BRAIN PROTON MAGNETIC RESONANCE SPECTROSCOPY OF CANAVAN DISEASE

卡纳万病的脑质子磁共振波谱分析

• 批准号: 6657662
• 负责人: ZHIYUE J WANG
• 金额: $ 22.55万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6657662
• 关键词: Mammalia; bioimaging /biomedical imaging; biomedical resource; building /facility design /renovation; female; health care; human subject; magnetic resonance imaging; male; medical rehabilitation related tag; musculoskeletal system; prosthesis

Microamperage electrical stimulation ( MENS) is a modality widely used by physical therapists for pain relief and tissue repair. Manufacturers of MENS units have claimed that increased ATP levels result from these stimulation units, though the mechanism of action is unknown and may be dependent on various stimulation paradigms. Studies have been done which establish the link between MENS and ATP synthesis in rat skin (Cheng, et. al., 1982). However, there is a dearth of information on the effect of MENS on ATP in human tissue, despite the FDA approval of MENS units and the wide use of these units in rehabilitative therapy. The purpose of this study is to examine the effect of MENS on muscle metabolism in the human masseter using Magnetic Resonance Spectroscopy (MRS). MRS can objectively evaluate the efficacy of MENS using phosphorous metabolite spectra to measure Pi, Phosphocreatine, and ATP peak areas and ratios. Temporomandibular joint disorder/dysfunction (TMJ) patients will be used in this study to help determine if the MENS units increase the rate of ATP production (which would be expected to aid in tissue repair and pain relief) in damaged or weak tissue. We hypothesize that MENS stimulation will change muscle pH and consumption of phosphorous containing compounds in the TMJ cohort but will not affect the normal population. The pilot study includes both normal and TMJ patients with varying parameters of stimulation to detect optimal levels of stimulation. Once completed, a double blind study will proceed using MRS on normal and TMJ patients with consistent stimulation parameters to measure muscle metabolite levels prior to and during exposure to MENS. Coils will be placed on both masseter muscles in order to measure changes in the metabolite levels in each muscle. Pilot data on 18 normal subjects has not shown ATP level changes with various stimulation parameters, which is in keeping with our hypothesis.

微安培电刺激 (MENS) 是一种广泛应用的治疗方式 物理治疗师使用它来缓解疼痛和修复组织。 MENS 装置的制造商声称，ATP 水平增加 尽管作用机制是这些刺激单元的结果 未知，可能取决于各种刺激范例。 已经完成的研究确立了 MENS 和 ATP 之间的联系 大鼠皮肤中的合成（Cheng 等人，1982）。 然而，有一个 缺乏关于 MENS 对人体组织 ATP 影响的信息， 尽管 FDA 批准了 MENS 装置并广泛使用这些装置 在康复治疗中。 本研究的目的是检验 MENS 对 使用磁共振研究人类咬肌的肌肉代谢 光谱学（MRS）。 MRS可以客观评价MENS的疗效 使用磷代谢物光谱来测量 Pi、磷酸肌酸、 和 ATP 峰面积和比率。 颞下颌关节 本研究将使用颞下颌关节紊乱/功能障碍 (TMJ) 患者来帮助 确定 MENS 单位是否会提高 ATP 生成率（其中 预计有助于组织修复和缓解疼痛） 或组织薄弱。 我们假设 MENS 刺激将会改变 肌肉 pH 值和含磷化合物的消耗 但颞下颌关节群组不会影响正常人群。 该试点研究包括正常患者和患有 TMJ 疾病的患者 改变刺激参数以检测最佳水平 刺激。 一旦完成，将使用双盲研究进行 正常和颞下颌关节患者的 MRS 刺激参数一致 测量暴露前和暴露期间的肌肉代谢水平 男士。 线圈将放置在两侧咬肌上，以便 测量每块肌肉代谢水平的变化。 试点数据 18 名正常受试者的 ATP 水平未显示出随各种因素的变化 刺激参数，这与我们的假设一致。