TRANSIENT INFRARED PROBING OF PROTEIN FOLDNG AND CONFORMATIONAL DYNAMICS

蛋白质折叠和构象动力学的瞬态红外探测

• 批准号: 7183267
• 负责人: FENG GAI
• 金额: $ 6.73万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7183267
• 关键词: biomedical resource

DESCRIPTION (adapted from the applicant's description): The thrust of the project is to develop and employ ground-breaking methods in the use of infrared spectroscopy for the study of biological macromolecules. Developments will include infrared analogues of NMR spectroscopy for the determination of the dynamics of structure change; heterodyned 2D and 3D IR methods for study of amide I and N-H stretch modes of proteins; pump/probe methods with IR pulses, and methods to determine energy transport between remote C=O and N-H or O-H groups in peptides and proteins; methods to measure and interpret IR photon echoes and vibrational frequency correlation functions essential for understanding the response functions needed for 2D IR; instrumentation to advance T-jumps associated with IR, and of mixing kinetic methods by multiplexing infrared detection; T-jumps and mixing methods using IR probes of the intermediates; immobilization techniques to examine equilibrium fluctuations and kinetics of proteins, and other assemblies such as cells, using single-molecule fluorescence methods; methods to study ultrafast light-induced processes in biological systems probed by optical and infrared methods. There are numerous collaborative projects designed to push the technologies being developed. These involve studies on reaction centers, rhodopsin and bacteriorhodopsin, the ribosome and t-RNA, LH2 single assemblies and terahertz band-to-band transitions, pentapeptide 2D IR, calmodulin, NO-myoglobin, 310 helicies, single coiled-coils, folding and unfolding of proteins, ?-helix 2D IR, interleukin 5, ?-interferon, single ion channels, cytochrome-c trehalose, prophyrin arrays, nitrile as a probe of conformations, triplet state singles, DNA base sequence probes, and rapid-mixing folding studies. There are service projects proposed in ultrafast optical and infrared, two-dimension and single -molecule spectroscopy, in areas of DNA helicases, protein folding, serpin-serine protease interaction, laser-induced pH juimps, porphyrin electon transfer, chemosensors, helix-coil transitions, ribonucleotide reductase, light-harvesting self assembly, and NorFES single peptide study. The Resource expects to publish two newsletters each year. Besides regular seminars they will hold a synmposium on multidimensional spectroscopies and a workshop in advances in infrared laser spectroscopy . The Resource will train visiting faculty researchers, post doctorals, and graduate and undergraduate students in the applicaitons of lasers to biology.

描述（改编自申请人的描述）：该项目的主旨是开发和采用突破性的方法，利用红外光谱研究生物大分子。 发展将包括核磁共振波谱的红外类似物，用于确定结构变化的动态；用于研究蛋白质的酰胺 I 和 N-H 拉伸模式的外差 2D 和 3D IR 方法；使用红外脉冲的泵/探针方法，以及确定肽和蛋白质中远程 C=O 和 N-H 或 O-H 基团之间能量传输的方法；测量和解释红外光子回波和振动频率相关函数的方法对于理解二维红外所需的响应函数至关重要；推进与红外相关的 T 跳跃的仪器，以及通过多重红外检测混合动力学方法；使用中间体的红外探针的 T 跳跃和混合方法；使用单分子荧光方法检查蛋白质和其他组件（例如细胞）的平衡波动和动力学的固定技术；研究通过光学和红外方法探测的生物系统中超快光诱导过程的方法。 有许多合作项目旨在推动正在开发的技术。 这些涉及反应中心、视紫红质和细菌视紫红质、核糖体和 t-RNA、LH2 单一组装体和太赫兹带间转换、五肽 2D IR、钙调蛋白、NO-肌红蛋白、310 螺旋、单卷曲螺旋、折叠和蛋白质解折叠、β-螺旋 2D IR、白细胞介素 5、 β-干扰素、单离子通道、细胞色素-c 海藻糖、卟啉阵列、腈作为构象探针、三重态单峰、DNA 碱基序列探针和快速混合折叠研究。 在超快光学和红外、二维和单分子光谱、DNA 解旋酶、蛋白质折叠、丝氨酸蛋白酶-丝氨酸蛋白酶相互作用、激光诱导 pH 突变、卟啉电子转移、化学传感器、螺旋线圈等领域提出了服务项目转变、核糖核苷酸还原酶、光捕获自组装和 NorFES 单肽研究。 该资源预计每年出版两份时事通讯。 除了定期举办的研讨会外，他们还将举办多维光谱学研讨会和红外激光光谱学进展研讨会。 该资源将为访问学者、博士后以及研究生和本科生提供激光生物学应用方面的培训。