Potassium Channels and Dendritic Function in Hippocampa*

海马中的钾通道和树突功能*

• 批准号: 6813986
• 负责人: Dax A Hoffman
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6813986
• 关键词: dendrites; electrophysiology; hippocampus; neural plasticity; neural transmission; potassium channel; synapses; voltage /patch clamp; voltage gated channel

A nonuniform density of subthreshold, rapidly inactivating potassium channels regulates signal propagation in the dendrites of CA1 pyramidal neurons of the hippocampus. This nonuniform distribution (with higher expression in the dendrites than in the soma) means that the electrical properties of the dendrites are markedly different from those of the soma. Incoming synaptic signals are shaped by the activity of these channels, and action potentials, once initiated in the axon, progressively decrease in amplitude as they propagate back into the dendrites. By combining patch clamp recording in brain slices of the hippocampus with molecular biology techniques, the Molecular Neurophysiology and Biophysics Unit investigates the electrophysiological properties and molecular nature of the voltage-gated channels expressed in CA1 dendrites, how their expression is regulated, and their role in synaptic integration and plasticity.

阈下密度不均匀、快速失活的钾通道调节海马 CA1 锥体神经元树突中的信号传播。这种不均匀的分布（树突中的表达高于胞体中的表达）意味着树突的电特性与胞体的电特性明显不同。输入的突触信号由这些通道的活动决定，而动作电位一旦在轴突中启动，随着它们传播回树突，其幅度逐渐减小。通过将海马脑切片中的膜片钳记录与分子生物学技术相结合，分子神经生理学和生物物理学单元研究了 CA1 树突中表达的电压门控通道的电生理特性和分子性质、它们的表达如何被调节以及它们在突触整合和可塑性。