivalenol detector disease characteristic feeding follow-up fungus humulene innovation metal oxide microbial nano nutrition planetary Atmosphere plant fungi programs prototype response sensor size stannic oxide toe corn vapor volatile organic compound

项目摘要

DESCRIPTION (provided by applicant): We believe that unique volatile signatures are associated with fungal outbreaks associated with production of aflatoxin. The long-term goal of the project is to demonstrate the feasibility of our innovative nano-crystalline tin oxide sensor detecting these unique Microbial Volatile Organic Compounds (MVOCs) at very low levels and differentiating between low levels of these unique organic vapors and other Volatile Organic Compounds and MVOC's. The resulting detector would be set inside a grain storage container to protect food safety. The first aim in this research is to identify a catalyst and operating condition, which allow these unique volatiles to be selectively detected in crop enclosures containing both the unique volatiles and potential identified interferants. Combinatorial chemistry methodology will be used to establish the conditions for unique volatile selectivity. Secondly, our aim is to provide baseline information, under biologically relevant conditions to substantiate the use of particular volatiles as markers of aflatoxin contamination. This will be accomplished by carrying out research on host plant-fungus interactions involved in the aflatoxin contamination process and doing volatile analysis on different fungal strains. The conductance response of the sensor is proportional to the concentration of these gases; that is, the higher the concentration of the gas, the greater the response. At the conclusion of this project, we expect to have demonstrated proof of principle for a sensor capable of selectively determining concentrations of unique mold related volatiles as low as 1 ppb in the presence of other typical crop related volatiles. Aflatoxins are toxic metabolites produced by certain molds in/on foods and feeds. The substance is so toxic that it is one of 19 contaminants-along with mercury and DDT-for which the U.S. Food and Drug Administration imposes strict tolerance levels in food, with only trace amounts allowed. Aflatoxins have been associated with various diseases, such as aflatoxicosis, in livestock, domestic animals and humans throughout the world. Aflatoxins have received greater attention than any other mycotoxins because of their demonstrated potent carcinogenic effect in susceptible laboratory animals and their acute toxicological effects in humans. The greatest threat of health hazards to humans come from long term exposures of tainted food products, either from spoilage or from consuming milk or meat from animals that have been fed contaminated feed. The USDA noted that countries that lack the means to regulate and monitor the presence of aflatoxin leave their inhabitants open to unknown poisonings that can continue over long periods of time. Throughout the world, approximately 4.5 billion persons are chronically exposed to largely uncontrolled amounts of the toxin where the toxic affect of aflatoxin on immunity and nutrition combine to negatively affect health factors. Mycotoxins are carcinogenic compounds that are products of the mold Aspergillus. They affect up to 30% of the worlds food supply and the resulting conclusion is that approximately 4.5 billion persons worldwide are chronically exposed to largely uncontrolled amounts of the toxin. No animal species including humans are immune to aflatoxin poisoning with the greatest threat of health hazards to humans coming from long-term exposure of tainted food products, either from spoilage or from consuming milk or meat from animals that have been fed contaminated feed.

描述（由申请人提供）：我们认为，独特的挥发性特征与黄曲霉毒素的生产相关的真菌暴发有关。该项目的长期目标是证明我们创新的纳米结晶锡氧化物传感器的可行性，以非常低的水平检测这些独特的微生物挥发性有机化合物（MVOC），并区分低水平的这些独特的有机蒸气和其他挥发性有机化合物和其他挥发性有机化合物和MVOC。最终的检测器将设置在谷物存储容器中，以保护食品安全。这项研究的第一个目的是确定催化剂和工作条件，这使得在包含独特挥发物和潜在鉴定的干扰物的作物外壳中选择性检测到这些独特的挥发物。组合化学方法将用于建立独特挥发性选择性的条件。其次，我们的目的是在生物学上相关的条件下提供基线信息，以证实使用特定挥发物作为黄曲霉毒素污染的标志。这将通过对黄曲霉毒素污染过程中涉及的宿主植物 - 膜相互作用进行研究来实现，并对不同的真菌菌株进行挥发性分析。传感器的电导响应与这些气体的浓度成正比。也就是说，气体的浓度越高，响应越大。在该项目的结论中，我们希望在存在其他典型的农作物相关挥发物的情况下，能够选择性地确定能够确定独特霉菌相关挥发物浓度低至1 ppb的传感器的原理证明。黄曲霉毒素是食物和饲料中某些霉菌产生的有毒代谢产物。该物质是如此的毒性，以至于美国食品和药物管理局在汞和DDT-For中的污染物和DDT-FOR中是一种污染物之一，仅允许痕量的粮食耐受性。黄曲霉毒素与世界各地的各种疾病，例如黄曲霉病，牲畜，家畜和人类有关。黄曲霉毒素比任何其他霉菌毒素都受到更大的关注，因为它们在易感实验动物中表现出了有效的致癌作用及其对人类的急性毒理学作用。对人类的健康危害的最大威胁来自对污染食品的长期暴露，无论是变质，还是来自已喂养污染饲料的动物的牛奶或肉类。美国农业部指出，缺乏监管和监测黄曲霉毒素的能力的国家使他们的居民敞开了，持续了很长一段时间，这些中毒可能会持续下去。在全世界，长期暴露于大约45亿人的毒素中，在很大程度上暴露于未受控制的毒素中，在这种毒素中，黄曲霉毒素对免疫和营养的毒性影响相结合，对健康因素产生负面影响。霉菌毒素是致癌化合物，是霉菌的产物。它们影响了世界上30％的粮食供应，得出的结论是，全世界约有45亿人长期暴露于毒素的不受控制。没有包括人类在内的动物物种不受黄曲霉毒素中毒的影响，对人类的健康危害最大的威胁来自污染食品的长期暴露，无论是由于变质，牛奶或牛奶或肉类的食用或肉类的肉类而被喂食受污染的饲料。