Retroviral Transduction Using Acoustic Waves

使用声波进行逆转录病毒转导

基本信息

批准号：
7047759
负责人：
Pin Wang
金额：
$ 19.05万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-06-01 至 2007-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7047759
关键词：
CD34 molecule Retroviridae Vesiculovirus bioengineering /biomedical engineering biotechnology cell line gene therapy genetic transduction green fluorescent proteins hematopoietic stem cells host organism interaction technology /technique development therapy design /development transfection /expression vector ultrasound virus protein

项目摘要

DESCRIPTION (provided by applicant): Among all types of viruses currently used in clinical trials, those based on the murine leukemia virus retroviral vectors are the most frequently used, because of its capability of stable gene integration to the chromosomes of target cells. However, retroviruses can transducer only actively dividing cells. With the relatively short half-life of retroviral particles, the probability of infectious retroviruses encountering target cells is very low because they move in a random Brownian motion. Even if they can get close to the target cells, there is the repulsion force generated from the interaction between negatively charged retrovirus envelope and cell membrane. To overcome these limitations, physicochemical approaches used to increase cell-virus contact such as addition of polycation, flow-through of virus-containing medium, spinoculation, and magnetic field have been studied and showed improved results. However, the aforementioned transduction studies were focused on anchorage-dependent cells and not easy for large-scale settings. In this study, GFP-encoding VSV-G pseudotyped retrovirus vector, which is less fragile than the un-modified retroviral vector, will be used to infect cytokine-dependent hematopoietic cell lines and primary CD34+ hematopoietic cells within ultrasonic standing wave fields generated by piezoelectric transducer and reflector. Various cytokine cocktails will be employed to stimulate the hematopoietic cells entering cell cycle and thus enhance the retroviral transduction. According to the primary acoustic radiation force and drag force, the suspended cells can be estimated to arrive at the pressure nodal planes on the 1 -2 second time scale. We speculate that the first arrived and agglomerated cells may play a role on nucleating collection of the 100 nm-sized retroviruses at the nodal planes. Several design and operating approaches are proposed to decrease the undesired acoustic and thermal streaming which might prevent the aggregation of particulates with diameter around 100 nanometer such as retroviruses. The significance of the proposed research is the engineering approach (i.e., ultrasonic standing waves fields) could be harnessed to enhance the retroviral gene delivery efficiency to hematopoietic stem/progenitor cells, which are not easy to be retrovirally transduced with current medical approaches. The success of this proposed study will provide an innovative method to the field of gene therapy.

描述（由申请人提供）：在临床试验中当前使用的所有类型的病毒中，基于鼠白血病病毒逆转录病毒载体的病毒是最常用的，因为它具有稳定基因整合到靶细胞染色体的能力。但是，逆转录病毒只能换能细胞。由于逆转录病毒颗粒的半衰期相对较短，遇到靶细胞的感染性逆转录病毒的可能性非常低，因为它们以随机的布朗运动运动。即使它们可以接近靶细胞，也存在负电荷逆转录病毒包膜和细胞膜之间的相互作用产生的排斥力。为了克服这些局限性，已经研究了用于增加细胞病毒接触的物理化学方法，例如添加多阳离子，含病毒培养基的流动性，脊柱型和磁场的流通量并显示出改善的结果。然而，上述转导研究集中在锚定依赖性细胞上，对于大规模设置并不容易。在这项研究中，GFP编码的VSV-G Pseudotypy逆转录病毒载体比未修饰的逆转录病毒载体不那么脆弱，将用于感染超声波场中的细胞因子依赖性造血细胞系和原发性CD34+造血场+造血场中由piezozoeleclectric和pieDorcrectric的超声波场中产生的。将使用各种细胞因子鸡尾酒来刺激进入细胞周期的造血细胞，从而增强逆转录病毒转导。根据原发性声学辐射力和阻力力，可以估计悬浮的细胞在1 -2秒尺度上到达压力淋巴结。我们推测，第一个到达的细胞可能会在淋巴结平面的100 nm大小逆转录病毒的成核收集中发挥作用。提出了几种设计和操作方法，以减少不需要的声学和热流，这可能会阻止直径约100纳米（如逆转录病毒）的直径聚集。拟议的研究的重要性是可以利用工程方法（即超声波站领域）提高逆转录病毒基因递送效率到造血干/祖细胞，这些细胞并不容易通过当前的医疗方法逆转录病毒。这项拟议的研究的成功将为基因治疗领域提供创新的方法。