CORE--CELL BIOLOGY SUPPORT

核心--细胞生物学支持

• 批准号: 7078633
• 负责人: FRANCIS W. LUSCINSKAS
• 金额: $ 14.13万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7078633
• 关键词: animal tissue; biomedical facility; cell line; embryo /fetus tissue /cell culture; human tissue; tissue /cell culture; vascular endothelium

For the past 24 years this Core has provided researchers within this Program Project with a wide variety of well characterized cultured cells from both vascular and non-vascular sources. The personnel within this core have worked closely with researchers to develop new cell culture systems as needed to facilitate the scientific goals of the Program. In the last project period, this has included, for example, further development of methodologies for the isolation of murine lung and heart endothelium, as well as isolation of purified T cell subsets (CD8+ Tc1 and Tc2; CD4+ Th1 and Th2 cells) by immunobeads, which formed the basis of in vivo and in vitro models developed in other subprojects. Core Unit A will continue in its efforts to minimize cost duplication in this Program Project and to provide quality control testing of all key biological reagents used in tissue cultures. These products include fetal bovine serum, endothelial cell growth factors, matrix proteins such as attachment factors and enzymes. All products are routinely tested for their efficacy by core personnel, and then a price quotation is negotiated with three major suppliers, and purchased in bulk quantities for use by the program researchers. This plan has been in place for the past 15 years and has insured that cell cultures derived within this core are maintained in a consistent manner from their initial isolation from tissues through to their experimental use by the Program Project investigators. The core will continue to provide training in general cell culture methodologies and sterile technique to new program personnel (e.g., technicians, medical and graduate students, research fellows), as well as train personnel in techniques and procedures necessary for the safe handling, decontamination and disposal of biohazardous materials as well as propagation and isolation of adenovirus recombinant vectors. Core A will continue to undertake the maintenance of embryonic stem cell lines and additional cell culture materials that are required for gene targeting in mice as during the previous 10 years.

在过去的24年中，该核心为该计划项目中的研究人员提供了来自血管和非血管来源的各种特征性培养细胞。该核心中的人员与研究人员紧密合作，根据需要开发新的细胞培养系统，以促进该计划的科学目标。在最后一个项目期间，例如，这包括进一步开发用于隔离鼠肺和心脏内皮的方法，以及通过免疫片的隔离纯化的T细胞子集（CD8+ TC1和TC2; CD4+ Th1和Th2细胞）通过ImmunoBeads的隔离，这是Immunobead的隔离，这形成了Invivo和In Vitro模型的基础，并在其他subpropross中形成了基础。核心单元A将继续努力，以最大程度地减少该计划项目的成本重复，并提供对组织培养物中使用的所有关键生物试剂的质量控制测试。这些产品包括胎牛血清，内皮细胞生长因子，基质蛋白，例如附着因子和酶。所有产品 经常通过核心人员对其有效性进行测试，然后与三个主要供应商进行了价格报价，并大量购买了该计划研究人员的使用。在过去的15年中，该计划已经制定了，并保证了该核心内部的细胞培养物以一致的方式从组织到程序项目研究者的实验使用中保持一致的方式。核心将继续为新的计划人员（例如技术人员，医学和研究生，研究研究员）以及在安全处理，净化和进行安全处理所需的技术和程序中提供一般细胞培养方法和无菌技术的培训。 处理生化材料以及腺病毒重组载体的传播和隔离。核心A将继续进行胚胎干细胞系的维持和与前10年一样，基因靶向基因所需的其他细胞培养物。