Glutathione Peroxidase & Redox State in Atherosclerosis

谷胱甘肽过氧化物酶

基本信息

批准号：
7122935
负责人：
FRANCIS J MILLER
金额：
$ 36.01万
依托单位：
UNIVERSITY OF IOWA
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-16 至 2010-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7122935
关键词：
NAD(P)H dehydrogenase atherosclerosis blood vessels cytoprotection enzyme activity genetically modified animals glutathione peroxidase laboratory mouse oxidation reduction reaction pathologic process

项目摘要

DESCRIPTION (provided by applicant): Production of reactive oxygen species (ROS) and changes in the cellular redox environment regulate various aspects of cellular function. Previous studies of oxidative stress in vascular disease have focused on ROS generating systems. Little is known, however, regarding effects of the redox state of vascular cells on ROS signaling and cell function in vascular disease. Glutathione (GSH) is the most abundant redox buffer in the cell. The cytosolic enzyme glutathione peroxidase-1 (GPx-1) protects the cell against oxidant stress by utilizing GSH to reduce hydrogen peroxide and lipid peroxides. Oxidative stress, however, will inactivate GPx-1. Although decreased GPx-1 activity is a predictor of cardiovascular events in patients with coronary artery disease, it is not known whether GPx-1 activity directly contributes to the pathophysiology of atherosclerosis. The central hypothesis of this project is that changes in GPx-1 activity in the blood vessel are causally related to the progression of atherosclerosis via alterations in the activity of NADPH oxidase enzymes. The proposed studies are an extension of previous observations made by the investigators of NADPH oxidase-derived ROS in atherosclerosis. First, studies will test the hypothesis that effects of changes in GPx-1 activity on smooth muscle cells are mediated by hvdroperoxide-induced oxidative stress. Proposed studies will test the hypothesis that cellular responses to changes in GPx-1 activity are dependent on the duration of the change in cellular redox. Second, studies are proposed to determine if effects of reduction in GPx-1 activity on smooth muscle cells are mediated by expression of the NADPH oxidase subunits Nox1 and Nox4. Finally, studies are proposed to test the hypothesis that changes of GPx-1 activity in vivo affect progression of atherosclerosis and the expression of Nox enzymes. Information gained from these studies will provide a foundation for additional studies of redox status in vascular disease and potential novel therapeutic strategies to modify the progression of atherosclerosis in patients.

描述（由申请人提供）：活性氧（ROS）的产生和细胞氧化还原环境的变化调节细胞功能的各个方面。先前关于血管疾病中氧化应激的研究主要集中在ROS生成系统上。然而，关于血管疾病中血管细胞的氧化还原状态对 ROS 信号传导和细胞功能的影响知之甚少。谷胱甘肽 (GSH) 是细胞中最丰富的氧化还原缓冲液。胞浆酶谷胱甘肽过氧化物酶-1 (GPx-1) 通过利用 GSH 减少过氧化氢和脂质过氧化物，保护细胞免受氧化应激。然而，氧化应激会使 GPx-1 失活。尽管 GPx-1 活性降低是冠状动脉疾病患者心血管事件的预测因子，但尚不清楚 GPx-1 活性是否直接导致动脉粥样硬化的病理生理学。该项目的中心假设是，血管中 GPx-1 活性的变化通过 NADPH 氧化酶活性的改变与动脉粥样硬化的进展存在因果关系。拟议的研究是研究人员先前对动脉粥样硬化中 NADPH 氧化酶衍生的 ROS 观察结果的延伸。首先，研究将检验以下假设：GPx-1 活性变化对平滑肌细胞的影响是由过氧化氢诱导的氧化应激介导的。拟议的研究将检验以下假设：细胞对 GPx-1 活性变化的反应取决于细胞氧化还原变化的持续时间。其次，建议进行研究以确定 GPx-1 活性降低对平滑肌细胞的影响是否是由 NADPH 氧化酶亚基 Nox1 和 Nox4 的表达介导的。最后，提出研究来检验体内 GPx-1 活性的变化影响动脉粥样硬化进展和 Nox 酶表达的假设。从这些研究中获得的信息将为血管疾病氧化还原状态的进一步研究以及改变患者动脉粥样硬化进展的潜在新治疗策略奠定基础。