A New Method for Retinal Imaging

视网膜成像的新方法

• 批准号: 6936507
• 负责人: ALAN D MARMORSTEIN
• 金额: $ 15.05万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-07 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6936507
• 关键词: aging; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; eye; eye disorder diagnosis; eye fundus photography; fluorescence; human tissue; macular degeneration; patient oriented research; postmortem; retinal pigment epithelium; retinography; aging; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; eye; eye disorder diagnosis; eye fundus photography; fluorescence; human tissue; macular degeneration; patient oriented research; postmortem; retinal pigment epithelium; retinography

DESCRIPTION (provided by applicant): Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in the western world affecting nearly 30% of those over the age of 75. The only accepted risk factors for AMD are age, race, and smoking. AMD alters the quality of life of those affected by causing a debilitating loss of central vision. Currently diagnosis of AMD at its early stages can be difficult. This has led to problems in the development of drugs to treat the disease since clinical trials must rely on following the slow progression of the disease in individuals that are already demonstrating some loss of visual acuity. We have recently found that Bruch's membrane and sub-retinal pigment epithelium (RPE) deposits characteristic of AMD exhibit a unique autofluorescence spectrum that can be excited with light between 360 and 490nm. When compared with the intensity of autofluorescence elicited from RPE lipofuscin, these emissions were significantly elevated in eyes from donors with AMD versus healthy donor eyes. The change in the ratios of these emissions (Bruch's membrane and sub-RPE deposits vs. lipofuscin) correlating with disease suggests that the same principles that allow quantitative microscopy using fluorescence ratiometry could be applied to fundus photography. The goal of the work outlined in this proposal is to design, build, and test a device for fundus fluorescence ratiometry, and to determine the efficacy of this device in the early diagnosis of retinal lesions that are risk factors for AMD. To accomplish this we will perform a series of 3 specific aims. In the first aim we will modify a standard clinical fundus camera for fundus fluorescence ratiometry. In specific aim 2 we will determine ideal excitation and emission wavelengths for fundus fluorescence ratiometry. This will be accomplished by testing a variety of excitation and emission wavelengths using the modified camera on postmortem human donor eyes. In specific aim 3 we will determine whether fundus fluorescence ratiometry shows promise for the diagnosis of lesions associated with AMD. This will be accomplished using eyes from donors with AMD. Specifically we will test the efficacy of this device in identifying basal deposits. Basal deposits are specific retinal lesions that are not visible during a clinical fundus exam. One form of basal deposit, basal linear deposits, are more common in AMD eyes than age-matched controls. A positive outcome to this study would warrant future clinical trials.

描述（由申请人提供）：与年龄相关的黄斑变性（AMD）是西方世界不可逆转的失明的主要原因，影响了75岁以上的人中的近30％。AMD唯一接受的危险因素是年龄，种族和吸烟。 AMD通过导致中央视力丧失的人的生活质量改变了人们的生活质量。 目前，AMD在早期阶段的诊断可能很困难。 这导致了治疗该疾病的药物发展问题的问题，因为临床试验必须依靠已经显示出视力丧失的个体的疾病进展缓慢。 我们最近发现，AMD的Bruch膜和视网膜色素上皮（RPE）沉积物具有独特的自动荧光光谱，可以用360至490nm之间的光激发。 与RPE脂肪霉素引起的自动荧光强度相比，这些排放的眼睛与健康供体眼相对于健康供体眼睛的供体的眼睛显着升高。 这些排放率（BRUCH的膜和亚RPE沉积物与脂肪霉素）的比率的变化与疾病相关，这表明可以将使用荧光比计量法的定量显微镜的相同原理应用于底面摄影。 该提案中概述的工作的目的是设计，构建和测试用于底面荧光比计量法的设备，并确定该设备在早期诊断视网膜病变中的功效，这是AMD的危险因素。为了实现这一目标，我们将执行一系列特定目标。 在第一个目的中，我们将修改标准的临床眼底摄像头，以用于底面荧光比法。 在特定的目标2中，我们将确定基底荧光比计的理想激发和发射波长。 这将通过使用后捐赠者眼睛上的改良摄像头测试各种激发和发射波长来实现。 在特定目标3中，我们将确定底眼荧光比计量法是否显示出与AMD相关病变的诊断的希望。 这将使用AMD的捐助者的眼睛来完成。 具体而言，我们将测试该设备在识别基础沉积物方面的功效。 基础沉积是在临床眼底检查期间不可见的特定视网膜病变。 基础沉积物的一种基础线性沉积物的一种形式在AMD眼中比年龄匹配的对照更常见。 这项研究的积极结果将值得将来的临床试验。