Early cellular immune responses to EBV

针对 EB 病毒的早期细胞免疫反应

• 批准号: 6967184
• 负责人: SUMITA BHADURI-MCINTOSH
• 金额: $ 12.58万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6967184
• 关键词: B lymphocyte; BCL2 gene /protein; Epstein Barr virus; cell transformation; cellular immunity; clinical research; cysteine endopeptidases; cytokine; cytokine receptors; cytotoxicity; diagnosis design /evaluation; exocytosis; flow cytometry; helper T lymphocyte; human subject; immune response; immunocytochemistry; latent virus infection; lymphocyte; mitochondria; mixed tissue /cell culture; monoclonal antibody; monocyte; recombinant proteins; virus antigen

DESCRIPTION (provided by applicant): This proposal outlines a 5-year training program for the development of an academic career in Pediatric Infectious Disease. The candidate has completed residency training in Pediatrics, Ph.D. training in Molecular and Cell Biology, and fellowship training in Infectious Disease. She is now in the midst of enhancing her scientific skills through a unique blend of interdepartmental resources and expertise. The candidate's goal is to develop a command of cellular immunology as it relates to viruses by integrating EBV immunology research and a structured curriculum to enhance scientific knowledge in the fields of both virology and cellular immunology. Dr. George Miller, a renowned EBV virologist will mentor the candidate's scientific development. To enhance the experience, an advisory committee of highly respected immunologists studying diverse aspects of cellular immunology will provide scientific and career guidance. The candidate's research will examine the immune responses in the early stages of primary EBV infection and in the early stages of control of outgrowth of latently infected B cells in EBV-immune individuals. These questions will be addressed using a relevant and unique model experimental system that the candidate has developed. The goals are: 1. To identify the cells important in the initial response to EBV-LCL during primary infection and for the prevention of outgrowth of latently infected B cells in EBV seropositive individuals. 2. To determine if the responding cells are cytotoxic to LCL and characterize the mechanism of cell killing. 3. To experimentally modulate the composition and nature of the immune response by using exogenous molecules. This study will, for the first time, allow glimpses into the earliest interactions between the immune system and EBV without modification or amplification of the immune response. It is also expected to enhance the understanding of EBV lymphoproliferative diseases and have potential diagnostic and therapeutic implications.

描述（由申请人提供）：该提案概述了一个为期 5 年的儿童传染病学术职业发展培训计划。候选人已完成儿科住院医师培训，博士。分子和细胞生物学培训以及传染病研究金培训。她现在正在通过跨部门资源和专业知识的独特结合来提高她的科学技能。 候选人的目标是通过整合 EBV 免疫学研究和结构化课程来提高对与病毒相关的细胞免疫学的掌握，以增强病毒学和细胞免疫学领域的科学知识。著名 EB 病毒学家 George Miller 博士将指导候选人的科学发展。为了增强体验，由研究细胞免疫学各个方面的备受尊敬的免疫学家组成的咨询委员会将提供科学和职业指导。 该候选人的研究将检查 EBV 免疫个体中原发性 EBV 感染早期阶段以及控制潜伏感染 B 细胞生长的早期阶段的免疫反应。这些问题将使用候选人开发的相关且独特的模型实验系统来解决。目标是： 1. 鉴定在原发感染期间对 EBV-LCL 的初始反应以及预防 EBV 血清阳性个体中潜伏感染 B 细胞生长的重要细胞。 2. 确定响应细胞是否对 LCL 具有细胞毒性并表征细胞杀伤机制。 3. 使用外源分子通过实验调节免疫反应的组成和性质。这项研究将首次让我们得以一睹免疫系统与 EBV 之间最早的相互作用，而无需修改或放大免疫反应。它还有望增强对 EBV 淋巴细胞增殖性疾病的了解，并具有潜在的诊断和治疗意义。