High Trapping Efficiency Ion Trap Mass Spectrometer

高捕获效率离子阱质谱仪

• 批准号: 6935108
• 负责人: Houle Wang
• 金额: $ 10.21万
• 依托单位: MICHROM BIORESOURCES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2005-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935108
• 关键词: analytical method; bioengineering /biomedical engineering; biomedical equipment development; electrospray ionization mass spectrometry; energy source; mass spectrometry; proteomics

DESCRIPTION (provided by applicant): The objective of this proposal is to demonstrate the feasibility of a high trapping efficiency ion trap. This device can be used on ion trap based mass spectrometers including ion trap mass spectrometers (ITMS) and hybrid quadrupole ion trap time-of-flight mass spectrometers (QitTOF). The proposed ion trap will use a specially constructed split ring connected to a pair of matched RF voltage sources, to provide a quadrupole field that can be switched between 2D (linear trap) and 3D (Paul trap) modes electronically. Using the linear trap mode for trapping (injecting) ions, a projected 10 to 20 fold improvement in trapping efficiency can be achieved versus a 3D ion trap. After the ions are trapped and cooled inside the trap, the RF electronics can be switched back to the conventional 3D ion trap mode to scan and eject the ions. The trapping efficiency improvement would thus result in a significant improvement in sensitivity. The 3D scan and eject mode of this ion trap would provide a tight output ion beam, making for a simple interface to additional components such as a TOF MS or external fragmentation device. This configuration could be applied to current and future analytical instruments that use an ion trap, as well as to other ion trap applications, allowing one to take advantage of the best of both the 2D linear trap and the 3D Paul trap. Development of the proposed device could improve the performance of several analytical tools for proteomics research. For low abundant protein samples, it could provide much better sensitivity, with lower detection limits. The impact of ion trap technology development has been seen in many areas of life science research, and this proposed work should help continue this ion trap evolution. This work will not only benefit ion trap related mass spectrometer development, but will also expand our knowledge base of ion traps in general.

描述（由申请人提供）：该提案的目的是证明高捕获效率离子阱的可行性。该设备可用于基于离子阱的质谱仪，包括离子阱质谱仪 (ITMS) 和混合四极杆离子阱飞行时间质谱仪 (QitTOF)。所提出的离子陷阱将使用一个特殊构造的开口环连接到一对匹配的射频电压源，以提供可以在 2D（线性陷阱）和 3D（保罗陷阱）模式之间以电子方式切换的四极场。使用线性陷阱模式捕获（注入）离子，与 3D 离子陷阱相比，预计捕获效率可提高 10 至 20 倍。离子在陷阱内被捕获并冷却后，射频电子设备可以切换回传统的 3D 离子陷阱模式以扫描和喷射离子。因此，捕获效率的提高将导致灵敏度的显着提高。该离子阱的 3D 扫描和喷射模式将提供紧密的输出离子束，从而为 TOF MS 或外部碎片设备等其他组件提供简单的接口。这种配置可应用于当前和未来使用离子阱的分析仪器以及其他离子阱应用，从而使人们能够充分利用 2D 线性陷阱和 3D Paul 陷阱的优点。 所提出的设备的开发可以提高蛋白质组学研究的几种分析工具的性能。对于低丰度蛋白质样品，它可以提供更好的灵敏度和更低的检测限。离子阱技术发展的影响已在生命科学研究的许多领域中体现出来，这项拟议的工作应该有助于继续离子阱的发展。这项工作不仅有利于离子阱相关质谱仪的开发，而且还将扩展我们的离子阱知识库。