TRANSIENT INFRARED PROBING OF PROTEIN FOLDNG AND CONFORMATIONAL DYNAMICS

蛋白质折叠和构象动力学的瞬态红外探测

• 批准号: 7183267
• 负责人: FENG GAI
• 金额: $ 6.73万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7183267
• 关键词: biomedical resource

DESCRIPTION (adapted from the applicant's description): The thrust of the project is to develop and employ ground-breaking methods in the use of infrared spectroscopy for the study of biological macromolecules. Developments will include infrared analogues of NMR spectroscopy for the determination of the dynamics of structure change; heterodyned 2D and 3D IR methods for study of amide I and N-H stretch modes of proteins; pump/probe methods with IR pulses, and methods to determine energy transport between remote C=O and N-H or O-H groups in peptides and proteins; methods to measure and interpret IR photon echoes and vibrational frequency correlation functions essential for understanding the response functions needed for 2D IR; instrumentation to advance T-jumps associated with IR, and of mixing kinetic methods by multiplexing infrared detection; T-jumps and mixing methods using IR probes of the intermediates; immobilization techniques to examine equilibrium fluctuations and kinetics of proteins, and other assemblies such as cells, using single-molecule fluorescence methods; methods to study ultrafast light-induced processes in biological systems probed by optical and infrared methods. There are numerous collaborative projects designed to push the technologies being developed. These involve studies on reaction centers, rhodopsin and bacteriorhodopsin, the ribosome and t-RNA, LH2 single assemblies and terahertz band-to-band transitions, pentapeptide 2D IR, calmodulin, NO-myoglobin, 310 helicies, single coiled-coils, folding and unfolding of proteins, ?-helix 2D IR, interleukin 5, ?-interferon, single ion channels, cytochrome-c trehalose, prophyrin arrays, nitrile as a probe of conformations, triplet state singles, DNA base sequence probes, and rapid-mixing folding studies. There are service projects proposed in ultrafast optical and infrared, two-dimension and single -molecule spectroscopy, in areas of DNA helicases, protein folding, serpin-serine protease interaction, laser-induced pH juimps, porphyrin electon transfer, chemosensors, helix-coil transitions, ribonucleotide reductase, light-harvesting self assembly, and NorFES single peptide study. The Resource expects to publish two newsletters each year. Besides regular seminars they will hold a synmposium on multidimensional spectroscopies and a workshop in advances in infrared laser spectroscopy . The Resource will train visiting faculty researchers, post doctorals, and graduate and undergraduate students in the applicaitons of lasers to biology.

描述（根据申请人的描述进行了改编）：该项目的推力是开发和采用突破性方法，用于使用红外光谱法进行生物大分子研究。 发展将包括NMR光谱的红外类似物，以确定结构变化的动力学；用于研究酰胺I和N-H伸展蛋白的研究的2D和3D IR方法；带有IR脉冲的泵/探针方法，以及确定肽和蛋白质中远程C = O和N-H或O-H组之间能量传输的方法；测量和解释IR光子回声和振动频率相关功能的方法，对于了解2D IR所需的响应函数所需的方法；仪器以推进与IR相关的T突变，并通过多重红外检测来混合动力学方法；使用中间体的IR探针的T-跳跃和混合方法；使用单分子荧光方法检查蛋白质的平衡波动和动力学的固定技术；研究通过光学和红外方法探测的生物系统中超快光诱导过程的方法。 有许多协作项目旨在推动正在开发的技术。 这些涉及对反应中心，视紫红素和细菌近红淡天，核糖体和T-RNA，LH2单一组件以及Terahertz带对波段的转变，五肽2D IR，钙调蛋白，无毛糖蛋白，310螺旋素，310螺旋素，310个螺旋蛋白，单盘螺旋蛋白，柔软的蛋白质，prot蛋白，prote蛋白，prote蛋白，prot蛋白，prot蛋白，五型IR？ ？ - 未植物，单离子通道，细胞色素-C海藻糖，先知阵列，氮作为构象的探针，三重态状态单，DNA碱基序列探针和快速混合折叠研究。 在DNA解放酶，蛋白质折叠，塞旋蛋白 - 丝氨酸蛋白酶相互作用，激光诱导的pH JUIMP，卟啉转移，化学蛋白选举，化学蛋白转移，螺旋螺旋螺旋蛋白纤维酶，核糖核纤维酶，以及核糖纤维中的彼此相关，核糖核替代剂，在超快的光学和红外线，二维和单分子光谱中提出的服务项目。 学习。 资源期望每年发布两个新闻通讯。 除了常规研讨会外，他们还将在多维光谱镜和红外激光光谱方面的研讨会上持有synmposium。 该资源将培训访问的教师研究人员，邮政博士以及生物学申请的研究生和本科生。