Long Term Storage and Precision Dispensing of Precious Liquids

贵重液体的长期储存和精确分配

• 批准号: 7106733
• 负责人: GORDON Ian ROBERTSON
• 金额: $ 10万
• 依托单位: ARCHIVEX, LLC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-09-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7106733
• 关键词: animal genetic material tag; biomedical automation; biomedical registry /referral center; chemical standardization; genetic registry /resource /referral center; human genetic material tag; preservation; technology /technique development; animal genetic material tag; biomedical automation; biomedical registry /referral center; chemical standardization; genetic registry /resource /referral center; human genetic material tag; preservation; technology /technique development

DESCRIPTION (provided by applicant): Integrated disease phenotype and cellular genotype research is advanced by testing hypotheses using stored aqueous samples of DNA, cDNA or other precious liquids from subjects with known characteristics. Many life science laboratories store thousands of such aqueous samples for years or decades, and these researchers rely absolutely on accurate inventory knowledge and known provenance of the samples. The methods used today for storing these precious liquid samples are ad-hoc and prone to contamination and mis-identification. This reduces the productivity and satisfaction of researchers in a variety of ways. The goal of this project is to develop a system for long term storage of precious liquids in standardized containers with machine-readable identification for positive and unambiguous identification of their contents. An automated precision dispenser which does not require pipette intrusion will ensure sample integrity while dispensing microliter aliquots of precious liquid. An interface between the automated dispensing equipment and the laboratory database will maintain accurate inventory control and allow improved experiment planning. This system will greatly increase the efficiency and reduce the error rate of genomic research by ensuring that the precious liquid samples are contamination-free, maintain their concentration levels, and are correctly and positively identified. When completed, the system will be commercialized by marketing it to life-science laboratories, forensic laboratories and hospitals. Technical innovations in this program are the patented design of the standardized storage container; a novel and proprietary means for dispensing 1 ul drops without contamination, and the container identification method and database interface protocols. During Phase 1 of the project, prototypes of the disposable container with machine-readable ID will be designed and built (Specific Aim 1), the proprietary new drop dispensing technology will be developed (Specific Aim 2), and software interface protocols for communicating between the system and the laboratory database will be planned (Specific Aim 3). As each prototype is built, it will be alpha tested in an operating life-science laboratory. During Phase 2, a fully automated system with direct interface to the user laboratory database will be constructed. Public health will directly benefit from the results of the proposed work by reducing or eliminating a major impediment to optimal utilization of precious liquid DNA, cDNA, and other precious liquid source materials which form the bedrock of research linking phenotype and genotype. Compared to present methods, this technology will greatly improve research efficiency by reducing contamination and labor costs in handling samples. The database interface will allow researchers to address interesting outlier samples much more easily during the research process, and permit them to address their complete sample sets more readily when planning experiments.

描述（由申请人提供）：通过使用具有已知特征的受试者的DNA，cDNA或其他贵重液体的储存水性样品来测试假设，从而提出了综合疾病表型和细胞基因型研究。许多数年或几十年来，许多生命科学实验室存储了数千个这样的水性样本，这些研究人员绝对依靠准确的库存知识和样本的已知出处。如今，用于存储这些宝贵液体样品的方法是临时且容易污染和识别。这以多种方式降低了研究人员的生产力和满意度。该项目的目的是开发一个系统，用于在标准化容器中长期存储珍贵的液体，并具有可读机器可读识别，以实现其内容的正面和明确的识别。不需要移液侵入的自动精度分配器将确保样品完整性，同时分配珍贵液体的微晶等分试样。自动分配设备和实验室数据库之间的接口将保持准确的库存控制并允许改进的实验计划。该系统将通过确保宝贵的液体样品不含污染，保持其浓度水平并正确鉴定出来，从而大大提高效率并降低基因组研究的错误率。完成后，该系统将通过将其营销到生命科学实验室，法医实验室和医院进行商业化。该程序中的技术创新是标准化存储容器的专利设计；一种新颖且专有的手段，用于分配1个无污染的液滴，以及容器识别方法和数据库界面协议。在项目的第1阶段，将设计和构建具有机器可读ID的一次性容器的原型（特定的AIM 1），将开发专有的新滴分配技术（特定AIM 2），并计划在系统和实验室数据库之间进行通信的软件接口协议（特定的AIM 3）。在每个原型建造时，它将在操作生命科学实验室中进行α测试。在第2阶段，将构建具有与用户实验室数据库的直接接口的全自动系统。公共卫生将通过减少或消除最佳利用珍贵液体DNA，cDNA和其他构成联系的研究基础质量的宝贵液体DNA，cDNA和其他珍贵液体源材料的主要利用，直接从拟议工作的结果中受益。与当前的方法相比，该技术将通过降低处理样本中的污染和人工成本来大大提高研究效率。数据库界面将使研究人员可以在研究过程中更轻松地解决有趣的离群样本，并允许他们在计划实验时更容易地解决其完整样本集。