EPR SPECTROMETER: VIRAL DNA PACKAGING, PROTEIN STRUCTURE

EPR 光谱仪：病毒 DNA 包装、蛋白质结构

• 批准号: 7335100
• 负责人: David D Thomas
• 金额: $ 2.5万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335100
• 关键词: DNA; Europe; aging; bioenergetics; biophysics; calcium; heart failure; membrane fusion; metal complex; metalloproteins; muscle; myosins; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A group of NIH-funded investigators plans to acquire a Bruker E680 electron paramagnetic resonance (EPR) spectrometer, to enhance their research on a wide range of biomedical problems. This instrument will offer two features that are not available in a commercial spectrometer in the entire upper Midwest: (1) a high-field superconducting magnet and the associated high-frequency (94 GHz, "W-band") operation, and (2) pulsed excitation and the associated time-domain detection. With this instrument, commercial EPR instrumentation has undergone a revolution similar to that of NMR in the 80s and 90s, with the advent of higher frequencies and time-domain technology providing dramatic improvements in sensitivity and resolution, providing deeper and more direct physical insight into molecular structure, dynamics, and interactions. This has produced an explosion of research opportunities, opening up whole new areas of investigation in site-directed spin- labeling (SDSL) and analysis of paramagnetic metal complexes. These instruments, manufactured only by Bruker in Germany, have already been installed in labs throughout Europe, but very few have been installed in the United States, and there is no such instrument in Minnesota, Wisconsin, or Iowa. Thus the requested instrument is needed if researchers in this region are to continue their high level of productivity in EPR applications, such as the following: Myosin Structural Dynamics (Thomas, Rayment, Titus, and Nesmelov), Regulation of Cardiac Calcium Transport (Thomas and Veglia), Biophysics of Muscle Aging (Thompson and Thomas), Metalloprotein Structure and Mechanism (Lipscomb and Que), SNARE-mediated Membrane Fusion (Shin), Structural Dynamics of Viral DNA Packaging (Anderson), and Bioenergetics of Heart Failure (Bache and Nesmelov)

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：一组由NIH资助的研究人员计划获得Bruker E680电子顺磁共振（EPR）光谱仪，以增强他们对广泛生物医学问题的研究。该仪器将提供两个在整个中西部上部的商业光谱仪中不可用的功能：（1）高场超导磁体和相关的高频（94 GHz，“ W-band”）操作，以及（2）脉冲激发和相关的时间域检测。借助该工具，商业EPR仪器进行了一场与80年代和90年代NMR相似的革命，随着较高频率和时间域技术的出现，提供了敏感性和分辨率的巨大改善，从而为分子结构，动力学和相互作用提供了更深入，更直接的物理洞察力。这产生了研究机会的爆炸式增长，为定向定向的自旋标记（SDSL）和顺磁金属复合物的分析打开了全新的调查领域。这些仪器仅由布鲁克在德国制造，已经安装在整个欧洲的实验室中，但在美国很少安装，并且在明尼苏达州，威斯康星州或爱荷华州没有这样的乐器。因此，如果该地区的研究人员要在EPR应用中继续其高水平的生产率，例如：肌球蛋白结构动力学（Thomas，Rayment，Titus和Nesmelov），对心脏钙转运（Thomas和Veglia）的调节，肌肉的结构动力学（Thomas and veglia），肌肉衰减（Thompson and ThomaS）的生物物理学，METALPSON和METALLOPTIN机构（MET ALLOPROTE）（METAL）（MET），网罗介导的膜融合（SHIN），病毒DNA包装的结构动力学（Anderson）以及心力衰竭的生物能学（Bache和Nesmelov）