Filamentous Fungi Genetic Analysis

丝状真菌遗传分析

• 批准号: 6958181
• 负责人: JENNIFER J. LOROS
• 金额: $ 21.62万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6958181
• 关键词: Neurospora; circadian rhythms; complementary DNA; developmental genetics; functional /structural genomics; fungal genetics; fungal proteins; genetic mapping; genetic polymorphism; genetic transcription; microorganism resource /registry; nucleic acid sequence; Neurospora; circadian rhythms; complementary DNA; developmental genetics; functional /structural genomics; fungal genetics; fungal proteins; genetic mapping; genetic polymorphism; genetic transcription; microorganism resource /registry; nucleic acid sequence

The aim of this Project is to develop and utilize oligonucleotide microarray technology for the model filamentous fungus Neurospora crassa. These studies will provide a solid foundation of gene expression profiles for the filamentous fungi that will promote comparative studies within this group and between the fungi and other organisms. The rich foundation of biological and molecular tools available for Neurospora has been complemented with an archival quality genomic sequence, thus creating the opportunity for asking questions of genome regulation on a global scale. Gene expression data generated by microarray technology as described in this proposal can measurably enhance the functional analysis of this organism's genes. Our first objective is to generate slides containing 70-met oligonucleotides representing each of the predicted genes of N. crassa and to use these slides to standardize our techniques to develop sound statistical analysis of microarray data. Our second objective is to carry out base-line transcriptional profiling on the sequenced strain of Neurospora following exposure to a variety of growth conditions and environmental stresses. Our third objective will test hypotheses derived from microarray experiments using knockout strains generated from Project #1 of this proposal. Anticipated knockouts will include (1) genes that have been annotated in N. crassa that are involved in transcriptional regulatory processes in S. cerevisiae and that have been transcriptionally profiled, (2) genes that are annotated as putative regulatory factors that are absent from S. cerevisiae, (3) homologs of genes that are absent from S. cerevisiae and S. pombe and are of unknown biological function in N. crassa, and (4) apparent filamentous fungal-specific genes that do not have homologs in other organisms. The work outlined in this project will provide procedures, computational tools, and baseline transcriptional profiling data for wild type Neurospora grown under a variety of conditions and will pave the way for collaborative studies in the future. In addition to fostering similar studies in other filamentous fungi, these data will expedite the functional analysis of fungi in general including important human pathogens.

该项目的目的是开发和利用寡核苷酸微阵列技术，用于模型丝状真菌Neurospora Crassa。这些研究将为丝状真菌提供稳固的基因表达谱，这将促进该组内以及真菌与其他生物之间的比较研究。可用于Neurospora的生物学和分子工具的丰富基础与档案质量基因组序列相辅相成，从而为在全球范围内提出基因组调节问题创造了机会。 如本提案所述，由微阵列技术产生的基因表达数据可以衡量地增强对该生物体基因的功能分析。我们的第一个目的是生成含有代表N. crassa的每个预测基因的70米寡核苷酸的幻灯片，并使用这些幻灯片来标准化我们的技术以开发微阵列数据的合理统计分析。我们的第二个目标是在暴露于各种生长条件和环境应力后，对神经孢子的测序菌株进行基础线转录分析。我们的第三个目标将使用该提案项目1产生的敲除菌株从微阵列实验中得出的假设进行测试。预期的淘汰赛将包括（1）已在N. crassa中注释的基因 参与酿酒酵母中的转录调节过程，并已转录特征，（2）被注释为cervisiae的假定调节因素，（（3）cervisiae and S. pombe和S. Pombe和Spific Bific Bific fenestific n. Crassa and crassa和4.4. crassa and（4）基因的同源物（4）在其他生物体中没有同源物。 该项目中概述的工作将为在各种条件下生长的野生型Neurospora提供程序，计算工具和基线转录分析数据，并将为未来的协作研究铺平道路。除了培养其他丝状真菌中的类似研究外，这些数据还将加快真菌的功能分析，包括重要的人类病原体。