The Role of Chromatin Modification During Development

染色质修饰在发育过程中的作用

• 批准号: 7008141
• 负责人: Johnathan R. Whetstine
• 金额: $ 5.04万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7008141
• 关键词: Caenorhabditis elegans; RNA interference; amidohydrolases; chromatin; chromatin immunoprecipitation; developmental genetics; embryogenesis; enzyme activity; enzyme mechanism; gene induction /repression; genetic regulation; microarray technology; polymerase chain reaction; postdoctoral investigator

DESCRIPTION (provided by applicant): Chromatin modification has recently emerged as a novel and important mechanism in the regulation of gene expression. However, the impact that chromatin modifiers and the resulting modifications have on molecular networks establishing proper cell-fate and development in multi-cellular organisms are just beginning to emerge. In order to better understand this question, this NRSA will use the C. elegans as an in vivo system to investigate the role of HDA-1 (histone-deacetylase 1) during early embryogenesis by using molecular, biochemical, and genomic approaches. Since HDA-1 is crucial for proper cell-fate determination, the identification of direct HDA-1 target genes and the impact that this protein has on the chromatin environment will be fundamental in understanding the mechanistic role of HDA-1. Based on these exciting findings, new models are likely to emerge about the in vivo function and mechanism of action of histone deacetylases in the development of a multi-cellular organism.

描述（由申请人提供）： 染色质修饰最近已成为基因表达调控中一种新颖且重要的机制。然而，染色质修饰剂和由此产生的修饰对建立正确细胞命运和多细胞生物发育的分子网络的影响才刚刚开始显现。 为了更好地理解这个问题，本次 NRSA 将使用秀丽隐杆线虫作为体内系统，通过分子、生化和基因组方法研究 HDA-1（组蛋白脱乙酰酶 1）在早期胚胎发生过程中的作用。由于 HDA-1 对于正确的细胞命运决定至关重要，因此直接 HDA-1 靶基因的鉴定以及该蛋白对染色质环境的影响对于理解 HDA-1 的机制作用至关重要。基于这些令人兴奋的发现，可能会出现关于组蛋白脱乙酰酶在多细胞生物体发育中的体内功能和作用机制的新模型。