UW Genome Center Large-scale Sequencing Program

华盛顿大学基因组中心大规模测序计划

• 批准号: 6744072
• 负责人: MAYNARD V. OLSON
• 金额: $ 183.06万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6744072
• 关键词: artificial chromosomes; biomedical automation; biotechnology; computer assisted sequence analysis; cooperative study; genetic library; genetic mapping; genetic techniques; genome; human genetic material tag; nucleic acid sequence; restriction fragment length polymorphism; technology /technique development

DESCRIPTION: (provided by applicant): The University of Washington Genome Center (UWGC) proposes continued participation in the National Human Genome Research Institute (NHGRI) Research Network for Large-Scale Sequencing. The UWGC has participated in the network since its inception in 1998 and is on track to contribute approximately 140 Mbp of finished human sequence to the Human Genome Project (HGP). To achieve this goal, the UWGC has implemented a highly automated process for the finishing stages of large-scale sequencing. The UWGC has also put in place sufficient capacity to produce raw sequencing data to support its finishing efforts, as well as raw-data collection for other sequencing projects on a scale of tens of megabase pairs. For large projects, including much of the UWGC's contributions to the HGP, the UWGC has pioneered large-scale outsourcing of raw-data production, a model that is likely to be increasingly relevant to large-scale sequencing as raw sequencing data become a commodity that can be routinely produced. The UWGC proposes, as its next production objective, to resolve gaps and suspected large-scale misassemblies associated with genomic duplications, in the April, 2003, release of the human-reference sequence. A unique feature of the proposal is the use of new technology developed at the UWGC for comparing high-resolution, clone-based physical maps of targeted regions in multiple humans. Hence, the proposed work will improve the quality of the human-reference sequence in targeted regions, confirm that the sequence of these regions reflects the common sequence organization found in the human population, and provide information about the extent of structural polymorphism in the region. When appropriate multiple alternative sequences of structurally polymorphic regions will be determined. Other capabilities of the UWGC such as its automated finishing capacity will be made available to the NHGRI network on an as-needed basis.

描述：（由申请人提供）：华盛顿大学基因组中心（UWGC）提议继续参与大型测序的国家人类基因组研究所（NHGRI）研究网络。 自1998年成立以来，UWGC已参加网络，并有望为人类基因组项目（HGP）贡献大约140 MBP的人类序列。 为了实现这一目标，UWGC为大规模测序的精加工阶段实施了高度自动化的过程。 UWGC还具有足够的能力来生产原始测序数据以支持其完成工作，以及以数十兆巴对的规模来支持其他测序项目的原始数据集合。 对于大型项目，包括UWGC对HGP的大部分贡献，UWGC开创了RAW-DATA生产的大规模外包，该模型可能与大规模测序有关，因为原始测序数据成为一种成为一种大规模测序，因为原始测序数据成为一种成为一种，成为一种成为一种产品，成为一种原始测序数据，成为一种这种模型可以定期生产。 UWGC在2003年4月的《人参考序列》的释放中，提出了作为其下一个生产目标，以解决与基因组重复相关的大规模杂种差距。 该提案的一个独特特征是使用UWGC开发的新技术来比较多个人类的靶向区域的高分辨率，基于克隆的物理图。 因此，拟议的工作将提高目标区域中人参考序列的质量，并确认这些区域的序列反映了人口中发现的共同序列组织，并提供了有关该地区结构性多态性程度的信息。 当将确定结构多态性区域的多个替代序列时。 UWGC的其他功能（例如其自动化装饰能力）将在NHGRI网络中提供，并需要提供。