Phenotyping of Mice Using Bioimaging

使用生物成像对小鼠进行表型分析

• 批准号: 6966250
• 负责人: Guo-Qiang ZHANG
• 金额: $ 16.41万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6966250
• 关键词: adipose tissue; bioimaging /biomedical imaging; bone development; bone imaging /visualization /scanning; computed axial tomography; computer data analysis; genetic models; image processing; laboratory mouse; morphometry; phenotype; statistics /biometry; technology /technique development

The goal is for the candidate to embark on a career transformation by acquiring critical knowledge and skills in biomedical sciences and become a productive and successful investigator in emerging biomedical areas with a unique set of expertises. One of the important components consists of projects in genetic phenotyping of small animals using in vivo bioimaging. The research will first tackle the technically easier problem of measuring and analyzing regional fat distribution in mice so as to identify potential subgroups with specific health problems; e.g., increased levels of visceral fat have been associated with insulin resistance, cardiovascular disease, and other metabolic syndromes. Building on this experience, the project will proceed to the identification of skeletal phenotypes using 3D micro-CT imaging. Not only will image segmentation be a challenge, a paradigm for extracting complex anatomical information will be created, as for example from a mouse skeleton, and clustering data into phenotypes for comparison with genotypes. This paradigm will set a standard and open new possibilities in deriving biomedical knowledge from mouse models.

目标是让候选人通过获得生物医学科学的关键知识和技能来开始职业转型，并成为新兴生物医学领域富有成效和成功的研究人员，并拥有一套独特的专业知识。其中重要组成部分之一是利用体内生物成像对小动物进行遗传表型分析的项目。该研究将首先解决技术上更简单的问题，即测量和分析小鼠的区域脂肪分布，以确定具有特定健康问题的潜在亚组；例如，内脏脂肪水平增加与胰岛素抵抗、心血管疾病和其他代谢综合征有关。 在此经验的基础上，该项目将继续使用 3D 显微 CT 成像来识别骨骼表型。图像分割不仅是一个挑战，还将创建一个用于提取复杂解剖信息的范例，例如从小鼠骨骼中提取复杂的解剖信息，并将数据聚类为表型以与基因型进行比较。这一范式将为从小鼠模型中获取生物医学知识设定标准并开辟新的可能性。