Acquisition of Peptide Synthesizer for Core Facility

购置肽合成仪作为核心设施

• 批准号: 6876442
• 负责人: SAMUEL H. GELLMAN
• 金额: $ 12.2万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876442
• 关键词: biomedical equipment purchase; combinatorial chemistry; genetics; peptide chemical synthesis; peptide library; proteomics; biomedical equipment purchase; combinatorial chemistry; genetics; peptide chemical synthesis; peptide library; proteomics

DESCRIPTION (provided by applicant): In the current era of burgeoning proteomics and genomics efforts, the needs of campus researchers for custom peptides is expanding at a great rate. Funds are requested to purchase a state-of-the-art peptide synthesizer and a combinatorial peptide synthesizer to meet the synthetic needs of a large group of NIH funded researchers at UW-Madison. The equipment would be housed in the existing Peptide Synthesis core facility of the University of Wisconsin Biotechnology Center. A full-time PhD level Facility Manager oversees this facility. In addition, there are two full-time support staff and two student hourly employees. The facility has witnessed a remarkable increase in the campus demand for custom peptides. The number of peptides synthesized has grown steadily from 183 peptides in 1996 to over a thousand in the year 2003. Over the past 12 months the core facility has made peptides for approximately 50 campus research groups, representing 25 different departments. Current demand already exceeds the capacity of the facility and some of the existing equipment is nearing the end of its useful life. In addition to full custom synthesis carried out by core lab personnel on a cost recovery basis, the peptide core lab also offers a unique program of "do-it-yourself" peptide synthesis. Under this program, graduate students in the laboratories of the primary and secondary users are trained by the core facility personnel to operate the synthesizers and carry out their own synthesis in the core lab at reduced cost.

描述（由申请人提供）：在迅速发展的蛋白质组学和基因组学努力的时代，校园研究人员对定制肽的需求正在以极大的速度扩展。要求资金购买最先进的肽合成器和组合肽合成器，以满足UW-Madison的大量NIH资助研究人员的合成需求。该设备将安置在威斯康星大学生物技术中心的现有肽合成核心设施中。全职博士级设施经理负责监督此设施。此外，还有两名全职支持人员和两个学生小时员工。该设施见证了校园对定制肽的需求的显着增加。合成的肽数量从1996年的183种肽稳步增长到2003年的一千多个。在过去的12个月中，核心设施为大约50个校园研究小组制造了肽，代表25个不同部门。当前的需求已经超过了设施的能力，一些现有设备即将结束其使用寿命的尽头。 除了由核心实验室人员在成本恢复基础上进行的完整自定义合成外，肽核心实验室还提供了独特的“ do-youraff yousf”肽合成程序。根据该计划，核心设施人员培训了主要和二级用户实验室的研究生，以降低成本在核心实验室中操作合成器并在核心实验室中进行自己的合成。