Steroid modulation of a rhythmic vocal pattern generator

节奏声音模式发生器的类固醇调制

• 批准号: 7071065
• 负责人: MICHELE C ZEE
• 金额: $ 4.88万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-16 至 2008-06-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7071065
• 关键词: Osteichthyes; alternatives to animals in research; animal communication behavior; behavioral /social science research tag; bioperiodicity; developmental neurobiology; electrophysiology; gender difference; hormone receptor; hormone regulation /control mechanism; motor neurons; nuclear receptors; postdoctoral investigator; seasons; sex behavior; steroid hormone; vocalization; voltage /patch clamp

DESCRIPTION (provided by applicant): Vocal communication is essential for courtship among many vertebrates. Studies in teleost fish provide considerable evidence that vocalizations are controlled by distinct neural circuits which show seasonal and sex differences in response to circulating levels of steroid hormones. Prior in vivo work has also shown that steroids can induce rapid changes in 'fictive vocalization.' This application will focus on the teleost vocal pattern generator located in the hindbrain-spinal region since it provides the simplest vertebrate preparation for determining how steroid hormones modulate the properties of pacemaker and motor neurons that lead to vocalization within a social context. An electrophysiological approach will be used to describe steroid-induced changes in vocal patterning at the level of intrinsic membrane properties of these identified neurons with the development of an in vitro preparation. The central goal of this research is to understand how steroid hormones modulate the production of highly stereotyped sounds for vocal communication by mediating changes in neuronal membrane properties via both rapid, non-genomic mechanisms and longer acting mechanisms mediated by nuclear hormone receptors.

描述（由申请人提供）：声音交流对于许多脊椎动物的求偶至关重要。对硬骨鱼的研究提供了大量证据，表明发声是由不同的神经回路控制的，这些神经回路对类固醇激素循环水平的反应表现出季节性和性别差异。先前的体内研究还表明，类固醇可以引起“虚构发声”的快速变化。该应用程序将重点关注位于后脑脊髓区域的硬骨鱼发声模式发生器，因为它提供了最简单的脊椎动物准备，用于确定类固醇激素如何调节起搏器和运动神经元的特性，从而在社交环境中发声。随着体外制剂的开发，将使用电生理学方法在这些已识别神经元的内在膜特性水平上描述类固醇诱导的声音模式变化。这项研究的中心目标是了解类固醇激素如何通过快速、非基因组机制和核激素受体介导的长效机制来介导神经元膜特性的变化，从而调节用于声音交流的高度刻板声音的产生。