Skin protein turnover in older adults

老年人皮肤蛋白质周转率

• 批准号: 6828496
• 负责人: Elena Volpi
• 金额: $ 7.55万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6828496
• 关键词: age difference; aging; biopsy; chemical kinetics; clinical research; collagen; gas chromatography mass spectrometry; human old age (65+); human subject; ion exchange chromatography; male; phenylalanine; proline; protein biosynthesis; protein metabolism; protein purification; skin; stable isotope; wound healing; young adult human (21-34)

DESCRIPTION (provided by applicant): The goal of this proposal is to gather preliminary data to test the feasibility and calculate the sample size for a larger study on the effects of age on skin protein turnover and wound healing in humans. Our general hypothesis is that dermal collagen turnover changes with aging and these changes are responsible for many of the alterations in skin function found in older adults, which may increase the risk of mechanical injury and delayed wound healing. Therefore, the measurement of dermal collagen turnover may be used as an objective tool to determine the effects of age on the skin, quantify the rate of wound healing, and test the effects of specific interventions aimed at stimulating skin regeneration and repair. Since collagen makes up the bulk of skin proteins, we further hypothesize that mixed skin protein kinetics can reliably reflect the turnover rate of skin collagen, and thus be used as a surrogate measure of skin collagen turnover. The specific aims of this proposal will be (1) to determine whether mixed skin protein turnover can be used as a surrogate measure of skin collagen turnover in human subjects, and (2) to determine whether skin protein synthesis is slower and/or breakdown faster in older subjects as compared to younger controls. To accomplish our aims we will simultaneously measure the synthesis and breakdown rates of mixed skin proteins, and the skin collagen synthesis rate in healthy human subjects using a new stable isotope method. The data obtained with this pilot project will be used as a basis for further studies (to pursue using the R01 mechanism) on the effects of aging on skin metabolism and wound healing.

描述（由申请人提供）：该提案的目的是收集初步数据来测试可行性并计算样本量，以进行更大规模的研究，研究年龄对人类皮肤蛋白质周转和伤口愈合的影响。 我们的一般假设是真皮胶原蛋白周转率随着衰老而变化，这些变化是 老年人皮肤功能的许多改变是由它引起的，这可能会增加机械损伤和伤口愈合延迟的风险。因此，真皮胶原更新的测量可用作确定年龄对皮肤的影响、量化伤口愈合率以及测试旨在刺激皮肤再生和修复的特定干预措施的效果的客观工具。由于胶原蛋白构成了皮肤蛋白的大部分，我们进一步假设混合皮肤蛋白动力学可以可靠地反映皮肤胶原蛋白的周转率，从而可用作皮肤胶原蛋白周转率的替代指标。 该提案的具体目标是（1）确定混合皮肤蛋白周转率是否可以用作人类受试者皮肤胶原蛋白周转率的替代指标，以及（2）确定皮肤蛋白合成是否较慢和/或分解较快与年轻对照组相比，老年受试者。 为了实现我们的目标，我们将使用新的稳定同位素方法同时测量健康人类受试者中混合皮肤蛋白质的合成和分解率以及皮肤胶原蛋白合成率。 通过该试点项目获得的数据将用作进一步研究的基础（以继续使用 R01机制）关于衰老对皮肤代谢和伤口愈合的影响。