Assessment of Muscle Vascular Disease with Diffuse Light

用漫射光评估肌肉血管疾病

• 批准号: 7022601
• 负责人: Guoqiang Yu
• 金额: $ 18.64万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-15 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7022601
• 关键词: arm; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; blood flow measurement; cardiovascular imaging /visualization; claudication; clinical research; diagnosis design /evaluation; foot; hemodynamics; human subject; ischemia; leg; magnetic resonance imaging; microcirculation; musculoskeletal circulation; noninvasive diagnosis; optics; oxygen consumption; oxygen transport; peripheral blood vessel disorder; spectrometry

DESCRIPTION (provided by applicant): Individuals with chronic critical limb ischemia (CCLI) suffer from microvascular disease that negatively impacts the success of interventions for limb salvage. Current diagnostic tools are largely limited to assessing blood flow within large vessels. The ultimate goal of this project is to develop a non-invasive optical probe for imaging of microcirculation and tissue metabolism in patients with CCLI. SPECIFIC AIMS: (1) Modify a multi-modal optical instrument for integration with Arterial Spin Labeling MRI. (2) Crossvalidation of optical blood flow and MRI perfusion in human muscles. (3) Quantify muscle blood flow, oxygenation, and oxygen consumption in patients with CCLI, and correlate these variables with accepted vascular testing parameters. RESEARCH DESIGN AND METHODS: The lack of portable, noninvasive technologies for monitoring of both blood flow and oxygenation in the deep microcirculation has led us to develop a hybrid optical probe combining, for the first time, diffuse correlation spectroscopy (DCS) for studies of tissue blood flow with diffuse reflectance spectroscopy (DRS) for studies of tissue oxygenation. DRS has been validated in a few studies of deep muscle tissues. The same cannot be said for DCS. The proposed simultaneous MRI/Optical investigation of reactive hyperemia in normal human muscle will validate DCS, and explore possible uses of the combined information for accurate analysis of tissue physiology. For this purpose, a new non-metallic optical probe will be designed and tested to adapt to the MRI. Correlations of the two simultaneous measurements will be investigated. After validating the DCS flow, the first simultaneous all-optical measurements of blood flow and oxygenation will be conducted in the patients with CCLI during cuff occlusion or exercise. Multiple hemodymamic indices will be constructed from these measured variables and then be correlated with accepted vascular testing parameters such as the Ankle- Brachial Index or Toe-Brachial Systolic Index. RELEVANCE: Current diagnostic techniques are inadequate to offer necessary information for the patients with chronic critical limb ischemia when determining limb, salvage procedures or the extent of amputation, causing a serious health concern. The proposed, noninvasive, portable, and inexpensive optical technology may fill in this gap by providing multiple hemodynamic information and by enabling continuous measurements at the bed-side and in ambulatory care.

描述（由申请人提供）：患有慢性严重肢体缺血（CCLI）的个体患有微血管疾病，这会对保肢干预措施的成功产生负面影响。目前的诊断工具很大程度上仅限于评估大血管内的血流。该项目的最终目标是开发一种非侵入性光学探针，用于对 CCLI 患者的微循环和组织代谢进行成像。具体目标： (1) 修改多模态光学仪器以与动脉旋转标记 MRI 集成。 (2) 人体肌肉中光血流和 MRI 灌注的交叉验证。 (3) 量化 CCLI 患者的肌肉血流量、氧合和耗氧量，并将这些变量与公认的血管测试参数相关联。研究设计和方法：由于缺乏便携式、无创技术来监测深部微循环中的血流和氧合，我们开发了一种混合光学探针，首次结合了漫相关光谱（DCS）来研究组织使用漫反射光谱 (DRS) 进行血流研究，用于研究组织氧合。 DRS 已在一些深层肌肉组织的研究中得到验证。 DCS 则不然。所提出的对正常人体肌肉反应性充血的同步 MRI/光学研究将验证 DCS，并探索组合信息的可能用途，以准确分析组织生理学。为此，将设计并测试一种新的非金属光学探头以适应MRI。将研究两个同时测量的相关性。验证 DCS 血流后，将在袖带闭塞或运动期间对 CCLI 患者进行血流和氧合的首次同步全光学测量。将根据这些测量变量构建多个血液动力学指数，然后与公认的血管测试参数（例如踝肱指数或趾肱收缩指数）相关。相关性：当前的诊断技术不足以为慢性严重肢体缺血患者在确定肢体、抢救程序或截肢范围时提供必要的信息，从而造成严重的健康问题。所提出的非侵入性、便携式且廉价的光学技术可以通过提供多种血流动力学信息并在床边和门诊护理中实现连续测量来填补这一空白。