Biophysical Modeling of Solute Transport in Human IVD

人体 IVD 中溶质转运的生物物理模型

• 批准号: 6924392
• 负责人: Weiyong Gu
• 金额: $ 29.44万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6924392
• 关键词: backache; biological transport; biomechanics; biophysics; density; electrical conductance; electrochemistry; human tissue; intervertebral disk; mathematical model; mechanical stress; membrane permeability; nutrient bioavailability; oxygen tension; postmortem; spinal disk injury

DESCRIPTION (provided by applicant): Low back pain is a major socio-economic concern in this country. Although the exact cause for low back pain is unclear, the degenerative changes of the intervertebral disc (IVD) have been implicated as a possible primary etiologic factor. Poor nutritional supply is believed to be one of the mechanisms for disc degeneration. Due to the unique composition and structure of the materials and the complexity of the mechano-electrochemical coupling phenomena in IVD tissues, there is a lack of knowledge on transport properties of human IVDs or appropriate theoretical models for investigating nutrient transport in IVD systematically. The purpose of this application is to determine the transport properties and the constitutive models for these properties in human IVDs required for the development of a new mechano-electrochemical transport theory and finite element model in a subsequent grant application. The broad, long-term objectives of this project are to (1) elucidate the etiology of disc degeneration, (2) help develop strategies for restoring tissue function or retarding further disc degeneration, and (3) develop novel, less-invasive diagnostic tools for disc degeneration. In this research, we will determine the mechanical, physicochemical, and transport properties of human lumbar IVD tissues (Specific Aims #1-#5) and develop and validate new constitutive models for transport properties (Specific Aims #6 & #7). New technologies, based on mechano-electrochemical principles, will be developed for testing transport properties of IVD tissues, including strain-dependent hydraulic permeability, strain-dependent fixed charge density, strain-dependent electrical conductivity, and strain-dependent diffusivity of ions and nutrients (oxygen and glucose) in human lumbar discs. These properties will be correlated to the composition of the tissue, and will be used in developing the new constitutive theories in the research of nutritional supply in human IVDs. The advance in theory, knowledge on material properties, and techniques will have a significant impact on understanding the etiology of disc degeneration as well as on other areas of research, such as drug delivery in biological tissues. A plan for sharing and disseminating the theory, techniques, and data developed in this research is also included.

描述（由申请人提供）：腰痛是该国的主要社会经济问题。尽管尚不清楚腰痛的确切原因，但椎间盘（IVD）的退化性变化已被视为可能的主要病因。据信营养供应不良是椎间盘变性的机制之一。由于材料的独特组成和结构以及IVD组织中机械电化学偶联现象的复杂性，因此缺乏对人IVD的运输特性或适当的理论模型的知识，用于系统地研究IVD的营养转运。本应用的目的是在随后的授予应用中确定开发新机械电气化学传输理论和有限元模型所需的人类IVD中这些特性的传输属性和本构模型。该项目的广泛，长期目标是（1）阐明椎间盘变性的病因，（2）有助于制定恢复组织功能或延迟进一步的椎间盘变性的策略，以及（3）开发新型，侵入性较低的诊断工具，以使椎间盘退化。在这项研究中，我们将确定人类腰部IVD组织的机械，物理化学和运输特性（特定目的＃1-＃5），并开发和验证新的运输特性组成模型（特定目标＃6＆＃7）。将开发基于机械电化学原理的新技术，以测试IVD组织的转运性能，包括依赖性液压渗透性，依赖性固定电荷密度，依赖性应变依赖性的电导率以及人类lumbar lumbar dists的离子和营养素的应变依赖性扩散率。这些特性将与组织的组成相关，并将用于开发人类IVD营养供应研究的新构成理论。理论上的进步，有关材料特性和技术的知识将对理解椎间盘退化的病因以及其他研究领域（例如生物组织中的药物递送）产生重大影响。还包括一项共享和传播本研究中开发的理论，技术和数据的计划。