Core--Demonstration

核心--演示

• 批准号: 6952180
• 负责人: GREGORY L STAHL
• 金额: $ 8.84万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6952180
• 关键词: biomedical facility; chemical structure function; dental disorder chemotherapy; disease /disorder model; drug screening /evaluation; flow cytometry; genetically modified animals; laboratory mouse; laboratory rabbit; neutrophil; periodontium disorder

Studies in the area of periodontal disease have defined the crucial factors involved in the inflammatory process including; 1) the role of neutrophils; 2) the role of lipid mediators; 3) identification of bacterial processes in amplifying the inflammatory condition; and 4) the role of the hosts innate and humoral immune systems. In support of projects 1-4, and to optimize, centralize and facilitate the discovery of novel, topically active anti- inflammatory and anti-PMN analogues of naturally occurring lipid mediators; this Core will provide and apply established in vivo animal, cellular and organ models, as well as carry out in vitro assays to: a) assist in the discovery of structure/function relationships of novel, topically occurring anti-PMN and anti-inflammatory compounds generated in Projects 1-4 and establish their actions in vivo in wild type and genetically altered rabbits. b) provide flow cytometric analysis of cells from Project 1-4. c) provide PMN functional assays in vitro and in vivo. d) provide and develop animal and cellular models/assays for the evaluation of novel therapeutics in periodontitis. Providing a centralized location of these routine tasks to this Core will eliminate costly duplication of these assays/models within the other projects. This Core will optimize productive interactions and resource utilization and provide the other projects with efficient and timely access to novel. Prioritization of the provide the other projects with efficient and timely access to novel cell and in vivo assays. Prioritization of the assays/models will be accomplished through individual meetings between Core D's principal investigators and the Program Project principal investigators and during monthly meetings of the Program Project team. Centralization of these models and assays will facilitate the demonstration and the effectiveness of these novel therapeutics in periodontal disease.

牙周疾病领域的研究定义了炎症过程中涉及的关键因素，包括： 1）中性粒细胞的作用； 2）脂质介质的作用； 3）在扩增炎症状态下鉴定细菌过程； 4）宿主先天和体液免疫系统的作用。支持项目1-4，并优化，集中和促进自然存在的脂质介质的新型，主动抗炎和抗PMN类似物的发现；该核心将提供并应用已建立的体内动物，细胞和器官模型，并在体外测定中进行：a）有助于发现项目1-4中新型的，局部发生的抗PMN和抗炎性化合物的结构/功能关系，并在野生类型和遗传上改变兔子中的Vivo中产生的抗炎化合物。 b）提供项目1-4细胞的流式细胞仪分析。 c）在体外和体内提供PMN功能测定。 d）提供并开发动物和细胞模型/测定法，以评估牙周炎的新疗法。将这些日常任务的集中位置提供给该核心将消除其他项目中这些测定/模型的昂贵重复。该核心将优化生产性互动和资源利用，并为其他项目提供高效，及时的小说访问。优先考虑其他项目可以有效，及时访问新细胞和体内测定。分析/模型的优先级将通过Core D的主要研究人员与计划项目首席研究人员以及计划项目团队的每月会议之间的个人会议来完成。这些模型和测定的集中化将有助于这些新型治疗剂在牙周疾病中的示范和有效性。