Lipid Core

脂质核心

• 批准号: 6946093
• 负责人: Lawrence L Rudel
• 金额: $ 10.29万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6946093
• 关键词: alternative medicine; analytical chemistry; biomedical facility; blood lipid; blood lipoprotein; cholesterol; cholesterol esters; dietary lipid; dietary supplements; gas chromatography mass spectrometry; high performance liquid chromatography; leukotrienes; nutrition related tag; plant extracts; prostaglandins; triglycerides; unsaturated fatty acids; vegetable oils

This core will provide basic lipid and lipoprotein analyses for each project in the Center. This core has been in existence for over 12 years and the methodology to be employed by the core is therefore highly developed. The techniques for each of the procedures to be used are described in detail. The core will provide: analysis of plasma lipid concentrations using standardized enzymatic assays; analysis of fatty acids and prostaglandins by GC/MS; analysis of plasma lipoprotein distributions for cholesterol and triglycerides by HPLC; analysis of plasma and tissue lipid compositions using enzymatic assays for major lipid classes; analysis of lipid fatty acid compositions of major lipid classes by GLC; analysis of cholesterol and cholesteryl ester concentrations by GLC; leukotriene and HETE analyses by HPLC. The technology available in this core laboratory will greatly facilitate the research in each of the projects.

该核心将为中心的每个项目提供基本的脂质和脂蛋白分析。该核心已经存在超过 12 年，因此该核心所采用的方法已经高度发展。详细描述了要使用的每个过程的技术。核心内容将提供： 使用标准化酶法分析血浆脂质浓度；通过 GC/MS 分析脂肪酸和前列腺素；通过 HPLC 分析血浆脂蛋白中胆固醇和甘油三酯的分布；使用酶法分析主要脂质类别的血浆和组织脂质成分；通过 GLC 分析主要脂质类别的脂质脂肪酸组成；通过 GLC 分析胆固醇和胆固醇酯浓度；通过 HPLC 分析白三烯和 HETE。该核心实验室现有的技术将极大地促进每个项目的研究。