600 MHZ NMR & PROBES: PROTEIN STRUCTURE, CARCINOGENESIS

600兆赫核磁共振

• 批准号: 7335065
• 负责人: John M Flanagan
• 金额: $ 10万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335065
• 关键词: X ray; biochemistry; brain; carcinogenesis; human; income; macromolecule; medicine; molecular biology; muscle; neoplasm /cancer; noise; pharmacology; physiology; protein structure; proteins; university; X ray; biochemistry; brain; carcinogenesis; human; income; macromolecule; medicine; molecular biology; muscle; neoplasm /cancer; noise; pharmacology; physiology; protein structure; proteins; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application is a new request for partial funding of a three channel 600 MHz NMR spectrometer, and conventional pulse gradient inverse triple resonance probe {1H, 13C, 15N} in the Pennsylvania State University College of Medicine (COM). This new instrument will compliment the recently ordered 500 MHz spectrometer for the COM campus and together provide state-of-the-art capabilities to a wide range of research groups here at the COM as well as several groups from the PSU main campus. The College of Medicine, the Departments of Biochemistry and Molecular Biology, Pharmacology, Cellular and Molecular Physiology, the Cancer Institute, and the PSU Huck Institute (Life Sciences Consortium) have 35% matching funds for these purchases. An additional institutional commitment is provide in the form of COM funds to purchase a 1H, 13C, {15N} inverse triple resonance TCI cryoprobe, 100% salary support for three years and 50% salary support thereafter for the recently hired NMR facility manager in the Department of Biochemistry and Molecular Biology and full funding of the operation of the instrument during its first year. The proposed 600 MHz NMR and ordered 500 MHz instruments will be located on the second floor of the main building (Crescent) which was deemed the best suited for these instruments based upon a site planning report from the Bruker OMNI service. In addition to the renovations need the NMR spectrometers additional renovations are planned in the surrounding modules that will allow the placement of a number of other shared instruments including several mass spectrometers, graphics workstations, and X-ray crystallographic facility. The COM has an excellent record in the administration and upkeep of shared instruments. The requested spectrometer will support the research efforts in the COM and at the PSU UP campus studying protein structure and dynamics for a range of medically relevant proteins and also several groups in the COM studying metabolic flux in human brain and muscle. The cryoprobe technology has been demonstrated to provide a significant improvement in signal-to-noise ratios for studies of biological relevant macromolecules and for metabolic flux analysis. This will allow researcher groups here and at the PSU main campus to perform technically demanding experiments such as studying the unfolded state structure of proteins, determining the structures of large proteins or protein complexes, and following metabolic flux using 13C-labeled metabolites. In addition, for many experiments the cryoprobe provides increased throughput that will accelerate the NIH supported research projects allowing and allow a larger group of researches access to this instrument.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：此申请是向宾夕法尼亚州立大学医学院（COM）在宾夕法尼亚州州立大学学院（COM）中在宾夕法尼亚州州立大学中的三个频道600 MHz NMR光谱仪部分资金的新要求。该新仪器将为COM校园提供最近订购的500 MHz光谱仪，并为COM的各种研究小组以及PSU主校园的几个小组提供最先进的功能。医学院，生物化学和分子生物学系，药理学，细胞和分子生理学，癌症研究所以及PSU Huck Institute（Life Sciences Consortium）拥有35％的购买资金。以COM资金的形式提供了另一项机构承诺，以购买1h，13c，{15n}逆三重共振TCI Cryope，三年的100％薪金支持以及此后最近雇用的NMR NMR设施经理的薪金支持50％，在该工具和完整的工具中，该部门的运营部门和分子生物学和全部融资。拟议的600 MHz NMR并订购了500 MHz仪器，将位于主建筑物的二楼（Crescent），该仪器根据Bruker Omni Service的现场计划报告，认为最适合这些乐器。除了装修外，还计划在周围的模块中进行其他翻新，这将允许放置许多其他共享工具，包括几个质谱仪，图形工作站和X射线晶体学设施。 COM在管理和保养共享工具方面具有出色的记录。所需的光谱仪将支持COM和PSU UP校园的研究工作，研究蛋白质结构和动力学的一系列医学相关蛋白质以及COM研究人脑和肌肉中代谢通量的几个组。已经证明，该冷冻探针技术可为生物学相关大分子和代谢通量分析的研究提供显着改善的信噪比。这将使研究人员在这里和PSU主校园进行技术要求进行技术要求的实验，例如研究蛋白质的展开状态结构，确定大蛋白或蛋白质复合物的结构，并使用13C标记的代谢物进行代谢通量。此外，对于许多实验，该冷冻探针提供了增加的吞吐量，该吞吐量将加速NIH支持的研究项目，并允许更多的研究访问该工具。