a/LCI Optical Biopsy System

a/LCI 光学活检系统

• 批准号: 7395213
• 负责人: WILLIAM J BROWN
• 金额: $ 13.39万
• 依托单位: SOUTHEAST TECHINVENTURES, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-29 至 2008-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7395213
• 关键词: Adopted; Animal Model; Basal Cell; Biomedical Engineering; Biopsy; Cancerous; Cellular Morphology; Characteristics; Clinical; Clinical Research; Compatible; Computer Systems Development; Computer software; Consultations; Detection; Diagnostic; Diagnostic Neoplasm Staging; Diagnostic Procedure; Endoscopes; Endoscopy; Epithelial; Epithelium; Esophageal; Esophageal Tissue; Evaluation; Excision; Facility Construction Funding Category; Fiber; Fiber Optics; Fixatives; Goals; Health; Human; In Situ; Interferometry; Intraepithelial Neoplasia; Laboratories; Lesion; Letters; Light; Malignant Neoplasms; Measurement; Medical Surveillance; Methods; Modeling; Monitor; Morphology; Names; Neoplastic Cell Transformation; Nuclear; Optical Biopsy; Optics; Premalignant; Principal Investigator; Procedures; Property; Publishing; Purpose; Range; Rate; Rattus; Research; Risk; Sampling; Scheme; Screening procedure; Staging; Staining method; Stains; Sterility; Surface; System; Techniques; Testing; Time; Tissue Sample; Tissues; Universities; Waxes; Work; animal tissue; base; carcinogenesis; clinical application; computerized data processing; data acquisition; density; design; improved; in vivo; instrument; light scattering; novel; prototype; research study; size; success; tissue phantom; tool

DESCRIPTION (provided by applicant): The objective of this research is to create a commercial prototype of a new optical biopsy system based on angle-resolved Low Coherence Interferometry (a/LIC). A/LCI is a novel optical technique that uses interferometric detection of elastically scattered light to probe the nuclear morphology of esophageal epithelium. The ability of a/LCI to obtain quantitative measurements of cellular characteristics, such as nuclear size and optical density, without the need for either exogenous stains or fixatives, makes it an excellent candidate to become a screening tool for in vivo detection of pre-cancerous tissue states such as intraepithelial neoplasia. As its name implies, a/LCI detects the angular distribution of scattered light using a low-coherence interferometer. The technique enables selective detection of the optical field scattered from a small region within probed tissue by exploiting the coherence properties of broadband light. The ability to probe sub-surface cellular morphology is especially important for detecting pre- cancerous changes in epithelial tissues, where changes in the basal cell layer, approximately 100 5m beneath the surface are the most important diagnostic of tissue health. The long-range goal of this research is to create a new a/LCI-based biomedical diagnostic product for the purpose of endoscopically identifying and monitoring neoplastic transformations in humans. The research plan, proposed here, will create a prototype which can be applied in clinical studies to evaluate the feasibility of using a/LCI for tissue screening and surveillance by achieving the following milestones: (1) constructing a new a/LCI system prototype capable of rapid data acquisition through a coherent fiber bundle, (2) advancing the prototype design by implementing a novel fiber probe geometry suitable for application during endoscopic procedures, and (3) testing the functionality of the new prototype by examining ex vivo tissue sample from a rat model for esophageal cancer.The objective of this research is to develop a new instrument for detecting early stage cancer in human epithelial tissues based on a novel optical spectroscopic technique, The proposed a/LCI instrument will serve as a guide to biopsy for clinicians, enabling evaluation of tissue health in situ, prior to tissue removal. This technique has the potential to greatly improve upon the current method of random biopsy for screening for and surveillance of early stage epithelial cancers.

描述（由申请人提供）：本研究的目的是创建基于角度分辨低相干干涉仪（A/LIC）的新光活检系统的商业原型。 A/LCI是一种新型的光学技术，它使用弹性散射光的干涉测量检测来探测食道上皮的核形态。 A/LCI获得细胞特征（例如核大小和光密度）的定量测​​量的能力，而无需外源性污渍或固定剂，使其成为成为体内检测前癌性组织（例如上皮内肿瘤）体内筛查工具的绝佳候选者。顾名思义，A/LCI使用低碳的干涉仪检测散射光的角度分布。该技术通过利用宽带光的相干性能，可以选择性检测从探测组织中的小区域散射的光场。探测地下细胞形态的能力对于检测上皮组织的癌前变化尤为重要，其中基底细胞层的变化，表面以下约100 5m是最重要的诊断组织健康的诊断。 这项研究的远程目标是创建一种新的基于A/LCI的生物医学诊断产品，以便内镜下识别和监测人类的肿瘤转化。此处提出的研究计划将创建一个原型，可以在临床研究中应用，以评估通过实现以下里程碑来实现组织筛选和监视的可行性：（1）通过在连贯的纤维束中实现适当的探针（2）实现probe的应用程序（2）构建新的A/LCI系统原型（2）构建可用于快速数据获取的新A/LCI系统原型（2）2）内窥镜检查以及（3）通过检查大鼠模型的过体组织样品来测试新原型的功能，以进行食管癌。这项研究的目的是开发一种新的工具，用于检测基于人类上皮组织的早期癌症，基于人类上皮组织的早期癌症，基于新型光学光谱仪器的新型光学仪器，以评估A/LCI的指南，以促进A/LCI的指南，以促进卫生指南，作为一名卫生指南，作为一名卫生仪式，作为一名卫生仪式，作为一名卫生指南，以此为一定原位，在切除组织之前。该技术有可能大大改善当前的随机活检方法，以筛查和监视早期上皮癌。