Amygdala and prefrontal control of the rhinal cortices

杏仁核和鼻皮质的前额叶控制

基本信息

批准号：
6896291
负责人：
DENIS PARE
金额：
$ 30.83万
依托单位：
RUTGERS THE STATE UNIV OF NJ NEWARK
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-06-01 至 2010-05-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The rhinal cortices (perirhinal areas 35-36 and entorhinal cortex, EC) play a critical role in high-order perceptual/mnemonic functions and constitute the main route for impulse traffic in and out of the hippocampus. However, there is a discrepancy between anatomical and physiological data about this network. Indeed, tracing studies indicate that the perirhinal cortex forms strong reciprocal connections with the neocortex and EC. In contrast, physiological findings indicate that perirhinal transmission of neocortical and entorhinal inputs occur with an extremely low probability. The general objectives of this proposal are: (A) to shed light on the inhibitory mechanisms that limit impulse traffic through the rhinal cortices and (B) to identify the afferents that allow the rhinal cortices to overcome this inhibition, focusing on inputs from the medial prefrontal cortex (mPFC) and amygdala. To these ends, we will: (1) identify the transmitter and synaptic targets of neocortical and entorhinal axons to areas 35, 36 and EC using anterograde (or retrograde) tracer injections in the temporal neocortex or particular rhinal fields coupled to immunocytochemistry at the electron microscopic level. (2) determine the effect of neocortical and entorhinal stimuli on rhinal neurons recorded in vitro with the whole cell patch method and in vivo with sharp micropipettes. (3-4) determine how the mPFC and amygdala affect impulse traffic across the rhinal cortices. This will be studied using a combination of anatomical and physiological experiments. Anatomical experiments will require anterograde tracer injections restricted to particular regions of the mPFC and amygdala, and the same methods as in (1). Physiological experiments will involve inverse dialysis of picrotoxin in mPFC or amygdala and multiple simultaneous extra- and intracellular neuronal recordings of rhinal neurons in vivo. Since the rhinal cortices are primarily damaged during early stages of Alzheimer's disease, this basic research program may improve our understanding of memory disorders.

描述（由申请人提供）：鼻皮质（鼻周区域 35-36 和内嗅皮质，EC）在高阶知觉/记忆功能中发挥着关键作用，并构成了进出海马体的冲动交通的主要路线。然而，该网络的解剖学和生理学数据之间存在差异。事实上，追踪研究表明，鼻周皮层与新皮层和内皮层形成了很强的相互联系。相反，生理学研究结果表明，新皮质和内嗅输入的鼻周传播发生的概率极低。该提案的总体目标是：（A）阐明限制通过鼻皮质的脉冲流量的抑制机制，以及（B）识别允许鼻皮质克服这种抑制的传入神经，重点关注来自内侧的输入前额皮质 (mPFC) 和杏仁核。为此，我们将：（1）使用颞新皮质或特定鼻区中的顺行（或逆行）示踪剂注射与电子处的免疫细胞化学结合来识别新皮质和内嗅轴突到区域35、36和EC的递质和突触目标。微观层面。 (2)确定新皮质和内嗅刺激对鼻神经元的影响，体外用全细胞贴片法记录，体内用锋利的微量移液器记录。 (3-4) 确定 mPFC 和杏仁核如何影响穿过鼻皮质的脉冲流量。这将通过解剖学和生理学实验的结合来研究。解剖实验将需要顺行示踪剂注射仅限于 mPFC 和杏仁核的特定区域，并且方法与 (1) 中相同。生理实验将涉及 mPFC 或杏仁核中印防己毒素的反向透析，以及体内鼻神经元的多个同时的细胞外和细胞内神经元记录。由于鼻皮质主要在阿尔茨海默病的早期阶段受损，因此这项基础研究计划可能会提高我们对记忆障碍的理解。