MITOCHONDRIAL STRUCTURAL CHANGES IN CARDIOPROTECTION

心脏保护中的线粒体结构变化

• 批准号: 7181366
• 负责人: James N Weiss
• 金额: $ 0.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181366
• 关键词: bioimaging /biomedical imaging; biomedical resource; image processing; technology /technique development; transmission electron microscopy; bioimaging /biomedical imaging; biomedical resource; image processing; technology /technique development; transmission electron microscopy

DESCRIPTION: The National Center for Microscopy and Imaging Research (NCMIR) was established to develop computer aided advanced microscopy for acqusition of structural and functional data in the dimensional range of 1 nm3 to 100 um3. With novel specimen staining methods, imaging instruments and computational capabilities, researchers are addressing the next great biological challenges in the post-genomic age by situating proteins and macromolecular complexes in their cellular and tissue environements. Resource instruments include intermediate high voltage transmission electron microscopes (IVEMs) and high-speed large-format laser-scanning light microscopes specially modified for "meso-scale" biological microscopy. Our core technological research is directed towards developing technologies that will contribute to structural neurobiology, cell and molecular biology. With NCMIR's participation in the Biomedical Informatics Research Network (BIRN), we are scaling up our ability to image at high resolution across wide expanses of brain tissue to reveal structural and molecular in mouse models of human disease. Our collaboration, service, training and dissemination programs expand the use of these technologies to maximize their value to the biomedical community. Core biological projects include development of new staining methods for IVEM and for correlated light and IVEM analyses of specimens to examine the architecture of neuronal systems and the dynamics of subcellular processes. Core research is tightly linked to collaborative projects, in areas such as animal of neurodegenerative diseases, stroke, heart disease, cancer, infectious diseases and diabetes. The capabilites and research accomplishments anticipated in specimen preparation are augmented by continued development of instruments and image processing facilities. We emphasize the use of computer-aided methods for enhancing image contrast and reconstructing larger structural complexes using 3D tomographic and serial thick section analysis. Extraction of 3D information on a large scale will be accelerated through the development of high-throughput methods for tomographic reconstruction, analysis and visualization. We continue to provide researchers greater access to the Resource through development of web-based remote control systems for the IVEM's and interfaces for computational grids and distributed databases via high-speed networks. We will expand outreach efforts via web-based tutorials, software and microscopic data repositories linked to other biological databases.

描述：建立了国家显微镜和成像研究中心（NCMIR），以开发计算机辅助的高级显微镜，以在1 nm3至100 um3的维度范围内进行结构和功能数据。利用新型的标本染色方法，成像仪器和计算能力，研究人员通过在其细胞和组织环境中置于蛋白质和大分子复合物，来解决后基因组时代的下一个巨大生物学挑战。资源工具包括中间高压传输电子显微镜（IVEMS）和高速大型激光扫描光显微镜，该光显微镜专门修饰用于“中尺度”生物学显微镜。我们的核心技术研究致力于开发有助于结构性神经生物学，细胞和分子生物学的技术。随着NCMIR参与生物医学信息学研究网络（BIRN），我们正在扩大跨大脑组织范围内高分辨率图像的能力，以揭示小鼠的结构和分子 人类疾病的模型。我们的合作，服务，培训和传播计划扩展了这些技术的使用，以最大程度地提高其对生物医学界的价值。核心生物学项目包括开发用于IVEM和相关光的新染色方法 以及对标本的IVEM分析，以检查神经元系统的结构和亚细胞过程的动力学。核心研究与合作项目紧密相关，例如神经退行性疾病，中风，心脏病，癌症，传染病和糖尿病等领域。通过持续开发工具和图像处理设施，可以增强样品制备中预期的毛细管和研究成就。我们强调使用计算机辅助方法来增强图像对比度，并使用3D断层扫描和串行厚部分分析重建较大的结构复合物。通过开发层析成像重建，分析和可视化的高通量方法，将加速大规模提取3D信息。我们通过开发基于Web的遥控系统的IVEM和界面，通过高速网络为IVEM和分布式数据库提供了基于Web的遥控系统和界面，从而为研究人员提供了更大的访问权限。我们将通过基于Web的教程，软件和微观数据存储库来扩展外展工作，这些教程，软件和微观数据存储库与其他生物数据库相关。