Molecular diversity in olfactory wiring specificity

嗅觉线路特异性的分子多样性

• 批准号: 7221402
• 负责人: Maria L Spletter
• 金额: $ 3.83万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7221402
• 关键词: Drosophilidae; RNA; axon; chemoreceptors; dendrites; fingers; in situ hybridization; model; mutant; neurons; olfactions; transcription factor; transfection; Drosophilidae; RNA; axon; chemoreceptors; dendrites; fingers; in situ hybridization; model; mutant; neurons; olfactions; transcription factor; transfection

DESCRIPTION (provided by the applicant): The Drosophila olfactory system presents a great opportunity to understand the general principles of wiring specificity, and how the wiring of neuronal circuits contributes to olfaction. In the Drosophila olfactory system, second order projection neurons (PNs) innervate specific glomeruli in the antennal lobe. We are exploring the molecular mechanisms by which PNs target their dendrites to specific glomeruli. Longitudinals lacking (lola) is a Zn-finger transcription factor identified as a putative regulator of axon guidance that splices to at least 20 unique isoforms. This application will further investigate the function of individual Lola isoforms in PN wiring specificity. The lola locus may prove a useful model to demonstrate the function of molecular diversity at the transcriptional level in wiring specificity. Specifically, I will 1. Perform a systematic expression analysis of individual lola isoforms in the AL using isoform specific RNA in-situ hybridization and immunostaining. 2. Generate new isoform-specific mutants for those isoforms expressed in PNs using homologous recombination, and test their function in PN wiring using MARCM analysis. 3. Generate isoform-specific UAS transgenes to test their ability to rescue isoform-specific mutants using MARCM mediated overexpression.

描述（由申请人提供）：果蝇嗅觉系统提供了一个很好的机会，可以理解接线特异性的一般原则，以及神经元电路的接线如何有助于嗅觉。在果蝇嗅觉系统中，二阶投影神经元（PNS）在触角叶中支配特定的肾小球。我们正在探索PNS将其树突靶向特定肾小球的分子机制。缺乏纵向（LOLA）是一个Zn指转录因子，被确定为轴突指导的推定调节剂，可将至少20个独特的同工型拼接。该应用程序将进一步研究单个LOLA同工型在PN接线特异性中的功能。 LOLA基因座可能证明是一个有用的模型来证明分子多样性的功能 在接线特异性的转录级别上。具体而言，我将1。使用同工型特异性RNA在原位杂交和免疫染色的AL中对单个LOLA同工型进行系统的表达分析。 2。为使用同源重组在PNS中表达的同工型生成新的同工型特异性突变体，并使用MARCM分析测试其在PN接线中的功能。 3。生成同工型特异性的UAS转基因，以测试其使用MARCM介导的过表达来挽救同工型特异性突变体的能力。